NOVO_STRNV
ID NOVO_STRNV Reviewed; 230 AA.
AC Q9L9F3; G5DAD0; T1NXB4;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=8-demethylnovobiocic acid C(8)-methyltransferase;
DE EC=2.1.1.284;
DE AltName: Full=Novobiocin biosynthesis protein O;
GN Name=novO;
OS Streptomyces niveus (Streptomyces spheroides).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=193462;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC 2449;
RX PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT spheroides NCIB 11891.";
RL Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC 2449;
RA Stecher H., Eiteljoerg I., Gruber-Khadjawi M.;
RT "Methyltransferase NovO - a versatile catalyst for enzymatic Friedel-Crafts
RT alkylation.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC 2449;
RX PubMed=16274243; DOI=10.1021/bi051599o;
RA Pacholec M., Tao J., Walsh C.T.;
RT "CouO and NovO: C-methyltransferases for tailoring the aminocoumarin
RT scaffold in coumermycin and novobiocin antibiotic biosynthesis.";
RL Biochemistry 44:14969-14976(2005).
CC -!- FUNCTION: C-methyltransferase that methylates 8-demethylnovobiocic acid
CC to produce novobiocic acid in the novobiocin biosynthesis pathway.
CC Novobiocin is an aminocoumarin family antibiotic that targets bacterial
CC DNA gyrases. {ECO:0000269|PubMed:16274243}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=8-desmethylnovobiocic acid + S-adenosyl-L-methionine = H(+) +
CC novobiocic acid + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36651,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73953, ChEBI:CHEBI:73954; EC=2.1.1.284;
CC Evidence={ECO:0000269|PubMed:16274243};
CC -!- PATHWAY: Antibiotic biosynthesis; novobiocin biosynthesis.
CC {ECO:0000269|PubMed:16274243}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AF170880; AAF67508.2; -; Genomic_DNA.
DR EMBL; JN606326; AEP25522.1; -; Genomic_DNA.
DR PDB; 5MGZ; X-ray; 1.90 A; A/B=1-230.
DR PDBsum; 5MGZ; -.
DR AlphaFoldDB; Q9L9F3; -.
DR SMR; Q9L9F3; -.
DR PRIDE; Q9L9F3; -.
DR KEGG; ag:AAF67508; -.
DR BioCyc; MetaCyc:MON-18055; -.
DR UniPathway; UPA01035; -.
DR GO; GO:0102526; F:8-demethylnovobiocic acid C8-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008168; F:methyltransferase activity; IDA:UniProtKB.
DR GO; GO:0032259; P:methylation; IDA:UniProtKB.
DR GO; GO:0043642; P:novobiocin biosynthetic process; IDA:UniProtKB.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF13649; Methyltransf_25; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic biosynthesis; Methyltransferase;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..230
FT /note="8-demethylnovobiocic acid C(8)-methyltransferase"
FT /id="PRO_0000424002"
FT HELIX 8..18
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 22..25
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 26..35
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 52..60
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 73..85
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 104..106
FT /evidence="ECO:0007829|PDB:5MGZ"
FT TURN 107..109
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 110..117
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 119..121
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 125..135
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 136..146
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 150..152
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 155..165
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 174..183
FT /evidence="ECO:0007829|PDB:5MGZ"
FT HELIX 188..201
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 204..212
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 214..218
FT /evidence="ECO:0007829|PDB:5MGZ"
FT STRAND 220..227
FT /evidence="ECO:0007829|PDB:5MGZ"
SQ SEQUENCE 230 AA; 25399 MW; B9EC63F45003F050 CRC64;
MKIEAITGSE AEAFHRMGSQ ASHRYDEFVD LLVGAGIADG QTVVDLCCGS GELEVILSSR
FPSLNLVGVD LSEDMVRIAR EYAAEQGKAL EFRHGDAQLL AGMEDLAGKA DLVVSRNAFH
RLTRLPAAFD TMLRLAKPGG AVLNCSFIHP SDFDESGFRA WVTFLNQRPW DSEMQIVWAL
AHHYAPRLDD YREALAQAAR ETPVSEQRVW IDDQGYGVPT VKCFARRAAA
