ID   NOSR_PSEST              Reviewed;         724 AA.
AC   Q00790;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Regulatory protein NosR;
GN   Name=nosR;
OS   Pseudomonas stutzeri (Pseudomonas perfectomarina).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14405 / Zobell / Isolate MK21;
RX   PubMed=1644760; DOI=10.1128/jb.174.16.5332-5339.1992;
RA   Cuypers H., Viebrock-Sambale A., Zumft W.G.;
RT   "NosR, a membrane-bound regulatory component necessary for expression of
RT   nitrous oxide reductase in denitrifying Pseudomonas stutzeri.";
RL   J. Bacteriol. 174:5332-5339(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 657-724.
RC   STRAIN=ATCC 14405 / Zobell / Isolate MK21;
RX   PubMed=3049543; DOI=10.1128/jb.170.10.4658-4668.1988;
RA   Viebrock A., Zumft W.G.;
RT   "Molecular cloning, heterologous expression, and primary structure of the
RT   structural gene for the copper enzyme nitrous oxide reductase from
RT   denitrifying Pseudomonas stutzeri.";
RL   J. Bacteriol. 170:4658-4668(1988).
CC   -!- FUNCTION: Transcriptional activator of the nitrous-oxide reductase gene
CC       NosZ.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: To P.denitrificans NirI. {ECO:0000305}.
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DR   EMBL; X53676; CAA78380.1; -; Genomic_DNA.
DR   PIR; A42953; A42953.
DR   RefSeq; WP_003279974.1; NZ_POUM01000011.1.
DR   AlphaFoldDB; Q00790; -.
DR   STRING; 32042.PstZobell_01052; -.
DR   PRIDE; Q00790; -.
DR   eggNOG; COG0348; Bacteria.
DR   eggNOG; COG3901; Bacteria.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR007329; FMN-bd.
DR   InterPro; IPR011399; NosR.
DR   Pfam; PF12801; Fer4_5; 2.
DR   Pfam; PF04205; FMN_bind; 1.
DR   PIRSF; PIRSF036354; NosR; 1.
DR   SMART; SM00900; FMN_bind; 1.
PE   4: Predicted;
KW   Activator; Cell membrane; DNA-binding; Membrane; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..724
FT                   /note="Regulatory protein NosR"
FT                   /id="PRO_0000096954"
FT   DNA_BIND        215..234
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   724 AA;  81873 MW;  E77BE1AC8C2B1FAB CRC64;
     MASREIWSLA GTFSTWVKGF FILLILSVCA SSLQAKEYAA EQQRLDKFFP GASLSAAEGD
     YQVRTITKGD EVLGYAFQSI RVTDMPAYSG KPINMQILLD PEGVIVDAYM LEHHEPIVLI
     GIPEQKVHDF NANYSGIHVD QRVVVGRSSD KSAVTVDAVT GATVTVMVIN EIVMRAAHTV
     AVDLGLVEAG ATARPKPALV REDVFQPTSW TELVGNGAIR RMHLTRGQVD DAFKGTEAEG
     VDVAAAEQRD ETFIDLYATH LNPPTIGRNL LGERQYADLM ANLKPGEHAF AVLANGEYSF
     KGSGYVRGGI FDRVQLRQFG DTISFRDLDF IRLSDVYAEG MPEFFEMAIF TAREQYRFDP
     GSPWNLELLV RRQVGPVESI FTSFEMPYVM PEEYIERVPL TAEELAAIEE ANRPLWVNIW
     YQKSFQVGVI LVALALLTVI LFLQDKFTQH PNFLKRLRHG YLVFTVVFIG WYALGQLSVV
     NVLTFVHALV QDFRWELFLT DPVIFILWVF TAASILLWGR GVFCGWLCPF GALQELINEA
     ARKLKIPQYD LPFSVHERLW AIKYIVLLVL FGISLESMMM AEKAAEIEPF KTAITLKFDR
     QWWFVAYAVF LLVINIFTRK VYCRYVCPLG AGLAITGRFR LFDWLKRRKE CGNPCQICAN
     ECEVQAIHPD GHINHNECHY CLDCQMTYHN ENKCPPLIAK NKRARRDKKA PAAPQLIPVQ
     VVEP