ID   NOTL_ASPVE              Reviewed;         620 AA.
AC   L7WMF4;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Notoamide biosynthesis transcriptional activator notL' {ECO:0000303|PubMed:23213353};
DE   AltName: Full=Notoamide biosynthesis cluster protein L' {ECO:0000303|PubMed:23213353};
GN   Name=notL' {ECO:0000303|PubMed:23213353};
OS   Aspergillus versicolor.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=46472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL 35600;
RX   PubMed=23213353; DOI=10.1039/c2md20029e;
RA   Li S., Anand K., Tran H., Yu F., Finefield J.M., Sunderhaus J.D.,
RA   McAfoos T.J., Tsukamoto S., Williams R.M., Sherman D.H.;
RT   "Comparative analysis of the biosynthetic systems for fungal
RT   bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide,
RT   paraherquamide and malbrancheamide pathways.";
RL   Med. Chem. Commun. 3:987-996(2012).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=17304611; DOI=10.1002/anie.200604381;
RA   Kato H., Yoshida T., Tokue T., Nojiri Y., Hirota H., Ohta T.,
RA   Williams R.M., Tsukamoto S.;
RT   "Notoamides A-D: prenylated indole alkaloids isolated from a marine-derived
RT   fungus, Aspergillus sp.";
RL   Angew. Chem. Int. Ed. 46:2254-2256(2007).
CC   -!- FUNCTION: Transcription factor that probably regulates the expression
CC       of the gene cluster that mediates the biosynthesis of notoamide, a
CC       fungal indole alkaloid that belongs to a family of natural products
CC       containing a characteristic bicyclo[2.2.2]diazaoctane core.
CC       {ECO:0000305|PubMed:23213353}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Notoamides have been shown to exhibit antitumoral
CC       activities (PubMed:17304611). Notoamides A-C show moderate cytotoxicity
CC       against HeLa and L1210 cells with IC(50) values in the range of 22-52
CC       mg/ml, but the IC(50) value of notoamide D is greater than 100 mg/ml
CC       (PubMed:17304611). Moreover, notoamide C induces G2/M-cell cycle arrest
CC       at a concentration of 6.3 mg/ml (PubMed:17304611).
CC       {ECO:0000269|PubMed:17304611}.
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DR   EMBL; JQ708194; AGC83583.1; -; Genomic_DNA.
DR   AlphaFoldDB; L7WMF4; -.
DR   SMR; L7WMF4; -.
DR   VEuPathDB; FungiDB:ASPVEDRAFT_371654; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..620
FT                   /note="Notoamide biosynthesis transcriptional activator
FT                   notL'"
FT                   /id="PRO_0000448819"
FT   DNA_BIND        33..59
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          591..620
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        599..620
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   620 AA;  68985 MW;  A4C2FB17B7A3DD4E CRC64;
     MPPSSKSRRL PPAASDSAAS DAQKRRKNVG TACSACKARK LKCTGAPPCA NCVKSRIECT
     LDETADRRRR GVLKRKIDKL EDQEDLLGRL LEFFREGNNR CTIPLLNLIR SHASPPEIRF
     YIEHQLPLSK RTQTPELIEV CREIEQRHSF EPLPKRHILD TTPNGSHDTP RLSVPAQPWT
     SIITDDGLVS RLIFLWFTWV HPFCNFIDRD RFIRDMKSGS LSASYCSPLL VNIILSDACH
     SASGLPDDLT SKRIEFYEEA ERLLDKEEGR ISLPTAQGLG VLWMCASITG RDRQAWIKGT
     QLAYSLRELS QVSCNLPSEA NRDATALATI VNSTNWGLFN VAMVHALFAR KRPIIEPPAQ
     PPSVSNQCDH GMWYSYPNKS TGVESHTSCL FTAACNLNRI AYNLGRFLFS QEKKSSARLD
     LTDGELDALR DLNEWADQLP VCLKESIADL PHVLSLHMYN HAILTVVYGF LRTRPLYLPN
     PSASTPTVRD ALMSPARAWA ASLSSARKIA HLTLVHRANW GSDRMPGATV HCIMAALFAL
     LDSVDDPANR DAFISLTAAA AAFSRRWESP IALLRNIQNI ARQRDVTLPP ETGAFFLDPD
     QPSGNSTPIK SETPEGTAIS