ID   NOSIP_DANRE             Reviewed;         304 AA.
AC   Q5U3S7;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Nitric oxide synthase-interacting protein;
DE   AltName: Full=E3 ubiquitin-protein ligase NOSIP;
DE            EC=2.3.2.27;
DE   AltName: Full=RING-type E3 ubiquitin transferase NOSIP {ECO:0000305};
GN   Name=nosip; ORFNames=zgc:101669;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase that is essential for proper
CC       development of the forebrain, the eye, and the face (By similarity).
CC       Negatively regulates nitric oxide production by inducing nitric oxide
CC       synthase translocation to actin cytoskeleton and inhibiting its
CC       enzymatic activity (By similarity). {ECO:0000250|UniProtKB:Q9D6T0,
CC       ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y314}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9Y314}. Note=Translocates from nucleus to
CC       cytoplasm in the G2 phase of the cell cycle.
CC       {ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- SIMILARITY: Belongs to the NOSIP family. {ECO:0000305}.
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DR   EMBL; BC085409; AAH85409.1; -; mRNA.
DR   RefSeq; NP_001007435.1; NM_001007434.1.
DR   RefSeq; XP_005164079.1; XM_005164022.3.
DR   RefSeq; XP_009297811.1; XM_009299536.2.
DR   AlphaFoldDB; Q5U3S7; -.
DR   STRING; 7955.ENSDARP00000055311; -.
DR   PaxDb; Q5U3S7; -.
DR   DNASU; 492793; -.
DR   Ensembl; ENSDART00000055312; ENSDARP00000055311; ENSDARG00000037958.
DR   Ensembl; ENSDART00000165909; ENSDARP00000139361; ENSDARG00000037958.
DR   Ensembl; ENSDART00000186409; ENSDARP00000157364; ENSDARG00000112335.
DR   GeneID; 492793; -.
DR   KEGG; dre:492793; -.
DR   CTD; 51070; -.
DR   ZFIN; ZDB-GENE-041114-146; nosip.
DR   eggNOG; KOG3039; Eukaryota.
DR   GeneTree; ENSGT00390000015505; -.
DR   InParanoid; Q5U3S7; -.
DR   OMA; AKEKRPM; -.
DR   OrthoDB; 1567031at2759; -.
DR   PhylomeDB; Q5U3S7; -.
DR   TreeFam; TF314268; -.
DR   PRO; PR:Q5U3S7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 3.
DR   Bgee; ENSDARG00000037958; Expressed in mature ovarian follicle and 22 other tissues.
DR   ExpressionAtlas; Q5U3S7; baseline.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 2.
DR   InterPro; IPR016818; NOSIP.
DR   InterPro; IPR031790; Znf-NOSIP.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR13063; PTHR13063; 1.
DR   Pfam; PF15906; zf-NOSIP; 1.
DR   PIRSF; PIRSF023577; ENOS_interacting; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Nucleus; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..304
FT                   /note="Nitric oxide synthase-interacting protein"
FT                   /id="PRO_0000280588"
FT   REGION          94..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           78..101
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        94..119
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        120..165
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   304 AA;  33332 MW;  7A84704922F9FCB6 CRC64;
     MTRHGKNCTA GAVYTYHEKR KDTAASGYGT QSVRLGKDAI KDFDCCSLSL QPCRDPVLTE
     DGYLYEKEAI LQYILHQKTE IAKKMKAYEK QKQALKSEGQ LESKSEERER AEKFKQRENN
     IVSKPINPFT SGKSKDEGNQ NGSTSSSSTD TSSGESSSSS ALPSFWIPSL TPEAKPTLLK
     KPSKTVSCPM SGRPLKMSDL ITVRFTPLDP SLDRVALLTR QDRYVCAVTK DTLGNSVPCA
     VLRPSGVVVT MECVEKLIKK DMVDPITGDK LKEKDIIPIQ RGGTGFAGSG VDLKAKEARP
     VMQA