NOXY2_ARATH
ID NOXY2_ARATH Reviewed; 93 AA.
AC Q6NNL9; Q9LYG7;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 17-JUN-2020, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein NONRESPONDING TO OXYLIPINS 2, mitochondrial {ECO:0000303|PubMed:17369372, ECO:0000303|PubMed:23370715};
DE Flags: Precursor;
GN Name=NOXY2 {ECO:0000303|PubMed:17369372, ECO:0000303|PubMed:23370715};
GN OrderedLocusNames=At5g11630 {ECO:0000312|Araport:AT5G11630};
GN ORFNames=T22P22.20 {ECO:0000312|EMBL:CAB87681.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=17369372; DOI=10.1105/tpc.106.046052;
RA Vellosillo T., Martinez M., Lopez M.A., Vicente J., Cascon T., Dolan L.,
RA Hamberg M., Castresana C.;
RT "Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate
RT lateral root development and defense responses through a specific signaling
RT cascade.";
RL Plant Cell 19:831-846(2007).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-63, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY PSEUDOMONAS
RP SYRINGAE; ISOXABEN AND 9-HYDROXYOCTADECATRIENOIC ACID, AND ALTERNATIVE
RP SPLICING.
RC STRAIN=cv. Columbia;
RX PubMed=23370715; DOI=10.1104/pp.112.207514;
RA Vellosillo T., Aguilera V., Marcos R., Bartsch M., Vicente J., Cascon T.,
RA Hamberg M., Castresana C.;
RT "Defense activated by 9-lipoxygenase-derived oxylipins requires specific
RT mitochondrial proteins.";
RL Plant Physiol. 161:617-627(2013).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=26417008; DOI=10.1104/pp.15.00992;
RA Marcos R., Izquierdo Y., Vellosillo T., Kulasekaran S., Cascon T.,
RA Hamberg M., Castresana C.;
RT "9-Lipoxygenase-derived oxylipins activate brassinosteroid signaling to
RT promote cell wall-based defense and limit pathogen infection.";
RL Plant Physiol. 169:2324-2334(2015).
CC -!- FUNCTION: [Isoform 1]: Essential for mitochondrial morphology,
CC functionality and distribution (PubMed:23370715). Contributes to 9-
CC lipoxygenase (9-LOX)-derived oxylipin synthesis, but not to
CC brassinosteroids (BRs) signaling (PubMed:26417008). Required for
CC waving-inducing oxylipin 9-hydroxyoctadecatrienoic acid and derivatives
CC (e.g. 9-HOT, 2-HOE, 13-HOT, 13-HOD, 13-KOD, 12,13-KHOD, 9-HOT, 9-HOD,
CC 9-KOT, 9-KOD and 9,10-KHOE)-mediated root development regulation,
CC including callose deposition, root waving and lateral roots formation
CC (PubMed:17369372, PubMed:23370715, PubMed:26417008). Involved in basal
CC plant defense toward pathogenic bacteria (e.g. Pseudomonas syringae pv
CC tomato), both in compatible (e.g. Pst DC3000) and incompatible (e.g.
CC Pst DC3000 avrRPM1) interactions, as well as against obligate
CC biotrophic pathogenic fungi (e.g. Golovinomyces cichoracearum),
CC probably via the promotion of callose deposition in the cell wall
CC (PubMed:17369372, PubMed:23370715, PubMed:26417008). Confers
CC sensitivity to the herbicide isoxaben, an herbicide inhibiting
CC cellulose synthesis and altering the cell wall (PubMed:23370715).
CC {ECO:0000269|PubMed:17369372, ECO:0000269|PubMed:23370715,
CC ECO:0000269|PubMed:26417008}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:23370715}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=NOXY2-alpha {ECO:0000303|PubMed:23370715};
CC IsoId=Q6NNL9-1; Sequence=Displayed;
CC Name=2; Synonyms=NOXY2-beta {ECO:0000303|PubMed:23370715};
CC IsoId=Q6NNL9-2; Sequence=VSP_060593;
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons, roots and flowers.
CC {ECO:0000269|PubMed:23370715}.
CC -!- DEVELOPMENTAL STAGE: First observed in vascular tissues, lateral root
CC primordia, and root meristems. {ECO:0000269|PubMed:23370715}.
CC -!- INDUCTION: Accumulates in cotyledons and roots after treatments with
CC isoxaben (an herbicide) and 9-hydroxyoctadecatrienoic acid (9-HOT)
CC (PubMed:23370715). Induced in leaves inoculated with Pseudomonas
CC syringae pv tomato (Pst) (PubMed:23370715).
CC {ECO:0000269|PubMed:23370715}.
