ID   NOVU_STRNV              Reviewed;         420 AA.
AC   Q9L9E7;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2012, sequence version 2.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=C-methyltransferase NovU;
DE            EC=2.1.1.-;
DE   AltName: Full=Novobiocin biosynthesis protein U;
GN   Name=novU;
OS   Streptomyces niveus (Streptomyces spheroides).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=193462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=10770754; DOI=10.1128/aac.44.5.1214-1222.2000;
RA   Steffensky M., Muhlenweg A., Wang Z.X., Li S.M., Heide L.;
RT   "Identification of the novobiocin biosynthetic gene cluster of Streptomyces
RT   spheroides NCIB 11891.";
RL   Antimicrob. Agents Chemother. 44:1214-1222(2000).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=15752721; DOI=10.1016/j.abb.2005.01.012;
RA   Thuy T.T., Lee H.C., Kim C.G., Heide L., Sohng J.K.;
RT   "Functional characterizations of novWUS involved in novobiocin biosynthesis
RT   from Streptomyces spheroides.";
RL   Arch. Biochem. Biophys. 436:161-167(2005).
RN   [3]
RP   PATHWAY.
RC   STRAIN=ATCC 23965 / DSM 40292 / JCM 4252 / NBRC 12917 / NCIMB 11891 / NRRL
RC   2449;
RX   PubMed=16514445; DOI=10.1039/b515763c;
RA   Tello M., Jakimowicz P., Errey J.C., Freel Meyers C.L., Walsh C.T.,
RA   Buttner M.J., Lawson D.M., Field R.A.;
RT   "Characterisation of Streptomyces spheroides NovW and revision of its
RT   functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase.";
RL   Chem. Commun. (Camb.) 2006:1079-1081(2006).
CC   -!- FUNCTION: C-methyltransferase that acts together with NovW to catalyze
CC       the formation of dTDP-4-keto-6-deoxy-5-C-methyl-L-lyxo-hexose from
CC       dTDP-4-keto-6-deoxy-D-glucose in the novobiocin biosynthesis pathway,
CC       an aminocoumarin family antibiotic that targets bacterial DNA gyrases.
CC       {ECO:0000269|PubMed:15752721}.
CC   -!- PATHWAY: Antibiotic biosynthesis; novobiocin biosynthesis.
CC       {ECO:0000269|PubMed:15752721, ECO:0000269|PubMed:16514445}.
CC   -!- MISCELLANEOUS: It is unclear whether NovU acts before or after 3-
CC       epimerase NovW in the novobiocin biosynthesis pathway.
CC       {ECO:0000305|PubMed:16514445}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF170880; AAF67514.2; -; Genomic_DNA.
DR   RefSeq; WP_069626149.1; NZ_MDCR01000039.1.
DR   AlphaFoldDB; Q9L9E7; -.
DR   SMR; Q9L9E7; -.
DR   KEGG; ag:AAF67514; -.
DR   BioCyc; MetaCyc:MON-18089; -.
DR   UniPathway; UPA01035; -.
DR   GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0032259; P:methylation; IDA:UniProtKB.
DR   GO; GO:0043642; P:novobiocin biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   Gene3D; 6.20.50.110; -; 1.
DR   InterPro; IPR013630; Methyltransf_Zn-bd_dom_put.
DR   InterPro; IPR038576; Methyltransf_Zn-bd_dom_put_sf.
DR   InterPro; IPR013691; MeTrfase_14.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08421; Methyltransf_13; 1.
DR   Pfam; PF08484; Methyltransf_14; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..420
FT                   /note="C-methyltransferase NovU"
FT                   /id="PRO_0000424005"
SQ   SEQUENCE   420 AA;  45984 MW;  1364A4BDDC002C68 CRC64;
     MQDIVRPIDE CRVCGHDDWL DVVSFGSTPL AGNLLGDEDD AGGETLFPLD VVVCRRCWLM
     TLRHVIEPDV LFGHYRYVAS DAGSIIQHMR KLVDLCVERI GLTDGDLVVE FGSNTGAHLE
     LFRQAGPRVV GVDPARNLAG VANDRGVETI PAGFTAEVGE EIATRHGLAR LVYGRQCFAH
     IPDIHEVLNG VSALLAPDGL FFVEVPYLVE LLKNNQFDTI FHEHFSYFSL GSLCTLFESH
     GLRVVDVHTA DVHGGSIVVF AAPAAADHEV RPAVAEMLAE ERSQGIAEER TYREFADRTE
     RVRAQIRELV RGVVADGKTV AGYGAPTKGS ALLAACGLGH QEIRFCSDTT VLKQGKILPG
     SRIPIWSPEQ AAGHVPDYYL LLAWNYAPEI IDNEKEFLEN GGRFIVPIPE PRVISAESTL