NOT1_SCHPO
ID NOT1_SCHPO Reviewed; 2100 AA.
AC P87112;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=General negative regulator of transcription subunit 1;
GN Name=not1; ORFNames=SPAC20G8.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1341 AND SER-1568, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
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DR EMBL; CU329670; CAB08600.1; -; Genomic_DNA.
DR PIR; T38128; T38128.
DR RefSeq; NP_593323.1; NM_001018754.2.
DR AlphaFoldDB; P87112; -.
DR SMR; P87112; -.
DR BioGRID; 278485; 15.
DR STRING; 4896.SPAC20G8.06.1; -.
DR iPTMnet; P87112; -.
DR MaxQB; P87112; -.
DR PaxDb; P87112; -.
DR PRIDE; P87112; -.
DR EnsemblFungi; SPAC20G8.06.1; SPAC20G8.06.1:pep; SPAC20G8.06.
DR GeneID; 2542001; -.
DR KEGG; spo:SPAC20G8.06; -.
DR PomBase; SPAC20G8.06; not1.
DR VEuPathDB; FungiDB:SPAC20G8.06; -.
DR eggNOG; KOG1831; Eukaryota.
DR HOGENOM; CLU_000286_3_1_1; -.
DR InParanoid; P87112; -.
DR OMA; IDEYHCY; -.
DR PhylomeDB; P87112; -.
DR PRO; PR:P87112; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0030014; C:CCR4-NOT complex; IDA:PomBase.
DR GO; GO:0030015; C:CCR4-NOT core complex; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0060090; F:molecular adaptor activity; IBA:GO_Central.
DR GO; GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IBA:GO_Central.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:PomBase.
DR Gene3D; 1.25.40.840; -; 1.
DR InterPro; IPR007196; CCR4-Not_Not1_C.
DR InterPro; IPR032191; CNOT1_CAF1_bind.
DR InterPro; IPR024557; CNOT1_dom_4.
DR InterPro; IPR032194; CNOT1_HEAT.
DR InterPro; IPR032193; CNOT1_TTP_bind.
DR InterPro; IPR038535; CNOT1_TTP_bind_sf.
DR InterPro; IPR040398; Not1.
DR PANTHER; PTHR13162; PTHR13162; 1.
DR Pfam; PF16415; CNOT1_CAF1_bind; 1.
DR Pfam; PF16418; CNOT1_HEAT; 1.
DR Pfam; PF16417; CNOT1_TTP_bind; 1.
DR Pfam; PF12842; DUF3819; 1.
DR Pfam; PF04054; Not1; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..2100
FT /note="General negative regulator of transcription subunit
FT 1"
FT /id="PRO_0000318140"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1341
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 1568
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 2100 AA; 237135 MW; 760ADA97C406E1CC CRC64;