CC -!- DISRUPTION PHENOTYPE: [Isoform 1]: Abnormal mitochondrial morphology,
CC functionality and distribution (PubMed:23370715). Reduced sensitivity
CC to isoxaben (an herbicide inhibiting cellulose synthesis and altering
CC the cell wall) and to waving-inducing oxylipins such as 9-
CC hydroxyoctadecatrienoic acid and derivatives (9-HOT, 2-HOE, 13-HOT, 13-
CC HOD, 13-KOD, 12,13-KHOD, 9-HOT, 9-HOD, 9-KOT, 9-KOD and 9,10-KHOE)
CC treatment leading to an altered root development (e.g. increased
CC numbers of lateral roots, disrupted callose accumulation and altered
CC root waving); 9-HOT is a potent inducer of root waving and an
CC endogenous modulator of lateral root formation (PubMed:17369372,
CC PubMed:23370715, PubMed:26417008). Enhanced susceptibility to
CC pathogenic bacteria (e.g. Pseudomonas syringae pv tomato) both
CC avirulent (e.g. Pst DC3000 avrRPM1) and virulent (e.g. Pst DC3000)
CC strains associated with reduced and delayed expression of salicylic
CC acid (SA)-responding pathogenesis-related genes (e.g. PR1, PR2 and PR4)
CC and of 9-HOT-responsive genes (e.g. PER71, PIF3, CYP71A12 and GLP9);
CC these phenotypes are associated with reduced callose deposition upon
CC infection (PubMed:17369372, PubMed:23370715, PubMed:26417008).
CC Increased susceptibility to the obligate biotrophic pathogenic fungus
CC Golovinomyces cichoracearum (PubMed:26417008). Defective in 9-
CC lipoxygenase (9-LOX)-derived oxylipin synthesis, but normal responses
CC to brassinosteroids (BRs) (PubMed:26417008).
CC {ECO:0000269|PubMed:17369372, ECO:0000269|PubMed:23370715,
CC ECO:0000269|PubMed:26417008}.
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DR EMBL; AL163814; CAB87681.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91703.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91704.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70780.1; -; Genomic_DNA.
DR EMBL; BT010793; AAR24160.1; -; mRNA.
DR EMBL; BT011267; AAR92303.1; -; mRNA.
DR PIR; T48522; T48522.
DR RefSeq; NP_001078571.1; NM_001085102.2. [Q6NNL9-2]
DR RefSeq; NP_001332362.1; NM_001343207.1. [Q6NNL9-1]
DR RefSeq; NP_196724.3; NM_121201.4. [Q6NNL9-1]
DR AlphaFoldDB; Q6NNL9; -.
DR STRING; 3702.AT5G11630.1; -.
DR PRIDE; Q6NNL9; -.
DR EnsemblPlants; AT5G11630.1; AT5G11630.1; AT5G11630. [Q6NNL9-1]
DR EnsemblPlants; AT5G11630.2; AT5G11630.2; AT5G11630. [Q6NNL9-2]
DR EnsemblPlants; AT5G11630.3; AT5G11630.3; AT5G11630. [Q6NNL9-1]
DR GeneID; 831035; -.
DR Gramene; AT5G11630.1; AT5G11630.1; AT5G11630. [Q6NNL9-1]
DR Gramene; AT5G11630.2; AT5G11630.2; AT5G11630. [Q6NNL9-2]
DR Gramene; AT5G11630.3; AT5G11630.3; AT5G11630. [Q6NNL9-1]
DR KEGG; ath:AT5G11630; -.
DR Araport; AT5G11630; -.
DR TAIR; locus:2181925; AT5G11630.
DR eggNOG; ENOG502S772; Eukaryota.
DR HOGENOM; CLU_146975_1_1_1; -.
DR OMA; PAINFVK; -.
DR OrthoDB; 1623861at2759; -.
DR PhylomeDB; Q6NNL9; -.
DR PRO; PR:Q6NNL9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6NNL9; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0052543; P:callose deposition in cell wall; IMP:UniProtKB.
DR GO; GO:0071433; P:cell wall repair; IMP:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0010311; P:lateral root formation; IMP:UniProtKB.
DR GO; GO:0019372; P:lipoxygenase pathway; IMP:UniProtKB.
DR GO; GO:0048311; P:mitochondrion distribution; IMP:UniProtKB.
DR GO; GO:0031407; P:oxylipin metabolic process; IMP:UniProtKB.
DR GO; GO:2000023; P:regulation of lateral root development; IMP:UniProtKB.
DR GO; GO:0010821; P:regulation of mitochondrion organization; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0009635; P:response to herbicide; IMP:UniProtKB.
DR GO; GO:0048364; P:root development; IMP:UniProtKB.
DR InterPro; IPR043459; NFD6/NOXY2-like.
DR InterPro; IPR033252; NOXY2.
DR PANTHER; PTHR33156; PTHR33156; 1.
DR PANTHER; PTHR33156:SF56; PTHR33156:SF56; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Mitochondrion; Plant defense; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..93
FT /note="Protein NONRESPONDING TO OXYLIPINS 2, mitochondrial"
FT /evidence="ECO:0000255"
FT /id="PRO_0000450247"
FT VAR_SEQ 84..93
FT /note="GMLCSANPGV -> ELGLSVPR (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:23370715"
FT /id="VSP_060593"
FT MUTAGEN 63
FT /note="A->V: In noxy2-1; altered mitochondrion morphology,
FT functionality and distribution, as well as reduced
FT sensitivity to the herbicide isoxaben and to waving-
FT inducing oxylipins (e.g. 9-HOT)."
FT /evidence="ECO:0000269|PubMed:23370715"
SQ SEQUENCE 93 AA; 9629 MW; C08543D75CD3C46B CRC64;
MASRCRSLSK PAFSAFRSAM NKPSIRPKSA SSFIGVPPSP GFSRPIGQLG SLQSLLPLYS
AVASARLTSC LGIDSQNSRS LAQGMLCSAN PGV
