NOSIP_XENTR
ID NOSIP_XENTR Reviewed; 298 AA.
AC Q6P829;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Nitric oxide synthase-interacting protein;
DE AltName: Full=E3 ubiquitin-protein ligase NOSIP;
DE EC=2.3.2.27;
DE AltName: Full=RING-type E3 ubiquitin transferase NOSIP {ECO:0000305};
GN Name=nosip;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: E3 ubiquitin-protein ligase that is essential for proper
CC development of the forebrain, the eye, and the face (By similarity).
CC Negatively regulates nitric oxide production by inducing nitric oxide
CC synthase translocation to actin cytoskeleton and inhibiting its
CC enzymatic activity (By similarity). {ECO:0000250|UniProtKB:Q9D6T0,
CC ECO:0000250|UniProtKB:Q9Y314}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y314}. Nucleus
CC {ECO:0000250|UniProtKB:Q9Y314}. Note=Translocates from nucleus to
CC cytoplasm in the G2 phase of the cell cycle.
CC {ECO:0000250|UniProtKB:Q9Y314}.
CC -!- SIMILARITY: Belongs to the NOSIP family. {ECO:0000305}.
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DR EMBL; BC061402; AAH61402.1; -; mRNA.
DR RefSeq; NP_989029.1; NM_203698.1.
DR RefSeq; XP_012812096.1; XM_012956642.2.
DR AlphaFoldDB; Q6P829; -.
DR STRING; 8364.ENSXETP00000035744; -.
DR DNASU; 394625; -.
DR GeneID; 394625; -.
DR KEGG; xtr:394625; -.
DR CTD; 51070; -.
DR Xenbase; XB-GENE-945889; nosip.
DR InParanoid; Q6P829; -.
DR OrthoDB; 1567031at2759; -.
DR Reactome; R-XTR-203754; NOSIP mediated eNOS trafficking.
DR Proteomes; UP000008143; Chromosome 7.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000017642; Expressed in 4-cell stage embryo and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR Gene3D; 3.30.40.10; -; 2.
DR InterPro; IPR016818; NOSIP.
DR InterPro; IPR031790; Znf-NOSIP.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR13063; PTHR13063; 1.
DR Pfam; PF15906; zf-NOSIP; 1.
DR PIRSF; PIRSF023577; ENOS_interacting; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Nucleus; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..298
FT /note="Nitric oxide synthase-interacting protein"
FT /id="PRO_0000280590"
FT REGION 126..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 78..101
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 126..154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 298 AA; 33042 MW; 24AF64F7234EEC21 CRC64;
MTRHGKNCTA GAVYTYYEKK KDTAASGYGT QTVRLSKDAV KDFDCCCLSL QPCKDPVVTS
DGYIYEKEAI LEYILHQKKE IARQMKAYDK QKNAKKAEMD ELNKAAKESQ MKAFLDKEMT
IVSKPLNPFT RKSDSGADTA EPSGSQQSSE EKGKQLPSFW IPSLTPEAKT SLVKKPDKTV
YCPMSGKPLK MKDLIPVHFT AVDEKVDRVG LINRQDRYVC AVTRDMLGNS VPCAVLRPSG
AVVTMECVEK LIKKDMIDPI SGDKLHERDI IMLQRGGTGF SGSGVLLQAK EARPVMQA