MKSQEQQTPE TREKSSSTSE SGHPISLDSK TPPPNDSKIE SIVKAQILFL LSTLREDQYD
TKLEQIRQLI NKNAPRVYHH FLRRLIQGNS YRIFGTGKSS DGLATYKLLL DELKSLTKSW
VMAKRFSDAI SGSDSEVFED FDIEAFLDHF QFSPLERTNL LIGLTTSVKP TLAKKASALL
KNTFQSLLNQ LSDPTQQQSI DKNDLDLLRE GLFTTDYDRI PLIKVPKFDA IIEKKLANSR
PLTALFGPMK ASKATLKSMK EAILKKYPPG TATETDVAYL INSLVAIHDY GAWDTVLMGK
AIVSSFENLN WEAMLSMFDN PKFMITGTPS LVLFFTIFTN AYKLRQTNFT LDFLWVLWRN
PLPQLSIISH IILSPTSMFD IREFNVNPVV SVDSLKDYSE ELVNYAKIYE KSNLNCIELV
QILLRLLSEV VTYETSLFLN FLDEKVSAEL LLLGTLQLPL AWNPVQESLA FQWCSDFFSN
FKEHKFVFVR IERTNPQFLF AFLRSLWLKD SSSVNQVVEF IIENDFTSHI GNIQPNRFAL
EIAALAAARK ALSFQDWLDK KMLEFEDADG LNVFLVEVLD FLMSRAALQK NESEQKEESV
VLSIDTVNVL LTTLMDNVSI SEEVSEHIKD VQILCLQVYP RLFSLGHDRD SIVIATNTTN
SFSPDVESEV ESYFQALYER RISIGKIVLT LQNFKKSENP RDLDLFACLQ HSLFDEYRFF
PDYPLEALAL TAVLFGSLIQ FELLSFVPLG VALRYVYQAL LMPTDSKMFR FGMQALVQFQ
EKLPKYINYC NLILGIPSLQ LVRPDIYDSI REMIASNENT EVEKDKLDSF SALANPASPK
APEYTFVSIH VPPLNVPNAE GEIEESVCDN ILFAINNLSQ LNFNEKLKDV KDNLTPAYLP
WFSHYIVTQR VSREANFLSL YGKFLEELKS SDLYKIVFRD TLQAILDIMN NNAETLSPSE
KTNLKNLGSW LGSITLLRNK PITTLQVSFK DLLLEGIDSG RIDRVLPFVC KVLEKASSSV
IFKPPNPWLM GILKLLVELY QFADFRLNLK FEIELLLNNL NVKMDNIEPS EMYRNHLVQK
ADLEKELPED VLNAEFPDGT DVITQYIVAA SSQITVTDAV AQVFGKPKPA IKNITQLIIQ
QSVLEIISAV VRRSVGIAAI TTKSLLQKDF AAESNPSRLL LAARQMAKTL AGNLAMVTCR
EPLQMLMINN FRTIALQDVE NVHAAVAQAI DELVSQNLFV ASSVIESVAS ETAIAEIDAE
IEHMIVERVR HRKTTPNLPF VDPAGAANLH LNLPSVLKLS SELTPQQFQL YENFDRLSLS
TIMSNNSFTS LNGLRTDSAD STDALNSNLN NTVENEANQT ALNYARNLLI IIGQLFQLAA
QMPYTSMQEV PADHELHELV NQFLTGVASI NHPIADHVFL LCAQECCRIL LTDSKSPFIL
EVFSAILEYI CQASTKTAIN VSLYWNFSND LEKLNLPIIL SLIRFGILTS GEVDYHVARG
VRSEQGSGPV TDFAIELLRT AVGGENPMAL PGNFVNSITS LYEISESFSG ETKQAYEQLV
ETMNKSVSPA SEILSDLDNK QQLNDQIIIV FVSWVHLLRN SATNDETKAA FVYQLHKQGI
LSEPELCIQF FRCNLEAVLV AFLEAASVNA PDYFNVDAYA SLLVNVVKYT EGSTEGTVSS
KSVLFRKIIA LIIGVFAELH NSMAEFVHQK TFFRLFSSIL SELDDAKDVL ESCFVDIYSV
ILECFLAIQP RSFPAFTFAW LSLISHSYLL PKVLLVNNDK INDLFSEILM SFLKFLDLKD
DIDKAQFKLL YNGFLRIILV LLHDFPGFLA THCYQLIPYI PLECVQLRNM VLSAFPSDLH
LPDPFAQGLK VGRLPEVTRA PLISNSVSAS LEKFASALDL EACFSSSKPA EVAKLLLEVY
SSQDTPTKLN EWANYFMLCL IRHATRDSPA KQAPQFQSKS PECVIISTMN RSCDSKCRYF
LLTAIANQLR YPSSHTYYAS CCFLYLFKSS SNNPQELLIK EQMTTVLLER IICNRPHPWG
LLITFTELLK NEDYNFWKHP YIKRNDEICR LFDSLHEHVM APSSANNVST EEATELMGQV
