ID   NOSIP_DICDI             Reviewed;         333 AA.
AC   Q55DU4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Nitric oxide synthase-interacting protein homolog;
GN   Name=nosip; ORFNames=DDB_G0270882;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA   Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA   Ivens A., Martinez J.L., Escalante R.;
RT   "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT   and specific effects from PAO1 and PA14 strains.";
RL   BMC Microbiol. 8:109-109(2008).
RN   [3]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA   Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA   Skelton J., Ivens A., Bozzaro S.;
RT   "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT   bacteria in Dictyostelium.";
RL   BMC Genomics 9:291-291(2008).
CC   -!- FUNCTION: Regulates nitric oxide production. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- INDUCTION: Down-regulated by Pseudomonas aeruginosa, PAO1 strain and
CC       PA14 strain infection and by growth on bacteria.
CC       {ECO:0000269|PubMed:18559084, ECO:0000269|PubMed:18590548}.
CC   -!- SIMILARITY: Belongs to the NOSIP family. {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72791.1; -; Genomic_DNA.
DR   RefSeq; XP_646037.1; XM_640945.1.
DR   AlphaFoldDB; Q55DU4; -.
DR   STRING; 44689.DDB0302416; -.
DR   PaxDb; Q55DU4; -.
DR   EnsemblProtists; EAL72791; EAL72791; DDB_G0270882.
DR   GeneID; 8616984; -.
DR   KEGG; ddi:DDB_G0270882; -.
DR   dictyBase; DDB_G0270882; nosip.
DR   eggNOG; KOG3039; Eukaryota.
DR   HOGENOM; CLU_053742_1_0_1; -.
DR   InParanoid; Q55DU4; -.
DR   OMA; AKEKRPM; -.
DR   PhylomeDB; Q55DU4; -.
DR   Reactome; R-DDI-203754; NOSIP mediated eNOS trafficking.
DR   PRO; PR:Q55DU4; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:dictyBase.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   GO; GO:0050999; P:regulation of nitric-oxide synthase activity; ISS:dictyBase.
DR   Gene3D; 3.30.40.10; -; 2.
DR   InterPro; IPR016818; NOSIP.
DR   InterPro; IPR031790; Znf-NOSIP.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR13063; PTHR13063; 1.
DR   Pfam; PF15906; zf-NOSIP; 1.
DR   PIRSF; PIRSF023577; ENOS_interacting; 1.
DR   SMART; SM00184; RING; 2.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..333
FT                   /note="Nitric oxide synthase-interacting protein homolog"
FT                   /id="PRO_0000380228"
FT   REGION          120..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          73..119
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        120..174
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   333 AA;  37764 MW;  C1DB8F1A0893F567 CRC64;
     MPRHSKHSCS NSGKFSNYER QLLKYGTTTE RLGKDSIKNF DSCSLCLNDV IMPVICNKGH
     LYCKECILTS LIDQKKLIKI KEKEWEQYQN KLKYEQDQKQ EKQHQDSIKE FEKNVITLEG
     IGSNDDNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTTTTTTTTT TTSDVNNKDK
     EKKEIVEKEI KLNSYWVITP DNKDKAIEKP RSYTVCPADG KHPLKSAQLI NVHFTNVKPS
     SSDSNDSNNQ YCCPICSKVL SNSTKTRLLK RCGHVFCSCL DKFKEDSSSQ LSCYVCDKPY
     TEDEIIQIHS GGTGFSGSGS NLEAKKYTHT AIV