ID   NOSIP_XENLA             Reviewed;         298 AA.
AC   Q6NUH3;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Nitric oxide synthase-interacting protein;
DE   AltName: Full=E3 ubiquitin-protein ligase NOSIP;
DE            EC=2.3.2.27;
DE   AltName: Full=RING-type E3 ubiquitin transferase NOSIP {ECO:0000305};
GN   Name=nosip;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase that is essential for proper
CC       development of the forebrain, the eye, and the face (By similarity).
CC       Negatively regulates nitric oxide production by inducing nitric oxide
CC       synthase translocation to actin cytoskeleton and inhibiting its
CC       enzymatic activity (By similarity). {ECO:0000250|UniProtKB:Q9D6T0,
CC       ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y314}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9Y314}. Note=Translocates from nucleus to
CC       cytoplasm in the G2 phase of the cell cycle.
CC       {ECO:0000250|UniProtKB:Q9Y314}.
CC   -!- SIMILARITY: Belongs to the NOSIP family. {ECO:0000305}.
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DR   EMBL; BC068614; AAH68614.1; -; mRNA.
DR   RefSeq; NP_001084604.1; NM_001091135.1.
DR   AlphaFoldDB; Q6NUH3; -.
DR   MaxQB; Q6NUH3; -.
DR   DNASU; 414557; -.
DR   GeneID; 414557; -.
DR   KEGG; xla:414557; -.
DR   CTD; 414557; -.
DR   Xenbase; XB-GENE-945894; nosip.S.
DR   Proteomes; UP000186698; Chromosome 7S.
DR   Bgee; 414557; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 2.
DR   InterPro; IPR016818; NOSIP.
DR   InterPro; IPR031790; Znf-NOSIP.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR13063; PTHR13063; 1.
DR   Pfam; PF15906; zf-NOSIP; 1.
DR   PIRSF; PIRSF023577; ENOS_interacting; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Nucleus; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..298
FT                   /note="Nitric oxide synthase-interacting protein"
FT                   /id="PRO_0000280589"
FT   REGION          132..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           78..101
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        139..156
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   298 AA;  33304 MW;  3B479DA95426A1D9 CRC64;
     MTRHGKNCTA GAVYTYYEKK KDTVASGYGT QTVRLSKDAV KDFDCCCLSL QPCKDPVVTS
     DGYIYEKESI LEYILHQKKE IARQMKAYEK QKNSKKAEME ELNKAAKESK MKAFLDKEMT
     IVSKPLNPFT RKSDSGIDAA EPSCSQQSSE EKNKQLPSFW IPSLTPEAKT SLVKKPDKTV
     YCPMSGRPLK MKDLMPVNFT RVDEKVDRVG LINRQDRYVC AVTRDMLGNS VPCAVLRPSG
     AVVTMECVEK LIKKDMIDPI SGDKLSERDI IMLQRGGTGF SGSGVLLQAK EARPVMQA