ID   NOVG_STRNV              Reviewed;         318 AA.
AC   Q9L9G1;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Transcriptional regulator NovG;
DE   AltName: Full=Novobiocin biosynthesis protein G;
GN   Name=novG;
OS   Streptomyces niveus (Streptomyces spheroides).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=193462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA   Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT   "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT   spheroides NCIB 11891.";
RL   Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN   [2]
RP   FUNCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RX   PubMed=15942002; DOI=10.1099/mic.0.27669-0;
RA   Eustaquio A.S., Li S.M., Heide L.;
RT   "NovG, a DNA-binding protein acting as a positive regulator of novobiocin
RT   biosynthesis.";
RL   Microbiology 151:1949-1961(2005).
RN   [3]
RP   FUNCTION.
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=18568336; DOI=10.1007/s00203-008-0396-0;
RA   Dangel V., Eustaquio A.S., Gust B., Heide L.;
RT   "novE and novG act as positive regulators of novobiocin biosynthesis.";
RL   Arch. Microbiol. 190:509-519(2008).
RN   [4]
RP   FUNCTION.
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=19762445; DOI=10.1099/mic.0.032649-0;
RA   Dangel V., Harle J., Goerke C., Wolz C., Gust B., Pernodet J.L., Heide L.;
RT   "Transcriptional regulation of the novobiocin biosynthetic gene cluster.";
RL   Microbiology 155:4025-4035(2009).
CC   -!- FUNCTION: Transcription regulator that specifically activates
CC       expression of genes involved in the novobiocin biosynthesis pathway.
CC       Binds 5'-GTTCRACTG(N)(11)CRGTYGAAC-3' DNA sequence.
CC       {ECO:0000269|PubMed:15942002, ECO:0000269|PubMed:18568336,
CC       ECO:0000269|PubMed:19762445}.
CC   -!- DISRUPTION PHENOTYPE: Cells produce only 2% of the novobiocin.
CC       {ECO:0000269|PubMed:15942002}.
CC   -!- SIMILARITY: Belongs to the ParB family. {ECO:0000305}.
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DR   EMBL; AF170880; AAF67500.1; -; Genomic_DNA.
DR   RefSeq; WP_069626138.1; NZ_MDCR01000039.1.
DR   AlphaFoldDB; Q9L9G1; -.
DR   SMR; Q9L9G1; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003115; ParB/Sulfiredoxin_dom.
DR   InterPro; IPR036086; ParB/Sulfiredoxin_sf.
DR   SMART; SM00470; ParB; 1.
DR   SUPFAM; SSF110849; SSF110849; 1.
PE   1: Evidence at protein level;
KW   Activator; Antibiotic biosynthesis; DNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..318
FT                   /note="Transcriptional regulator NovG"
FT                   /id="PRO_0000423994"
FT   REGION          146..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..161
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   318 AA;  34368 MW;  BF7870424BAA180A CRC64;
     MTNSGDEEIT PASLKATRKG ERVSIGSLLP PSELVRSGES TEHIRVLAET DEDLPPIVVH
     RGTRRVVDGM HRLWAARFRG DESIEVVFVD GSPADVFVLA VELNRAHGLP LTLDERKSAA
     AQIMDSHPHW SDRKIARTTG LAASTVASLR SSSTAGTVGR RTGQDGRSRP NDGTDGRQRA
     AALLARNPNA SLREVTRAAG ISVGTASDVR ARLRRGEPAL TARQQAVMKL RPAAAQRSGP
     DYGRVLENLR KDPSLRFTDL GRRLLRLLDG SVPGSVEQIA QIADGVPEHC RTVVVDMARE
     CAAAWQHLAD QLADRDTA