ID   NOVK_STRNV              Reviewed;         244 AA.
AC   Q9L9F7;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Short-chain dehydrogenase/reductase family member NovK;
DE   AltName: Full=Novobiocin biosynthesis protein K;
GN   Name=novK;
OS   Streptomyces niveus (Streptomyces spheroides).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=193462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA   Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT   "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT   spheroides NCIB 11891.";
RL   Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN   [2]
RP   FUNCTION, SUBUNIT, AND PATHWAY.
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=16171397; DOI=10.1021/bi051297m;
RA   Pacholec M., Hillson N.J., Walsh C.T.;
RT   "NovJ/NovK catalyze benzylic oxidation of a beta-hydroxyl tyrosyl-S-
RT   pantetheinyl enzyme during aminocoumarin ring formation in novobiocin
RT   biosynthesis.";
RL   Biochemistry 44:12819-12826(2005).
CC   -!- FUNCTION: Non-catalytic subunit of the NovJ(2)K(2) heterotetramer that
CC       catalyzes the NADPH-dependent reduction of the tyrosyl moiety of L-
CC       beta-OH-Tyr-S-NovH intermediate to yield the tethered beta-ketotyrosyl-
CC       S-NovH in the novobiocin biosynthesis pathway. Novobiocin is an
CC       aminocoumarin family antibiotic that targets bacterial DNA gyrases.
CC       {ECO:0000269|PubMed:16171397}.
CC   -!- PATHWAY: Antibiotic biosynthesis; novobiocin biosynthesis.
CC       {ECO:0000269|PubMed:16171397}.
CC   -!- SUBUNIT: Heterotetramer; the NovJ(2)K(2) heterotetramer is composed of
CC       subunits of 2 NovJ and 2 subunits of NovK.
CC       {ECO:0000269|PubMed:16171397}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF170880; AAF67504.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9L9F7; -.
DR   SMR; Q9L9F7; -.
DR   KEGG; ag:AAF67504; -.
DR   BioCyc; MetaCyc:MON-18083; -.
DR   UniPathway; UPA01035; -.
DR   GO; GO:0043642; P:novobiocin biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; NADP.
FT   CHAIN           1..244
FT                   /note="Short-chain dehydrogenase/reductase family member
FT                   NovK"
FT                   /id="PRO_0000423996"
FT   BINDING         9..12
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         59..60
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         154..158
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   244 AA;  25632 MW;  21C177F9378724CD CRC64;
     MTRVAVVAGG GEHTGPAVAL RLAAGGFDVA LLGSEFTSAD KTVRRVEEYG RQCVTVRAEL
     SDARSVAVAF GRVRTALSGP AVLVTCVGPQ PLPDGLPEDE SADEQRYTAV RRALRPVFVC
     CQAGAGQLLR HRWGRIIIVT EPADADGNTW RTSRPVLDGL IGFTRSAALE LARSGTTVNL
     VAPADRAADS RAPAHRAAGD DSAGSYADGV AHVTEFLVDE RAVGITGQAI RVAARADVPL
     LRER