ID   NORW_ALIF1              Reviewed;         381 AA.
AC   Q5E3X0;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Nitric oxide reductase FlRd-NAD(+) reductase {ECO:0000255|HAMAP-Rule:MF_01313};
DE            EC=1.18.1.- {ECO:0000255|HAMAP-Rule:MF_01313};
DE   AltName: Full=Flavorubredoxin reductase {ECO:0000255|HAMAP-Rule:MF_01313};
DE            Short=FlRd-reductase {ECO:0000255|HAMAP-Rule:MF_01313};
DE            Short=FlavoRb reductase {ECO:0000255|HAMAP-Rule:MF_01313};
GN   Name=norW {ECO:0000255|HAMAP-Rule:MF_01313};
GN   Synonyms=flrR {ECO:0000255|HAMAP-Rule:MF_01313}; OrderedLocusNames=VF_1781;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: One of at least two accessory proteins for anaerobic nitric
CC       oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase.
CC       {ECO:0000255|HAMAP-Rule:MF_01313}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + NAD(+) + 2 reduced [nitric oxide reductase rubredoxin
CC         domain] = NADH + 2 oxidized [nitric oxide reductase rubredoxin
CC         domain]; Xref=Rhea:RHEA:42960, Rhea:RHEA-COMP:10304, Rhea:RHEA-
CC         COMP:10305, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01313};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01313};
CC   -!- PATHWAY: Nitrogen metabolism; nitric oxide reduction.
CC       {ECO:0000255|HAMAP-Rule:MF_01313}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01313}.
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01313}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW86276.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000020; AAW86276.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_047863521.1; NC_006840.2.
DR   RefSeq; YP_205164.1; NC_006840.2.
DR   AlphaFoldDB; Q5E3X0; -.
DR   SMR; Q5E3X0; -.
DR   STRING; 312309.VF_1781; -.
DR   PRIDE; Q5E3X0; -.
DR   EnsemblBacteria; AAW86276; AAW86276; VF_1781.
DR   KEGG; vfi:VF_1781; -.
DR   PATRIC; fig|312309.11.peg.1807; -.
DR   eggNOG; COG0446; Bacteria.
DR   HOGENOM; CLU_003291_4_4_6; -.
DR   OMA; IHHFWTF; -.
DR   OrthoDB; 1149616at2; -.
DR   UniPathway; UPA00638; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016731; F:oxidoreductase activity, acting on iron-sulfur proteins as donors, NAD or NADP as acceptor; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_01313; NorW; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR023961; NO_rdtase_NorW.
DR   InterPro; IPR041364; Rbx-bd.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF18113; Rbx_binding; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..381
FT                   /note="Nitric oxide reductase FlRd-NAD(+) reductase"
FT                   /id="PRO_0000305614"
SQ   SEQUENCE   381 AA;  41392 MW;  3F1CAD24B3FDF579 CRC64;
     MSLPIVIIGS GFASYQLIKT IRRTNNDCPI HVFTNDSGDD YNKPDLSHVF SKQQSPEEVV
     TLSGGDFAEQ YNVILHRHTW VESIDTEIQA ITANGEQYAY GKLVLATGSQ TFIPPFHGDG
     CGDILTLNSL KEFAGIQQKV LESKKVLVVG GGLIGTELAM DLANAGKMVT LVEPNTHLLA
     NMVPDFISLP LEKACKEKGI TVNLSDCVQA VNKQEQGYRV TTSNGHSYYV DCVISAAGLK
     PNTKLATEAN LMVNRGIVVD LNLQTSANNI YALGDCAEIE GKVMAYLQPI LLSANALAKT
     LLGTDTALSM PNMMVKVKTP NYPIQLAGNT STDIERWSVD IDTQGLCAKA YNINNQLTGF
     VVTNERVKNA FPLFRELNTT N