ID   NOSIP_BOVIN             Reviewed;         302 AA.
AC   Q3SWY5;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Nitric oxide synthase-interacting protein;
DE   AltName: Full=E3 ubiquitin-protein ligase NOSIP;
DE            EC=2.3.2.27;
DE   AltName: Full=RING-type E3 ubiquitin transferase NOSIP {ECO:0000305};
GN   Name=NOSIP;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase that is essential for proper
CC       development of the forebrain, the eye, and the face. Catalyzes
CC       monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A)
CC       catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates
CC       nitric oxide production by inducing NOS1 and NOS3 translocation to
CC       actin cytoskeleton and inhibiting their enzymatic activity (By
CC       similarity). {ECO:0000250|UniProtKB:Q9D6T0,
CC       ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- SUBUNIT: Interacts with NOS1 and NOS3 (By similarity). Interacts with
CC       PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A
CC       subunits (By similarity). {ECO:0000250|UniProtKB:Q9D6T0,
CC       ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y314}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9Y314}. Note=Translocates from nucleus to
CC       cytoplasm in the G2 phase of the cell cycle.
CC       {ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- DOMAIN: The U-box-like region is a truncated U-box domain. It is
CC       unknown whether it is functional or not.
CC   -!- SIMILARITY: Belongs to the NOSIP family. {ECO:0000305}.
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DR   EMBL; BC104600; AAI04601.1; -; mRNA.
DR   RefSeq; NP_001068970.1; NM_001075502.1.
DR   AlphaFoldDB; Q3SWY5; -.
DR   STRING; 9913.ENSBTAP00000019215; -.
DR   PaxDb; Q3SWY5; -.
DR   Ensembl; ENSBTAT00000019215; ENSBTAP00000019215; ENSBTAG00000014450.
DR   GeneID; 511234; -.
DR   KEGG; bta:511234; -.
DR   CTD; 51070; -.
DR   VEuPathDB; HostDB:ENSBTAG00000014450; -.
DR   VGNC; VGNC:32175; NOSIP.
DR   eggNOG; KOG3039; Eukaryota.
DR   GeneTree; ENSGT00390000015505; -.
DR   HOGENOM; CLU_053742_0_0_1; -.
DR   InParanoid; Q3SWY5; -.
DR   OMA; AKEKRPM; -.
DR   OrthoDB; 1567031at2759; -.
DR   TreeFam; TF314268; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000014450; Expressed in laryngeal cartilage and 107 other tissues.
DR   ExpressionAtlas; Q3SWY5; baseline.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 2.
DR   InterPro; IPR016818; NOSIP.
DR   InterPro; IPR031790; Znf-NOSIP.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR13063; PTHR13063; 1.
DR   Pfam; PF15906; zf-NOSIP; 1.
DR   PIRSF; PIRSF023577; ENOS_interacting; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Nucleus; Phosphoprotein;
KW   Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..302
FT                   /note="Nitric oxide synthase-interacting protein"
FT                   /id="PRO_0000280584"
FT   REGION          55..75
FT                   /note="U-box-like"
FT   REGION          126..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           78..101
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         36
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y314"
SQ   SEQUENCE   302 AA;  33043 MW;  D9016EA44C606FD8 CRC64;
     MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP
     DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGARREEQK ELQRAAAQDH VRGFLEKEAA
     IVSRPLNPFT PKAASAGNGP DDAQPGSSAG PAGKDKDKAL PSFWIPSLTP EAKATKLEKP
     SRIVTCPMSG KPLRMSDLTP VRFTPLDSSV DRVGLITRSE RYVCAVTRDS LSNATPCAVL
     RPSGAVVTLE CVEKLIRKDM VDPVTGEKLT DRDIIVLQRG GTGFAGSGVK LQAEKSRPVM
     QA