NOS_SQUAC
ID NOS_SQUAC Reviewed; 212 AA.
AC Q9I9M2;
DT 30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Nitric oxide synthase;
DE Short=NOS;
DE EC=1.14.13.39;
DE Flags: Fragment;
OS Squalus acanthias (Spiny dogfish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC Elasmobranchii; Squalomorphii; Squaliformes; Squalidae; Squalus.
OX NCBI_TaxID=7797;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Gill;
RA Farmerie W.G., Holland K.P., Catches J.S., Morrison-Shetlar A.I.,
RA Claiborne J.B. III, Edwards S.L., Evans D.H.;
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with
CC diverse functions throughout the body. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline
CC + 3 NADP(+) + 2 nitric oxide; Xref=Rhea:RHEA:19897,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16480, ChEBI:CHEBI:32682, ChEBI:CHEBI:57743,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.39;
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC Note=Binds 1 FAD. {ECO:0000250};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC Note=Binds 1 FMN. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the NOS family. {ECO:0000305}.
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DR EMBL; AF232227; AAF36405.2; -; mRNA.
DR AlphaFoldDB; Q9I9M2; -.
DR SMR; Q9I9M2; -.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004517; F:nitric-oxide synthase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR001094; Flavdoxin-like.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR Pfam; PF00258; Flavodoxin_1; 1.
DR PRINTS; PR00369; FLAVODOXIN.
DR SUPFAM; SSF52218; SSF52218; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 2: Evidence at transcript level;
KW Calmodulin-binding; FAD; Flavoprotein; FMN; Heme; Iron; Metal-binding;
KW NADP; Oxidoreductase.
FT CHAIN <1..>212
FT /note="Nitric oxide synthase"
FT /id="PRO_0000170948"
FT DOMAIN 60..>212
FT /note="Flavodoxin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT REGION 30..50
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000255"
FT REGION 155..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 11
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /evidence="ECO:0000250|UniProtKB:P29474"
FT BINDING 186..>212
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT NON_TER 1
FT NON_TER 212
SQ SEQUENCE 212 AA; 23651 MW; 3A6B115A679E6CFF CRC64;
LNYQLSPSFE YQSDPWFTHV WKGVNGTPTK KRAIGFKKLA KAVKFSTKLM GQAMAKRVKA
TILYATETGK SQVYAKTLCE IFKHAFDAKV MSMDEYDIVH LEHEALVLVV TSTFGNGDPP
ENGEKFGSAL MEIRHPSSNS AERKSYKVRF NSVSSYSDSR KSSSDEPEHK DNFESTGPLA
NVRFSAFGLG SRAYPHFCAF ARAVDTLLEE LG