ID   NOVR_STRNV              Reviewed;         270 AA.
AC   Q9L9F0;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Decarboxylase NovR;
DE            EC=4.1.-.-;
DE   AltName: Full=Novobiocin biosynthesis protein R;
GN   Name=novR;
OS   Streptomyces niveus (Streptomyces spheroides).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=193462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA   Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT   "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT   spheroides NCIB 11891.";
RL   Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN   [2]
RP   CRYSTALLIZATION, AND POSSIBLE FUNCTION.
RX   PubMed=17139092; DOI=10.1107/s0907444906040169;
RA   Keller S., Pojer F., Heide L., Lawson D.M.;
RT   "Molecular replacement in the 'twilight zone': structure determination of
RT   the non-haem iron oxygenase NovR from Streptomyces spheroides through
RT   repeated density modification of a poor molecular-replacement solution.";
RL   Acta Crystallogr. D 62:1564-1570(2006).
CC   -!- FUNCTION: May mediate the 2 consecutive oxidative decarboxylation steps
CC       in the biosynthesis of the prenylated hydroxybenzoic acid moiety of
CC       novobiocin, an aminocoumarin family antibiotic that targets bacterial
CC       DNA gyrases.
CC   -!- PATHWAY: Antibiotic biosynthesis; novobiocin biosynthesis.
CC   -!- SIMILARITY: Belongs to the aldolase class II family. {ECO:0000305}.
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DR   EMBL; AF170880; AAF67511.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9L9F0; -.
DR   SMR; Q9L9F0; -.
DR   KEGG; ag:AAF67511; -.
DR   BioCyc; MetaCyc:MON-18093; -.
DR   UniPathway; UPA01035; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.225.10; -; 1.
DR   InterPro; IPR001303; Aldolase_II/adducin_N.
DR   InterPro; IPR036409; Aldolase_II/adducin_N_sf.
DR   Pfam; PF00596; Aldolase_II; 1.
DR   SMART; SM01007; Aldolase_II; 1.
DR   SUPFAM; SSF53639; SSF53639; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Lyase.
FT   CHAIN           1..270
FT                   /note="Decarboxylase NovR"
FT                   /id="PRO_0000423993"
SQ   SEQUENCE   270 AA;  29924 MW;  341AC8E6731A9496 CRC64;
     MSEALANMPG DDYFRQPPVF DTYAEHRAYL KFRHAVALRH FARLGFDQDG LAGLITVADP
     EHADTYWANP LAHPFSTITP ADLIRVDGDS AETVEGQRRV NIAAFNIHAE IHRARPDVQA
     VIHLHTVYGR AFSAFARKLP PLTQDACPFF EDHEVFDDFT GLVLAKDDGR RIAKQLRGHK
     AILLKNHGLV TVGETLDAAA WWFTLLDTCC HVQLLADAAG KPEEIPAEVA RLTGRQLGSH
     LLGWNSYQPL HEAALARDPD LATMEPALPS