NOTU2_DANRE
ID NOTU2_DANRE Reviewed; 495 AA.
AC E7F0Z8;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Carboxylesterase notum2 {ECO:0000250|UniProtKB:Q6P988};
DE EC=3.1.1.- {ECO:0000250|UniProtKB:Q6P988};
DE Flags: Precursor;
GN Name=notum2 {ECO:0000312|ZFIN:ZDB-GENE-120215-77};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23365253; DOI=10.1523/jneurosci.3694-12.2013;
RA Cantu J.A., Flowers G.P., Topczewski J.;
RT "Notum homolog plays a novel role in primary motor innervation.";
RL J. Neurosci. 33:2177-2187(2013).
CC -!- FUNCTION: Carboxylesterase that mediates deacylation of target proteins
CC (By similarity). Acts as a regulator of growth cone migration. Does not
CC act as an inhibitor of Wnt signaling (PubMed:23365253).
CC {ECO:0000250|UniProtKB:Q6P988, ECO:0000269|PubMed:23365253}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the muscle pioneers (MPs)
CC during primary motor innervation. {ECO:0000269|PubMed:23365253}.
CC -!- SIMILARITY: Belongs to the pectinacetylesterase family. Notum
CC subfamily. {ECO:0000305}.
CC -!- CAUTION: In contrast to other members of the family, it apparently does
CC not inhibit the Wnt signaling pathway, suggesting that it does not
CC mediate depalmitoleoylation of WNT proteins (PubMed:23365253). However,
CC additional evidence is required to confirm these data.
CC {ECO:0000269|PubMed:23365253, ECO:0000305}.
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DR EMBL; BX546447; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX548046; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_694400.2; XM_689308.7.
DR AlphaFoldDB; E7F0Z8; -.
DR SMR; E7F0Z8; -.
DR STRING; 7955.ENSDARP00000125786; -.
DR ESTHER; danre-e7f0z8; Pectinacetylesterase-Notum.
DR PaxDb; E7F0Z8; -.
DR Ensembl; ENSDART00000151102; ENSDARP00000125786; ENSDARG00000088411.
DR Ensembl; ENSDART00000181583; ENSDARP00000153752; ENSDARG00000088411.
DR GeneID; 566040; -.
DR CTD; 566040; -.
DR ZFIN; ZDB-GENE-120215-77; notum2.
DR eggNOG; KOG4287; Eukaryota.
DR GeneTree; ENSGT00940000165776; -.
DR HOGENOM; CLU_026533_1_1_1; -.
DR InParanoid; E7F0Z8; -.
DR OMA; TKSNWMS; -.
DR OrthoDB; 610784at2759; -.
DR PhylomeDB; E7F0Z8; -.
DR TreeFam; TF324830; -.
DR PRO; PR:E7F0Z8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000088411; Expressed in zone of skin and 8 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:1990699; F:palmitoleyl hydrolase activity; ISS:UniProtKB.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:ZFIN.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB.
DR GO; GO:1990697; P:protein depalmitoleylation; ISS:UniProtKB.
DR InterPro; IPR004963; PAE/NOTUM.
DR PANTHER; PTHR21562; PTHR21562; 1.
DR Pfam; PF03283; PAE; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Reference proteome; Secreted; Serine esterase;
KW Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..495
FT /note="Carboxylesterase notum2"
FT /id="PRO_0000432993"
FT REGION 24..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 234
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 341
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 390
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT CARBOHYD 397
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 495 AA; 54411 MW; C3D937773F0FE359 CRC64;
MNIFCHALFL LLLGVVSCQN NNRNVKPGGK PAKKPNNPAI EATQHGPEAD PNSGLAGAGD
SSKETNPGGR GSSQQSASVN KPADDMKLHF LKNTAVTCND GTAAGFYLKE FKGSKRWLIF
LEGGWCCYSK ETCDSRYKTI PRLMGSTDWP QTRRGSGLLS AQVDENPHWY NANIVFVPYC
SSDVWSGNKA ASKPKQGKET EYAFMGSQII REVIKDLVPK GLKQAKVVML AGTSAGGTGV
LLNIDKVSSL LEQQGAEAQV RGLVDSGWFL ESKQQKVPDC PDSASCTPAD AIKKGLRLWN
GVVPEKCKQQ YKRGEDWHCF FGHKLYSYIS APLFVVQWLF DEEQLRVENI YMGSQSLSEQ
QWTYMQNLGK ELKNSLKDVT AVFAPSCLSH TLITKSNWTD FQIKGTSLSR ALQCWDRSFQ
EANKNSKTAL KGCPFHLIDN CQWPQCNPTC PALIDQATQQ EMTLLQVLAS MGLDLQKLGL
DVRGDISAGM VSNGG
