NOTK_ASPVE
ID NOTK_ASPVE Reviewed; 577 AA.
AC L7WRR4;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 1.
DT 03-AUG-2022, entry version 27.
DE RecName: Full=Efflux pump notK' {ECO:0000303|PubMed:23213353};
DE AltName: Full=Notoamide biosynthesis cluster protein K' {ECO:0000303|PubMed:23213353};
GN Name=notK' {ECO:0000303|PubMed:23213353};
OS Aspergillus versicolor.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=46472;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=NRRL 35600;
RX PubMed=23213353; DOI=10.1039/c2md20029e;
RA Li S., Anand K., Tran H., Yu F., Finefield J.M., Sunderhaus J.D.,
RA McAfoos T.J., Tsukamoto S., Williams R.M., Sherman D.H.;
RT "Comparative analysis of the biosynthetic systems for fungal
RT bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide,
RT paraherquamide and malbrancheamide pathways.";
RL Med. Chem. Commun. 3:987-996(2012).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=17304611; DOI=10.1002/anie.200604381;
RA Kato H., Yoshida T., Tokue T., Nojiri Y., Hirota H., Ohta T.,
RA Williams R.M., Tsukamoto S.;
RT "Notoamides A-D: prenylated indole alkaloids isolated from a marine-derived
RT fungus, Aspergillus sp.";
RL Angew. Chem. Int. Ed. 46:2254-2256(2007).
CC -!- FUNCTION: Efflux pump; part of the gene cluster that mediates the
CC biosynthesis of notoamide, a fungal indole alkaloid that belongs to a
CC family of natural products containing a characteristic
CC bicyclo[2.2.2]diazaoctane core. {ECO:0000305|PubMed:23213353}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- BIOTECHNOLOGY: Notoamides have been shown to exhibit antitumoral
CC activities (PubMed:17304611). Notoamides A-C show moderate cytotoxicity
CC against HeLa and L1210 cells with IC(50) values in the range of 22-52
CC mg/ml, but the IC(50) value of notoamide D is greater than 100 mg/ml
CC (PubMed:17304611). Moreover, notoamide C induces G2/M-cell cycle arrest
CC at a concentration of 6.3 mg/ml (PubMed:17304611).
CC {ECO:0000269|PubMed:17304611}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. Azg-like subfamily. {ECO:0000305}.
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DR EMBL; JQ708194; AGC83582.1; -; Genomic_DNA.
DR AlphaFoldDB; L7WRR4; -.
DR VEuPathDB; FungiDB:ASPVEDRAFT_371653; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:InterPro.
DR GO; GO:1904823; P:purine nucleobase transmembrane transport; IEA:UniProt.
DR InterPro; IPR045018; Azg-like.
DR InterPro; IPR006043; NCS2.
DR PANTHER; PTHR43337; PTHR43337; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..577
FT /note="Efflux pump notK'"
FT /id="PRO_0000448817"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 555..577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 320
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 558
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 577 AA; 61924 MW; 75DE2EB1B68FAB09 CRC64;
MGHAEWIGRT NTAVARSAVG KWFRLEGSGH PRERKGAYFF TELRAGLATF FAMAYIISVN
ANITSDTGAT CVCPAEDLET HCNNNTEYLL CKQEVNRDIV TATAAIASVA SFFLGLLANL
PVALAPGMGL NAYFAYTVVG HHGSGLIPYS LAVTAVFVEG WIFLGLTMLG IRQWLARAIP
ASIKLATGAG IGLYLTLIGL SYSAGLGLVQ GAQDSPIQLA GCASDEFDSD GLCPSYAKMR
NPTMWIGIFC GGFFTVFLMM YRVKGAVIAG ILLVSIISWP RTTPVTYFPH TTEGDSMFDF
FKKVVTFHPI QHTLVAQDWN ISSNGGQFGL ALITFLYVDI LDATGTLYSM AKFAGAMDER
TQDFEGSAMA YTVDAICISI GSLFGSPPVT AFVESGAGIS EGGKTGLTSC MTGICFFIAV
FFAPIFASIP PWATGSTLVI VGSMMMHATL EINWRYMGDA IPAFLTISVM PFTYSIADGL
IAGIISYILI NGGVWVIAKC TGGRIVPPNR DDEHEAWTWK IPGGFFPPWL VRAVHGKKDF
WRADDEASQL DLGVMPPNGS MSSGSPEQVA EKAVGKY
