ID   NOTL_ASPSM              Reviewed;         484 AA.
AC   E1ACQ7;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Notoamide biosynthesis transcriptional activator notL {ECO:0000303|PubMed:20722388};
DE   AltName: Full=Notoamide biosynthesis cluster protein L {ECO:0000303|PubMed:20722388};
GN   Name=notL {ECO:0000303|PubMed:20722388};
OS   Aspergillus sp. (strain MF297-2).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=877550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=MF297-2;
RX   PubMed=20722388; DOI=10.1021/ja1049302;
RA   Ding Y., de Wet J.R., Cavalcoli J., Li S., Greshock T.J., Miller K.A.,
RA   Finefield J.M., Sunderhaus J.D., McAfoos T.J., Tsukamoto S., Williams R.M.,
RA   Sherman D.H.;
RT   "Genome-based characterization of two prenylation steps in the assembly of
RT   the stephacidin and notoamide anticancer agents in a marine-derived
RT   Aspergillus sp.";
RL   J. Am. Chem. Soc. 132:12733-12740(2010).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=17304611; DOI=10.1002/anie.200604381;
RA   Kato H., Yoshida T., Tokue T., Nojiri Y., Hirota H., Ohta T.,
RA   Williams R.M., Tsukamoto S.;
RT   "Notoamides A-D: prenylated indole alkaloids isolated from a marine-derived
RT   fungus, Aspergillus sp.";
RL   Angew. Chem. Int. Ed. 46:2254-2256(2007).
CC   -!- FUNCTION: Transcription factor that probably regulates the expression
CC       of the gene cluster that mediates the biosynthesis of notoamide, a
CC       fungal indole alkaloid that belongs to a family of natural products
CC       containing a characteristic bicyclo[2.2.2]diazaoctane core.
CC       {ECO:0000305|PubMed:20722388}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Notoamides have been shown to exhibit antitumoral
CC       activities (PubMed:17304611). Notoamides A-C show moderate cytotoxicity
CC       against HeLa and L1210 cells with IC(50) values in the range of 22-52
CC       mg/ml, but the IC(50) value of notoamide D is greater than 100 mg/ml
CC       (PubMed:17304611). Moreover, notoamide C induces G2/M-cell cycle arrest
CC       at a concentration of 6.3 mg/ml (PubMed:17304611).
CC       {ECO:0000269|PubMed:17304611}.
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DR   EMBL; HM622670; ADM34145.1; -; Genomic_DNA.
DR   AlphaFoldDB; E1ACQ7; -.
DR   SMR; E1ACQ7; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR013700; AflR.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF08493; AflR; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..484
FT                   /note="Notoamide biosynthesis transcriptional activator
FT                   notL"
FT                   /id="PRO_0000448818"
FT   DNA_BIND        33..60
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          70..154
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          363..387
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        75..124
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        138..154
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   484 AA;  51253 MW;  AC7482A10CF5C7FE CRC64;
     MATLDTASDT ASPPRAPVVR RSALGCTKVR DSCQSCATSK IKCPKEKTSC SKCQARGIEC
     QYFFARRPGR RRENSTGHPT SCTSTSTTAN SSSSSSRSSN SSSSSSTSPP SSSSSLSSNP
     EPTSDKDLPR PRSGDGAAAN STEPSILPPA NNSVLDITPT DNVLGPYSSD LFSVLEDPSV
     FAPLPDFDSN MTDVDFSTMD YFEQPVMDGD NFTRALSDIG SLLIPETISF DLGALESDPL
     SASAVPSLAS SVPTPSTAHS VTMMRGLSAS ISCRCVSQAL DLLKALSAKS APVSPFSGPG
     AASMTTMSSV QALMGENQQY IDNVSNLLSC SSCTEDTFLL AIVSMIVLKI LERYASAARA
     QVGGGESDTG QRPATSMIPN GKDQMRPLGR IYSTGGRDSA RSVLSELHRV QKLVNLLSPK
     LKKRQEADTR AFAHVAWGRH TVSNENDKAL STLLSPDTLA QMEGDMRKSL SSLSANIINR
     LRQD