NORM_VIBPA
ID NORM_VIBPA Reviewed; 456 AA.
AC O82855;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2003, sequence version 2.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Multidrug resistance protein NorM;
DE AltName: Full=Multidrug-efflux transporter;
DE AltName: Full=Na(+)/drug antiporter;
GN Name=norM; OrderedLocusNames=VP1479;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=AQ3334;
RX PubMed=9661020; DOI=10.1128/aac.42.7.1778;
RA Morita Y., Kodama K., Shiota S., Mine T., Kataoka A., Mizushima T.,
RA Tsuchiya T.;
RT "NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and
RT its homolog in Escherichia coli.";
RL Antimicrob. Agents Chemother. 42:1778-1782(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
RN [3]
RP IDENTIFICATION OF THE MATE FAMILY.
RX PubMed=9987140; DOI=10.1046/j.1365-2958.1999.01162.x;
RA Brown M.H., Paulsen I.T., Skurray R.A.;
RT "The multidrug efflux protein NorM is a prototype of a new family of
RT transporters.";
RL Mol. Microbiol. 31:394-395(1999).
RN [4]
RP CHARACTERIZATION.
RC STRAIN=AQ3334;
RX PubMed=11073914; DOI=10.1128/jb.182.23.6694-6697.2000;
RA Morita Y., Kataoka A., Shiota S., Mizushima T., Tsuchiya T.;
RT "NorM of Vibrio parahaemolyticus is an Na(+)-driven multidrug efflux
RT pump.";
RL J. Bacteriol. 182:6694-6697(2000).
CC -!- FUNCTION: Multidrug efflux pump that functions as a Na(+)/drug
CC antiporter. Confers resistance to several drugs, such as norfloxacin,
CC ciprofloxacin, ethidium, kanamycin and streptomycin.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC 2.A.66.1) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB010463; BAA31456.1; -; Genomic_DNA.
DR EMBL; BA000031; BAC59742.1; -; Genomic_DNA.
DR RefSeq; NP_797858.1; NC_004603.1.
DR RefSeq; WP_005483441.1; NC_004603.1.
DR AlphaFoldDB; O82855; -.
DR SMR; O82855; -.
DR STRING; 223926.28806470; -.
DR TCDB; 2.A.66.1.1; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR EnsemblBacteria; BAC59742; BAC59742; BAC59742.
DR GeneID; 1188986; -.
DR KEGG; vpa:VP1479; -.
DR PATRIC; fig|223926.6.peg.1412; -.
DR eggNOG; COG0534; Bacteria.
DR HOGENOM; CLU_012893_6_0_6; -.
DR OMA; EFWILLK; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR002528; MATE_fam.
DR Pfam; PF01554; MatE; 2.
DR PIRSF; PIRSF006603; DinF; 1.
DR TIGRFAMs; TIGR00797; matE; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Antiport; Cell inner membrane; Cell membrane;
KW Ion transport; Membrane; Reference proteome; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..456
FT /note="Multidrug resistance protein NorM"
FT /id="PRO_0000164246"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 40..41
FT /note="SA -> TQ (in Ref. 1; BAA31456)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="G -> D (in Ref. 1; BAA31456)"
FT /evidence="ECO:0000305"
FT CONFLICT 351
FT /note="S -> T (in Ref. 1; BAA31456)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 456 AA; 49280 MW; AC57495250BDBA59 CRC64;
MHRYKEEASS LIKLATPVLI ASVAQTGMGF VDTVMAGGVS ATDMAAVSVA SSIWLPSILF
GIGLLMALVP VVAQLNGSAR REKIPFEIQQ GVVLALLISI PIIGVLLQTQ FILQLMDVEA
VMAGKTVGYI HAVIFAVPAF LLFQTLRSFT DGMSLTKPAM VIGFIGLLLN IPLNWIFVYG
KFGAPELGGV GCGVATTIVY WVMFALLLAY VMTSSRLKSI NVFGEYHKPQ WKAQVRLFKL
GFPVAAALFF EVTLFAVVAL LVSPLGPIIV AAHQVAINFS SLVFMLPMSV GAAVSIRVGH
RLGEENVDGA RVASRVGIMV GLALATITAI ITVLSRELIA ELYTNNPEVI SLAMQLLLFA
AVYQCTDAVQ VIAAGALRGY KDMRAIFNRT FIAYWILGLP TGYILGRTDW IVEPMGAQGF
WLGFIIGLTA AALMLGVRLR WMHRQEPDVQ LNFSLQ
