ID   NORB_STAAE              Reviewed;         463 AA.
AC   A6QGY6;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Quinolone resistance protein NorB;
GN   Name=norB; OrderedLocusNames=NWMN_1346;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
RN   [2]
RP   INDUCTION BY MGRA.
RX   PubMed=16484201; DOI=10.1128/jb.188.5.1899-1910.2006;
RA   Luong T.T., Dunman P.M., Murphy E., Projan S.J., Lee C.Y.;
RT   "Transcription profiling of the mgrA regulon in Staphylococcus aureus.";
RL   J. Bacteriol. 188:1899-1910(2006).
CC   -!- FUNCTION: Multidrug efflux pump that acts independently of NorA and is
CC       one of the factors that confers resistance against diverse quinolones
CC       and chemical compounds. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Up-regulated by MgrA. {ECO:0000269|PubMed:16484201}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; AP009351; BAF67618.1; -; Genomic_DNA.
DR   RefSeq; WP_000414685.1; NZ_CP023390.1.
DR   AlphaFoldDB; A6QGY6; -.
DR   SMR; A6QGY6; -.
DR   EnsemblBacteria; BAF67618; BAF67618; NWMN_1346.
DR   KEGG; sae:NWMN_1346; -.
DR   HOGENOM; CLU_000960_28_3_9; -.
DR   OMA; RYPRFVG; -.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..463
FT                   /note="Quinolone resistance protein NorB"
FT                   /id="PRO_0000361965"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        435..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   463 AA;  49293 MW;  D48070208701CD5C CRC64;
     MEKPSREAFE GNNKLLIGIV LSVITFWLFA QSLVNVVPIL EDSFNTDIGT VNIAVSITAL
     FSGMFVVGAG GLADKYGRIK LTNIGIILNI LGSLLIIISN IPLLLIIGRL IQGLSAACIM
     PATLSIIKSY YIGKDRQRAL SYWSIGSWGG SGVCSFFGGA VATLLGWRWI FILSIIISLI
     ALFLIKGTPE TKSKSISLNK FDIKGLVLLV IMLLSLNILI TKGSELGVTS LLFITLLAIA
     IGSFSLFIVL EKRATNPLID FKLFKNKAYT GATASNFLLN GVAGTLIVAN TFVQRGLGYS
     SLQAGSLSIT YLVMVLIMIR VGEKLLQTLG CKKPMLIGTG VLIVGECLIS LTFLPEIFYV
     ICCIIGYLFF GLGLGIYATP STDTAIANAP LEKVGVAAGI YKMASALGGA FGVALSGAVY
     AIVSNMTNIY TGAMIALWLN AGMGILSFVI ILLLVPKQND TQL