ID   NOTU2_XENLA             Reviewed;         486 AA.
AC   A0A0D3QS97;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Palmitoleoyl-protein carboxylesterase notum2 {ECO:0000250|UniProtKB:Q6P988};
DE            EC=3.1.1.98 {ECO:0000250|UniProtKB:Q6P988};
DE   Flags: Precursor;
GN   Name=notum2 {ECO:0000303|PubMed:25771893};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=25771893; DOI=10.1016/j.devcel.2015.02.014;
RA   Zhang X., Cheong S.M., Amado N.G., Reis A.H., MacDonald B.T., Zebisch M.,
RA   Jones E.Y., Abreu J.G., He X.;
RT   "Notum is required for neural and head induction via Wnt deacylation,
RT   oxidation, and inactivation.";
RL   Dev. Cell 32:719-730(2015).
CC   -!- FUNCTION: Carboxylesterase that acts as a key negative regulator of the
CC       Wnt signaling pathway by specifically mediating depalmitoleylation of
CC       WNT proteins. Serine palmitoleylation of WNT proteins is required for
CC       efficient binding to frizzled receptors.
CC       {ECO:0000250|UniProtKB:Q6P988}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + H2O = (9Z)-
CC         hexadecenoate + [Wnt protein]-L-serine + H(+); Xref=Rhea:RHEA:45340,
CC         Rhea:RHEA-COMP:11170, Rhea:RHEA-COMP:11171, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:32372,
CC         ChEBI:CHEBI:85189; EC=3.1.1.98;
CC         Evidence={ECO:0000250|UniProtKB:Q6P988};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VUX3}.
CC   -!- SIMILARITY: Belongs to the pectinacetylesterase family. Notum
CC       subfamily. {ECO:0000305}.
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DR   EMBL; KP781857; AJQ30103.1; -; mRNA.
DR   RefSeq; XP_018091827.1; XM_018236338.1.
DR   AlphaFoldDB; A0A0D3QS97; -.
DR   SMR; A0A0D3QS97; -.
DR   GeneID; 108701542; -.
DR   KEGG; xla:108701542; -.
DR   OrthoDB; 610784at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 108701542; Expressed in spleen and 6 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR004963; PAE/NOTUM.
DR   PANTHER; PTHR21562; PTHR21562; 1.
DR   Pfam; PF03283; PAE; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Reference proteome; Secreted; Serine esterase;
KW   Signal; Wnt signaling pathway.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..486
FT                   /note="Palmitoleoyl-protein carboxylesterase notum2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000433429"
FT   ACT_SITE        223
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P988"
FT   ACT_SITE        331
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P988"
FT   ACT_SITE        380
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P988"
FT   CARBOHYD        183
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   486 AA;  55452 MW;  FBEC1C6CB22B2453 CRC64;
     MRILEIFAIL LILKEVRPQT KRSHKVNMKT PFQKRKNHHL NTDSRATLMS PADLHSVEHS
     IVQIRDPTLI PHYDQKSQED MKLHFLKNTL VTCNDGTTAG YYLRETKGSK RWIIFLEGGW
     CCYSKETCGV RYDNVKRLMS SSNWPQTRKG TGILSPRQDE NPYWWNVNAV FVPYCSSDVW
     SGNVSKTQDG YAFMGSVIIQ EVIRDLVPRG MKQAKSVILA GSSAGGTGVL INIDRVAALV
     EETTSESVQV RGLVDSGWFL DSRHSKQSDC LDISKCALTE AIKKGLKLWN GILPENCKQQ
     FKKGDEWRCF YGPRLFTSMK SPIFVVQWLY DQEQLRIENI QTEFQSMTEN QWNSIQNIGR
     EFKKSLREVP AVFAPACLSH TLITKSNWLE FQVKSVTLAK ALHCWDRSLQ ENRAPKGVIR
     GCPFHLVDNC QWPHCNPTCP AIYDATSGQE LSILQMFLKL RLESQRRGQE PKGDLGPLMS
     MLRNSG