ID   NOS_ORYSJ               Reviewed;         547 AA.
AC   Q6YPG5; Q0E4R0;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Putative nitric oxide synthase;
DE            EC=1.14.13.39;
GN   OrderedLocusNames=Os02g0104700, LOC_Os02g01440;
GN   ORFNames=OJ1212_C06.17, OJA1212_C06.18;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Produces nitric oxide (NO) which is a messenger molecule
CC       involved in hormonal signaling and defense responses in plant.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline
CC         + 3 NADP(+) + 2 nitric oxide; Xref=Rhea:RHEA:19897,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16480, ChEBI:CHEBI:32682, ChEBI:CHEBI:57743,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.39;
CC   -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. NOA1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU01058}.
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DR   EMBL; AP006720; BAD06278.1; -; Genomic_DNA.
DR   EMBL; AP004049; BAD07538.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF07528.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS76549.1; -; Genomic_DNA.
DR   RefSeq; XP_015626850.1; XM_015771364.1.
DR   AlphaFoldDB; Q6YPG5; -.
DR   SMR; Q6YPG5; -.
DR   STRING; 4530.OS02T0104700-01; -.
DR   PaxDb; Q6YPG5; -.
DR   PRIDE; Q6YPG5; -.
DR   EnsemblPlants; Os02t0104700-01; Os02t0104700-01; Os02g0104700.
DR   GeneID; 4328006; -.
DR   Gramene; Os02t0104700-01; Os02t0104700-01; Os02g0104700.
DR   KEGG; osa:4328006; -.
DR   eggNOG; KOG1249; Eukaryota.
DR   HOGENOM; CLU_017878_2_1_1; -.
DR   InParanoid; Q6YPG5; -.
DR   OMA; HYNEVQD; -.
DR   OrthoDB; 612803at2759; -.
DR   PlantReactome; R-OSA-5633340; Citrulline-nitric oxide cycle.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   ExpressionAtlas; Q6YPG5; baseline and differential.
DR   Genevisible; Q6YPG5; OS.
DR   GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0004517; F:nitric-oxide synthase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR030378; G_CP_dom.
DR   InterPro; IPR006073; GTP-bd.
DR   InterPro; IPR044229; NOA1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR47569; PTHR47569; 1.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51721; G_CP; 1.
PE   3: Inferred from homology;
KW   NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..547
FT                   /note="Putative nitric oxide synthase"
FT                   /id="PRO_0000213737"
FT   DOMAIN          167..343
FT                   /note="CP-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT   REGION          24..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          72..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   547 AA;  59510 MW;  41981AD9C5C8B1C9 CRC64;
     MAAPPLLSLS QRLLFLSLSL PKPQLAPNPS SFSPTRAAST APPPPEGAGP AAPSRGDRFL
     GTQLAAEAAA RVLAPEDAER RRRRREKRKA LARKPSAAAC YGCGAPLQTA DEAAPGYVHP
     ATYDLKKRHH QLRTVLCGRC KLLSHGHMIT AVGGHGGYPG GKQFVSADQL RDKLSYLRHE
     KALIIKLVDI VDFNGSFLAR VRDFAGANPI ILVITKVDLL PRDTDLNCIG DWVVEAVVKK
     KLNVLSVHLT SSKSLVGVTG VISEIQQEKK GRDVYILGSA NVGKSAFISA MLRTMAYKDP
     VAAAAQKYKP IQSAVPGTTL GPIQIEAFLG GGKLYDTPGV HLHHRQAAVI HADDLPSLAP
     QSRLRARCFP ANDTDVGLSG NSLFWGGLVR IDVVKALPRT RLTFYGPKKL KINMVPTTEA
     DEFYEREVGV TLTPPAGKEK AEGWVGLQGV RELQIKYEES DRPACDIAIS GLGWVAVEPL
     GVPSSNPDES AEEEDNESGE LHLRVHVPKP VEIFVRPPLP VGKAASQWYR YQELTEEEEE
     LRPKWHY