NORM_VIBCH
ID NORM_VIBCH Reviewed; 457 AA.
AC Q9KRU4; Q8VUU4;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Multidrug resistance protein NorM;
DE AltName: Full=Multidrug-efflux transporter;
DE AltName: Full=Na(+)/drug antiporter;
GN Name=norM; Synonyms=vcmA; OrderedLocusNames=VC_1540;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=NCTC 4716;
RX PubMed=11583854; DOI=10.1111/j.1574-6968.2001.tb10847.x;
RA Huda M.N., Morita Y., Kuroda T., Mizushima T., Tsuchiya T.;
RT "Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-
RT halophilic bacterium.";
RL FEMS Microbiol. Lett. 203:235-239(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Multidrug efflux pump that functions as a Na(+)/drug
CC antiporter. Confers resistance to norfloxacin, ciprofloxacin,
CC ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium
CC bromide and acriflavine. {ECO:0000269|PubMed:11583854}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC 2.A.66.1) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF94694.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB063193; BAB79260.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF94694.1; ALT_INIT; Genomic_DNA.
DR PIR; H82186; H82186.
DR RefSeq; NP_231180.2; NC_002505.1.
DR RefSeq; WP_000555132.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KRU4; -.
DR SMR; Q9KRU4; -.
DR STRING; 243277.VC_1540; -.
DR TCDB; 2.A.66.1.2; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR DNASU; 2613919; -.
DR EnsemblBacteria; AAF94694; AAF94694; VC_1540.
DR KEGG; vch:VC_1540; -.
DR PATRIC; fig|243277.26.peg.1469; -.
DR eggNOG; COG0534; Bacteria.
DR HOGENOM; CLU_012893_6_0_6; -.
DR OMA; EFWILLK; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR002528; MATE_fam.
DR Pfam; PF01554; MatE; 2.
DR PIRSF; PIRSF006603; DinF; 1.
DR TIGRFAMs; TIGR00797; matE; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Antiport; Cell inner membrane; Cell membrane;
KW Ion transport; Membrane; Reference proteome; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..457
FT /note="Multidrug resistance protein NorM"
FT /id="PRO_0000164244"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 100
FT /note="V -> I (in strain: NCTC 4716)"
SQ SEQUENCE 457 AA; 49424 MW; A48708AFA49678D6 CRC64;
MHRYKKEASN LIKLATPVLI ASVAQTGMGF VDTIMAGGVS AIDMAAVSIA ASIWLPSILF
GVGLLMALVP VVAQLNGAGR QHKIPFEVHQ GLILALLVSV PIIAVLFQTQ FIIRFMDVEE
AMATKTVGYM HAVIFAVPAY LLFQALRSFT DGMSLTKPAM VIGFIGLLLN IPLNWIFVYG
KFGAPELGGV GCGVATAIVY WIMLLLLLFY IVTSKRLAHV KVFETFHKPQ PKELIRLFRL
GFPVAAALFF EVTLFAVVAL LVAPLGSTVV AAHQVALNFS SLVFMFPMSI GAAVSIRVGH
KLGEQDTKGA AIAANVGLMT GLATACITAL LTVLFREQIA LLYTENQVVV ALAMQLLLFA
AIYQCMDAVQ VVAAGSLRGY KDMTAIFHRT FISYWVLGLP TGYILGMTNW LTEQPLGAKG
FWLGFIIGLS AAALMLGQRL YWLQKQSDDV QLHLAAK
