ID   NOTR_ASPSM              Reviewed;         460 AA.
AC   E1ACR3;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 23.
DE   RecName: Full=Notoamide biosynthesis cluster transcriptional coactivator notR {ECO:0000303|PubMed:20722388};
DE   AltName: Full=Notoamide biosynthesis cluster protein R {ECO:0000303|PubMed:23213353};
OS   Aspergillus sp. (strain MF297-2).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=877550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MF297-2;
RX   PubMed=20722388; DOI=10.1021/ja1049302;
RA   Ding Y., de Wet J.R., Cavalcoli J., Li S., Greshock T.J., Miller K.A.,
RA   Finefield J.M., Sunderhaus J.D., McAfoos T.J., Tsukamoto S., Williams R.M.,
RA   Sherman D.H.;
RT   "Genome-based characterization of two prenylation steps in the assembly of
RT   the stephacidin and notoamide anticancer agents in a marine-derived
RT   Aspergillus sp.";
RL   J. Am. Chem. Soc. 132:12733-12740(2010).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=17304611; DOI=10.1002/anie.200604381;
RA   Kato H., Yoshida T., Tokue T., Nojiri Y., Hirota H., Ohta T.,
RA   Williams R.M., Tsukamoto S.;
RT   "Notoamides A-D: prenylated indole alkaloids isolated from a marine-derived
RT   fungus, Aspergillus sp.";
RL   Angew. Chem. Int. Ed. 46:2254-2256(2007).
RN   [3]
RP   FUNCTION.
RX   PubMed=22188465; DOI=10.1021/ja2093212;
RA   Li S., Finefield J.M., Sunderhaus J.D., McAfoos T.J., Williams R.M.,
RA   Sherman D.H.;
RT   "Biochemical characterization of NotB as an FAD-dependent oxidase in the
RT   biosynthesis of notoamide indole alkaloids.";
RL   J. Am. Chem. Soc. 134:788-791(2012).
RN   [4]
RP   FUNCTION.
RX   PubMed=23213353; DOI=10.1039/c2md20029e;
RA   Li S., Anand K., Tran H., Yu F., Finefield J.M., Sunderhaus J.D.,
RA   McAfoos T.J., Tsukamoto S., Williams R.M., Sherman D.H.;
RT   "Comparative analysis of the biosynthetic systems for fungal
RT   bicyclo[2.2.2]diazaoctane indole alkaloids: the (+)/(-)-notoamide,
RT   paraherquamide and malbrancheamide pathways.";
RL   Med. Chem. Commun. 3:987-996(2012).
CC   -!- FUNCTION: Transcription factor that probably regulates the expression
CC       of the gene cluster that mediates the biosynthesis of notoamide, a
CC       fungal indole alkaloid that belongs to a family of natural products
CC       containing a characteristic bicyclo[2.2.2]diazaoctane core.
CC       {ECO:0000305|PubMed:23213353}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- BIOTECHNOLOGY: Notoamides have been shown to exhibit antitumoral
CC       activities (PubMed:17304611). Notoamides A-C show moderate cytotoxicity
CC       against HeLa and L1210 cells with IC(50) values in the range of 22-52
CC       mg/ml, but the IC(50) value of notoamide D is greater than 100 mg/ml
CC       (PubMed:17304611). Moreover, notoamide C induces G2/M-cell cycle arrest
CC       at a concentration of 6.3 mg/ml (PubMed:17304611).
CC       {ECO:0000269|PubMed:17304611}.
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DR   EMBL; HM622670; ADM34151.1; -; Genomic_DNA.
DR   AlphaFoldDB; E1ACR3; -.
DR   SMR; E1ACR3; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..460
FT                   /note="Notoamide biosynthesis cluster transcriptional
FT                   coactivator notR"
FT                   /id="PRO_0000448821"
FT   DOMAIN          74..145
FT                   /note="HTH iclR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   DNA_BIND        107..126
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   REGION          300..320
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        302..320
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   460 AA;  50244 MW;  52237E56FCFE6BD4 CRC64;
     MPGIAMDGLS KLEAESERLT ATIKSYVRHR RDVESSDQLR ASPDANMEAS RTKSVILASI
     ANIKALLGGP VELLQDLARQ VEIVACLRWL AEYQILACIP AEGGMSIQDL ADLAGVPDIQ
     LRRVIRLVGT SGFLQEPMPN YVSHTPLSAQ FVTNQSWLDA AVFMAELAAP TALHMPAATH
     RFGGSRHPTE TAYNLALNSL QPFGAAIQER PKLGRQWSAY LHHAVGLHQE KKIADMLSQL
     KWSSLGNSFV VEVGAQSTSM AHHLANKFPT LHLIVQIDRS RASRLNPDYL WPGEMMNGLT
     RDFTPQPESS PRPGSASSRV TVTYRSASMP QPVVDAAVYI LHVPVLSTNS PAGADAMETV
     KTELQDYLGI LRATAGILLI PTANLLPEPG SLSDPNFEAV ARTRDLNYLQ LANEGEMEMT
     DLFSAIETTR DGLGRLVITN QLRSHIGLTV CLTLKHETYC