NOTO_XENLA
ID NOTO_XENLA Reviewed; 236 AA.
AC Q06615; Q0IHA7;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Homeobox protein notochord;
DE Short=Xnot;
GN Name=noto; Synonyms=not;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAA79629.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP INDUCTION.
RC TISSUE=Embryo {ECO:0000269|PubMed:8095482};
RX PubMed=8095482; DOI=10.1101/gad.7.3.355;
RA von Dassow G., Schmidt J.E., Kimelman D.;
RT "Induction of the Xenopus organizer: expression and regulation of Xnot, a
RT novel FGF and activin-regulated homeo box gene.";
RL Genes Dev. 7:355-366(1993).
RN [2] {ECO:0000312|EMBL:AAI23238.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:AAI23238.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP INDUCTION.
RX PubMed=10068640; DOI=10.1242/dev.126.7.1467;
RA Sun B.I., Bush S.M., Collins-Racie L.A., LaVallie E.R.,
RA DiBlasio-Smith E.A., Wolfman N.M., McCoy J.M., Sive H.L.;
RT "derriere: a TGF-beta family member required for posterior development in
RT Xenopus.";
RL Development 126:1467-1482(1999).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=11585804; DOI=10.1242/dev.128.19.3783;
RA Yasuo H., Lemaire P.;
RT "Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the
RT notochord genetic programme in Xenopus gastrulae.";
RL Development 128:3783-3793(2001).
CC -!- FUNCTION: Transcriptional repressor. Plays a fundamental role in
CC notochord formation, acting within the mesodermal region.
CC {ECO:0000269|PubMed:11585804}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed throughout the embryo during pre-gastrula
CC stages. Localized to the dorsal lip of the blastopore (Spemann
CC organizer) during early gastrulation, after which expression continues
CC in tissues derived from the organizer. Expressed in the notochord
CC during mid-gastrulation. During neurulation, expressed in the
CC notochord, archenteron roof and the prospective floor plate. Also
CC expressed in the region that will become the epiphysis, the pineal body
CC precursor. By the early tailbud stages, expression is limited to
CC posterior notochord and floor plate before becoming restricted to the
CC tip of the tail in the tadpole. {ECO:0000269|PubMed:8095482}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expression increases markedly at the mid-blastula transition (MBT) and
CC peaks at the onset of gastrulation before declining rapidly during the
CC gastrula stages. Another decrease in expression levels occurs towards
CC the end of the neurula stages, although expression is retained into the
CC late tailbud stages. {ECO:0000269|PubMed:8095482}.
CC -!- INDUCTION: By activin, derriere, fgf2/bFGF and wnt8. Repressed by
CC bmp4/dvr-4. {ECO:0000269|PubMed:10068640, ECO:0000269|PubMed:8095482}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI23238.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z19577; CAA79629.1; -; mRNA.
DR EMBL; BC123237; AAI23238.1; ALT_INIT; mRNA.
DR PIR; A46305; A46305.
DR RefSeq; NP_001081625.1; NM_001088156.1.
DR AlphaFoldDB; Q06615; -.
DR SMR; Q06615; -.
DR GeneID; 397961; -.
DR KEGG; xla:397961; -.
DR CTD; 397961; -.
DR Xenbase; XB-GENE-865187; not.S.
DR OrthoDB; 1444296at2759; -.
DR Proteomes; UP000186698; Chromosome 1S.
DR Bgee; 397961; Expressed in gastrula and 7 other tissues.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0014028; P:notochord formation; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..236
FT /note="Homeobox protein notochord"
FT /id="PRO_0000274813"
FT DNA_BIND 138..197
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 209..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..236
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 236 AA; 27215 MW; 538FF7C9FD87CAD3 CRC64;
MLHSPVFPAF GHHLAQHPAM PLAMELPRTP KASFNIDSIL SRTDRPASKL SMEMPSWQPP
SPPSMPYRYS YGMMPYPPVW LIKPTVGYPN MAQQQPMRMP RGECLCPDPT CKERVLPFSH
CPNGAMNPLS WRTGPCKMKR IRTVFTPEQL EKLEKEFLKQ QYMVGTERVD LASTLNLTET
QVKVWFQNRR IKWRKQSLEQ KKAKLSQFGV IPADSSDHTD DSRETEEDED DLDVEL
