ID   NOVF_STRNV              Reviewed;         362 AA.
AC   Q9L9G2;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Probable prephenate dehydrogenase NovF;
DE            EC=1.3.1.12;
DE   AltName: Full=Novobiocin biosynthesis protein F;
GN   Name=novF;
OS   Streptomyces niveus (Streptomyces spheroides).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=193462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA   Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT   "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT   spheroides NCIB 11891.";
RL   Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN   [2]
RP   REVIEW, AND POSSIBLE FUNCTION.
RX   PubMed=16868863; DOI=10.1055/s-2006-946699;
RA   Li S.M., Heide L.;
RT   "The biosynthetic gene clusters of aminocoumarin antibiotics.";
RL   Planta Med. 72:1093-1099(2006).
CC   -!- FUNCTION: Probable prephenate dehydrogenase that produces 4-
CC       hydroxyphenylpyruvate (4HPP) in the novobiocin biosynthesis pathway.
CC       Novobiocin is an aminocoumarin family antibiotic that targets bacterial
CC       DNA gyrases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + prephenate = 3-(4-hydroxyphenyl)pyruvate + CO2 +
CC         NADH; Xref=Rhea:RHEA:13869, ChEBI:CHEBI:16526, ChEBI:CHEBI:29934,
CC         ChEBI:CHEBI:36242, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.12;
CC   -!- PATHWAY: Antibiotic biosynthesis; novobiocin biosynthesis.
CC   -!- SIMILARITY: Belongs to the prephenate/arogenate dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; AF170880; AAF67499.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9L9G2; -.
DR   SMR; Q9L9G2; -.
DR   BioCyc; MetaCyc:MON-18092; -.
DR   UniPathway; UPA01035; -.
DR   GO; GO:0008977; F:prephenate dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0004665; F:prephenate dehydrogenase (NADP+) activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006571; P:tyrosine biosynthetic process; IEA:InterPro.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003099; Prephen_DH.
DR   Pfam; PF02153; PDH; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS51176; PDH_ADH; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..362
FT                   /note="Probable prephenate dehydrogenase NovF"
FT                   /id="PRO_0000423990"
FT   DOMAIN          2..283
FT                   /note="Prephenate/arogenate dehydrogenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00522"
SQ   SEQUENCE   362 AA;  37636 MW;  C579C7B45F6757C9 CRC64;
     MRTAVIIGTG MIGTSIGLAL RKQGVDSYLM DTSPVALRIA EAVGAGTAEE PPETVDLAVV
     AVPPVHVAPV IASHQSRGTA RFYVDVAGVK VSTRRELDAL GCDLATVVGG HPLVGRPGSG
     PLAARGDLFD GRPWALVPAV GTDAAALNRA LELVAACGAI PVVLDAEAHD RAIALGTLVP
     QIALTLVAAR LTEADSGALR LLGSVWSEIP QLVGVDSATS WTQVLAANAA PVVGELEKLS
     RDLASLLETL RGVADGDGSL AEPDGRLLEF IQRGIDGSNR VPGRYGIPTE TALADVDVSV
     DDRPAELARL FDDVAGAGVV MRGIDISQRP DSPDRTVTIS VTPRDAENLL HELRRRKWPA
     NS