ID   NOSO_STAAC              Reviewed;         358 AA.
AC   Q5HEK7;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Nitric oxide synthase oxygenase;
DE            EC=1.14.14.47 {ECO:0000250|UniProtKB:O34453};
DE   AltName: Full=NOSoxy-like protein;
DE   AltName: Full=SANOS;
GN   Name=nos; OrderedLocusNames=SACOL1976;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Catalyzes the production of nitric oxide.
CC       {ECO:0000250|UniProtKB:O34453}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 L-arginine + 4 O2 + 3 reduced [flavodoxin] = 5 H(+) + 4 H2O
CC         + 2 L-citrulline + 2 nitric oxide + 3 oxidized [flavodoxin];
CC         Xref=Rhea:RHEA:52324, Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:10623,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16480, ChEBI:CHEBI:32682, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:57743, ChEBI:CHEBI:58210; EC=1.14.14.47;
CC         Evidence={ECO:0000250|UniProtKB:O34453};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:O34453};
CC   -!- COFACTOR:
CC       Name=(6S)-5,6,7,8-tetrahydrofolate; Xref=ChEBI:CHEBI:57453;
CC         Evidence={ECO:0000250|UniProtKB:O34453};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:O34453}.
CC   -!- MISCELLANEOUS: This protein is similar to the oxygenase domain of
CC       eukaryotic nitric oxide synthases but lacks the reductase domain which,
CC       in eukaryotes, is responsible for transfer of electrons to the ferric
CC       heme during nitric oxide synthesis.
CC   -!- SIMILARITY: Belongs to the NOS family. Bacterial NOS oxygenase
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP000046; AAW36946.1; -; Genomic_DNA.
DR   RefSeq; WP_000897635.1; NC_002951.2.
DR   AlphaFoldDB; Q5HEK7; -.
DR   SMR; Q5HEK7; -.
DR   EnsemblBacteria; AAW36946; AAW36946; SACOL1976.
DR   KEGG; sac:SACOL1976; -.
DR   HOGENOM; CLU_040293_0_0_9; -.
DR   OMA; IWNHQLI; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004517; F:nitric-oxide synthase activity; IEA:InterPro.
DR   GO; GO:0006809; P:nitric oxide biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.90.1230.10; -; 1.
DR   Gene3D; 3.90.340.10; -; 1.
DR   Gene3D; 3.90.440.10; -; 1.
DR   InterPro; IPR017142; Nitric_oxide_synthase_Oase-su.
DR   InterPro; IPR044943; NOS_dom_1.
DR   InterPro; IPR044940; NOS_dom_2.
DR   InterPro; IPR044944; NOS_dom_3.
DR   InterPro; IPR004030; NOS_N.
DR   InterPro; IPR036119; NOS_N_sf.
DR   Pfam; PF02898; NO_synthase; 1.
DR   PIRSF; PIRSF037219; NOS_oxygenase; 1.
DR   SUPFAM; SSF56512; SSF56512; 1.
DR   PROSITE; PS60001; NOS; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN           1..358
FT                   /note="Nitric oxide synthase oxygenase"
FT                   /id="PRO_0000170955"
FT   BINDING         62
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   358 AA;  41710 MW;  349A3A83E8D643A1 CRC64;
     MLFKEAQAFI ENMYKECHYE TQIINKRLHD IELEIKETGT YTHTEEELIY GAKMAWRNSN
     RCIGRLFWDS LNVIDARDVT DEASFLSSIT YHITQATNEG KLKPYITIYA PKDGPKIFNN
     QLIRYAGYDN CGDPAEKEVT RLANHLGWKG KGTNFDVLPL IYQLPNESVK FYEYPTSLIK
     EVPIEHNHYP KLRKLNLKWY AVPIISNMDL KIGGIVYPTA PFNGWYMVTE IGVRNFIDDY
     RYNLLEKVAD AFEFDTLKNN SFNKDRALVE LNYAVYHSFK KEGVSIVDHL TAAKQFELFE
     RNEAQQGRQV TGKWSWLAPP LSPTLTSNYH HGYDNTVKDP NFFYKKKESN ANQCPFHH