ID   NOTQ_ASPVE              Reviewed;         506 AA.
AC   L7WMF9;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Notoamide biosynthesis cluster transcriptional coactivator notQ' {ECO:0000303|PubMed:23213353};
DE   AltName: Full=Notoamide biosynthesis cluster protein Q' {ECO:0000303|PubMed:23213353};
GN   Name=notQ' {ECO:0000303|PubMed:23213353};
OS   Aspergillus versicolor.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=46472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL 35600;
RX   PubMed=23213353; DOI=10.1039/c2md20029e;
RA   Li S., Anand K., Tran H., Yu F., Finefield J.M., Sunderhaus J.D.,
RA   McAfoos T.J., Tsukamoto S., Williams R.M., Sherman D.H.;
RT   "Comparative analysis of the biosynthetic systems for fungal
RT   bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide,
RT   paraherquamide and malbrancheamide pathways.";
RL   Med. Chem. Commun. 3:987-996(2012).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=17304611; DOI=10.1002/anie.200604381;
RA   Kato H., Yoshida T., Tokue T., Nojiri Y., Hirota H., Ohta T.,
RA   Williams R.M., Tsukamoto S.;
RT   "Notoamides A-D: prenylated indole alkaloids isolated from a marine-derived
RT   fungus, Aspergillus sp.";
RL   Angew. Chem. Int. Ed. 46:2254-2256(2007).
RN   [3]
RP   FUNCTION.
RX   PubMed=22660767; DOI=10.1007/s00253-012-4130-0;
RA   Yin S., Yu X., Wang Q., Liu X.Q., Li S.M.;
RT   "Identification of a brevianamide F reverse prenyltransferase BrePT from
RT   Aspergillus versicolor with a broad substrate specificity towards
RT   tryptophan-containing cyclic dipeptides.";
RL   Appl. Microbiol. Biotechnol. 97:1649-1660(2013).
CC   -!- FUNCTION: Transcription factor that probably regulates the expression
CC       of the gene cluster that mediates the biosynthesis of notoamide, a
CC       fungal indole alkaloid that belongs to a family of natural products
CC       containing a characteristic bicyclo[2.2.2]diazaoctane core.
CC       {ECO:0000305|PubMed:23213353}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Notoamides have been shown to exhibit antitumoral
CC       activities (PubMed:17304611). Notoamides A-C show moderate cytotoxicity
CC       against HeLa and L1210 cells with IC(50) values in the range of 22-52
CC       mg/ml, but the IC(50) value of notoamide D is greater than 100 mg/ml
CC       (PubMed:17304611). Moreover, notoamide C induces G2/M-cell cycle arrest
CC       at a concentration of 6.3 mg/ml (PubMed:17304611).
CC       {ECO:0000269|PubMed:17304611}.
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DR   EMBL; JQ708194; AGC83588.1; -; Genomic_DNA.
DR   AlphaFoldDB; L7WMF9; -.
DR   SMR; L7WMF9; -.
DR   VEuPathDB; FungiDB:ASPVEDRAFT_76488; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..506
FT                   /note="Notoamide biosynthesis cluster transcriptional
FT                   coactivator notQ'"
FT                   /id="PRO_0000448820"
FT   DNA_BIND        11..38
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          73..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        73..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   506 AA;  56882 MW;  8FAE6FEFEE05BA45 CRC64;
     MPPESERKLA CLVCRHRKVA CDRGRPQCGL CRKNGFDCEY KTREHRPGLR AGYVSQLEKR
     VEDLERQMED VLGRLSQSQP SQPATERDDL ATTPSTGRLA PPLCVQNVES TNSPQADTAP
     VETTALTYEL QLVWLQKYQP WFPIMHHTSA VKAVAIWDSP GIPWERKQFL SERLRQEVIV
     NAMGSLHLRT VQALLTLSIL FWGEGKWVQY SNLAAMCKRL SQQLGLPTVA GVAAAQPSKM
     CSLDAVLSDT KIDHEERLRA FWMIEMLDSI FALGLPSYPP TTAAPLGAIL PCSDTAWALQ
     DHFGECVPFH DLQYSSGFSM CISLCTVELA PVHQFQHSVI QTGAMAGGPE WQSAAQRLDE
     RLTIWREEFV AAVFRLINAE CPHDARAEMR PAVITLLQSQ TSLPEGVGQE TEPWPYANHR
     CMYACENMAA KIRRMEESEL ESCSPCLVLP IFAAARFYIA VAEHRTPALQ SALPIEFYDL
     RYAVAEICHL LQRWVDRYSS PVLRSV