ID   NOT2_XENLA              Reviewed;         233 AA.
AC   Q91770; Q0IH75;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Homeobox protein not2;
DE            Short=Xnot-2;
DE            Short=Xnot2;
GN   Name=not2 {ECO:0000303|PubMed:7916680};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAA19694.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Egg {ECO:0000269|PubMed:7916680};
RX   PubMed=7916680; DOI=10.1242/dev.119.4.991;
RA   Gont L.K., Steinbeisser H., Blumberg B., De Robertis E.M.;
RT   "Tail formation as a continuation of gastrulation: the multiple cell
RT   populations of the Xenopus tailbud derive from the late blastopore lip.";
RL   Development 119:991-1004(1993).
RN   [2] {ECO:0000312|EMBL:AAI23278.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula {ECO:0000312|EMBL:AAI23278.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=8626017; DOI=10.1006/dbio.1996.0061;
RA   Gont L.K., Fainsod A., Kim S.-H., De Robertis E.M.;
RT   "Overexpression of the homeobox gene Xnot-2 leads to notochord formation in
RT   Xenopus.";
RL   Dev. Biol. 174:174-178(1996).
RN   [4] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=11585804; DOI=10.1242/dev.128.19.3783;
RA   Yasuo H., Lemaire P.;
RT   "Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the
RT   notochord genetic programme in Xenopus gastrulae.";
RL   Development 128:3783-3793(2001).
CC   -!- FUNCTION: Transcriptional repressor (By similarity). Plays a
CC       fundamental role in notochord formation, acting within the mesodermal
CC       region. Acts downstream of gsc and upstream of chrd and foxa4-
CC       A/pintallavis. {ECO:0000250|UniProtKB:Q06615,
CC       ECO:0000269|PubMed:11585804, ECO:0000269|PubMed:8626017}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Localized to the dorsal lip of the blastopore
CC       (Spemann organizer) during early gastrulation, after which expression
CC       continues in tissues derived from the organizer. Expressed in the
CC       notochord during mid-gastrulation, the chordoneural hinge, notochord
CC       and ventral spinal cord of the tailbud at stage 22, and finally the tip
CC       of the tail in the tadpole (stage 35). {ECO:0000269|PubMed:7916680}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI23278.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L19566; AAA19694.1; -; mRNA.
DR   EMBL; BC123277; AAI23278.1; ALT_INIT; mRNA.
DR   PIR; I51438; I51438.
DR   RefSeq; NP_001081191.1; NM_001087722.1.
DR   AlphaFoldDB; Q91770; -.
DR   SMR; Q91770; -.
DR   DNASU; 397703; -.
DR   GeneID; 397703; -.
DR   KEGG; xla:397703; -.
DR   CTD; 397703; -.
DR   Xenbase; XB-GENE-6252631; not.L.
DR   OMA; DPACKHR; -.
DR   OrthoDB; 1444296at2759; -.
DR   Proteomes; UP000186698; Chromosome 1L.
DR   Bgee; 397703; Expressed in egg cell and 7 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0014028; P:notochord formation; IMP:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR000047; HTH_motif.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..233
FT                   /note="Homeobox protein not2"
FT                   /id="PRO_0000274814"
FT   DNA_BIND        135..194
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          212..233
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        218..233
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   233 AA;  26729 MW;  7EFDD5A4F2BC2156 CRC64;
     MLHSPVFPAF GHHLADYPAM PLATDLPRTP KSSFNIDSIL SRTDRPASKV SMEMPSWQPP
     SPPFHYSYGM VPYPPMWLIK PTVGYPNMAQ AQPMRMPRGE CLCPDPACKH RGLSYSHCPN
     GALNPLSWRT GPCKLKRIRT VFTPEQLERL EKEFLKQQYM VGTERVDLAS TLNLTETQVK
     VWFQNRRIKW RKQSLEQKKA KLSQFGVILA DSSDHTDDSR ETEEEEDDVD VEL