ID   NORB_STAAW              Reviewed;         463 AA.
AC   Q8NWQ5;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Quinolone resistance protein NorB;
GN   Name=norB; OrderedLocusNames=MW1325;
OS   Staphylococcus aureus (strain MW2).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=196620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MW2;
RX   PubMed=12044378; DOI=10.1016/s0140-6736(02)08713-5;
RA   Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y.,
RA   Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K.;
RT   "Genome and virulence determinants of high virulence community-acquired
RT   MRSA.";
RL   Lancet 359:1819-1827(2002).
RN   [2]
RP   FUNCTION IN BACTERIAL SURVIVAL WITHIN AN ABSCESS, AND INDUCTION.
RX   PubMed=18723624; DOI=10.1128/jb.00655-08;
RA   Ding Y., Onodera Y., Lee J.C., Hooper D.C.;
RT   "NorB, an efflux pump in Staphylococcus aureus strain MW2, contributes to
RT   bacterial fitness in abscesses.";
RL   J. Bacteriol. 190:7123-7129(2008).
CC   -!- FUNCTION: Multidrug efflux pump that acts independently of NorA and is
CC       one of the factors that confers resistance against diverse quinolones
CC       and chemical compounds (By similarity). Can facilitate bacterial
CC       survival in vivo when overexpressed in an abscess and may contribute to
CC       the relative resistance of staphylococcal abscesses to antimicrobial
CC       therapy. {ECO:0000250, ECO:0000269|PubMed:18723624}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Transcription is highly induced in abscesses. Up-regulated
CC       by MgrA. Down-regulated during stationary-phase.
CC       {ECO:0000269|PubMed:18723624}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; BA000033; BAB95190.1; -; Genomic_DNA.
DR   RefSeq; WP_000414682.1; NC_003923.1.
DR   AlphaFoldDB; Q8NWQ5; -.
DR   SMR; Q8NWQ5; -.
DR   EnsemblBacteria; BAB95190; BAB95190; BAB95190.
DR   KEGG; sam:MW1325; -.
DR   HOGENOM; CLU_000960_28_3_9; -.
DR   OMA; RYPRFVG; -.
DR   Proteomes; UP000000418; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport; Virulence.
FT   CHAIN           1..463
FT                   /note="Quinolone resistance protein NorB"
FT                   /id="PRO_0000361962"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        435..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   463 AA;  49245 MW;  2FE8511AC4A5F4F2 CRC64;
     MEKPSREAFE GNNKLLIGIV LSVITFWLFA QSLVNVVPIL EDSFNTDIGT VNIAVSITAL
     FSGMFVVGAG GLADKYGRIK LTNIGIILNI LGSLLIIISN IPLLLIIGRL IQGLSAACIM
     PATLSIIKSY YIGKDRQRAL SYWSIGSWGG SGVCSFFGGA VATLLGWRWI FILSIIISLI
     ALFLIKGTPE TKSKSISLNK FDIKGLVLLV IMLLSLNILI TKGSELGVSS LLFITLLAIA
     IGSFSLFIVL EKRATNPLID FKLFKNKAYT GATASNFLLN GVAGTLIVAN TFVQRGLGYS
     SLQAGSLSIT YLVMVLIMIR VGEKLLQTLG CKKPMLIGTG VLIVGECLIS LTFLPEILYV
     ICCIIGYLFF GLGLGIYATP STDTAIANAP LEKVGVAAGI YKMASALGGA FGVALSGAVY
     AIVSNMTNIY TGAMIALWLN AGMGILSFVI ILLLVPKQND TQL