NP1_HBOC1
ID NP1_HBOC1 Reviewed; 219 AA.
AC Q3YPH5; A0A068B6A8; A0A223DPG6; A0A341ZMH7; H6U986;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 02-JUN-2021, entry version 87.
DE RecName: Full=Non-structural protein NP-1;
DE Short=NP1;
GN Name=NP1; Synonyms=NP-1;
OS Primate bocaparvovirus 1 (strain Human bocavirus 1 type 1) (HBoV1) (Human
OS bocavirus type 1).
OC Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes;
OC Piccovirales; Parvoviridae; Parvovirinae; Bocaparvovirus;
OC Primate bocaparvovirus 1.
OX NCBI_TaxID=689403;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=St2 {ECO:0000312|EMBL:AAY45701.1};
RX PubMed=16118271; DOI=10.1073/pnas.0504666102;
RA Allander T., Tammi M.T., Eriksson M., Bjerkner A., Tiveljung-Lindell A.,
RA Andersson B.;
RT "Cloning of a human parvovirus by molecular screening of respiratory tract
RT samples.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:12891-12896(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KU3 {ECO:0000312|EMBL:AFC37602.1}, and
RC Salvador1 {ECO:0000312|EMBL:AFR53041.1};
RX PubMed=22956907; DOI=10.1371/journal.ppat.1002899;
RA Huang Q., Deng X., Yan Z., Cheng F., Luo Y., Shen W., Lei-Butters D.C.,
RA Chen A.Y., Li Y., Tang L., Soderlund-Venermo M., Engelhardt J.F., Qiu J.;
RT "Establishment of a reverse genetics system for studying human bocavirus in
RT human airway epithelia.";
RL PLoS Pathog. 8:E1002899-E1002899(2012).
RN [3] {ECO:0000312|EMBL:AEY82430.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=131_HP_AR07 {ECO:0000312|EMBL:AEY82435.1},
RC 132_HP_AR07 {ECO:0000312|EMBL:AEY82434.1},
RC 146_RT_AR07 {ECO:0000312|EMBL:AEY82443.1},
RC 256_HP_AR09 {ECO:0000312|EMBL:AEY82441.1},
RC 260_HP_AR09 {ECO:0000312|EMBL:AEY82440.1},
RC 307_HP_AR09 {ECO:0000312|EMBL:AEY82439.1},
RC 320_HP_AR09 {ECO:0000312|EMBL:AEY82442.1},
RC 326_HP_AR09 {ECO:0000312|EMBL:AEY82430.1},
RC 327_HP_AR09 {ECO:0000312|EMBL:AEY82438.1},
RC 359_HP_AR09 {ECO:0000312|EMBL:AEY82444.1},
RC 366_HP_AR09 {ECO:0000312|EMBL:AEY82436.1},
RC 398_HP_AR09 {ECO:0000312|EMBL:AEY82431.1},
RC 414_SF_AR09 {ECO:0000312|EMBL:AEY82437.1},
RC 462_HP_AR09 {ECO:0000312|EMBL:AEY82432.1}, and
RC 488_HP_AR09 {ECO:0000312|EMBL:AEY82433.1};
RX PubMed=22358354; DOI=10.1016/s1413-8670(12)70272-6;
RA Ghietto L.M., Camara A., Zhou Y., Pedranti M., Ferreyra S., Frey T.,
RA Camara J., Adamo M.P.;
RT "High prevalence of human bocavirus 1 in infants with lower acute
RT respiratory tract disease in Argentina, 2007 - 2009.";
RL Braz. J. Infect. Dis. 16:38-44(2012).
RN [4] {ECO:0000312|EMBL:AGR88363.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=3192_HN_12 {ECO:0000312|EMBL:AGR88363.1},
RC 3194_HN_12 {ECO:0000312|EMBL:AGR88364.1},
RC 3268_HN_12 {ECO:0000312|EMBL:AGR88365.1},
RC 3270_HN_12 {ECO:0000312|EMBL:AGR88366.1},
RC 3273_HN_12 {ECO:0000312|EMBL:AGR88367.1},
RC 3292_HN_12 {ECO:0000312|EMBL:AGR88368.1},
RC 3311_HN_12 {ECO:0000312|EMBL:AGR88369.1},
RC 3317_HN_12 {ECO:0000312|EMBL:AGR88370.1},
RC 3353_HN_12 {ECO:0000312|EMBL:AGR88371.1}, and
RC 3476_HN_12 {ECO:0000312|EMBL:AGR88372.1};
RA Ghietto L.M., Adamo M.P.;
RT "Prevalence of human bocavirus in children with lower acute respiratory
RT disease in Cordoba, Argentina 2012.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AIC76458.1, ECO:0000312|Proteomes:UP000128269}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=307AR09 {ECO:0000312|EMBL:AIC76458.1};
RA Cardozo Tomas A., Ghietto L.M., Insfran C., Adamo M.P.;
RT "Complete genome sequence of HBoV1 isolated form a child with pneumonia in
RT Cordoba, Argentina.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HBoV1s6 {ECO:0000312|EMBL:ASS83770.1};
RX PubMed=28777951; DOI=10.1016/j.virol.2017.07.034;
RA Ghietto L.M., Toigo D'Angelo A.P., Viale F.A., Adamo M.P.;
RT "Human bocavirus 1 infection of CACO-2 cell line cultures.";
RL Virology 510:273-280(2017).
RN [7] {ECO:0000312|EMBL:AVI59245.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=HBoV-1/BRA/PA-160/Brazil/2015 {ECO:0000312|EMBL:AVI59245.1}, and
RC HBoV-1/BRA/TO-142/Brazil/2014 {ECO:0000312|EMBL:AVI59249.1};
RX PubMed=29700140; DOI=10.1128/genomea.00235-18;
RA Watanabe A.S.A., Luchs A., Leal E., Milagres F.A.P., Komninakis S.V.,
RA Gill D.E., Lobato M.C.A.B.S., Brustulin R., das Chagas R.T.,
RA Abrao M.F.N.D.S., Soares C.V.D.A., Deng X., Sabino E.C., Delwart E.,
RA da Costa A.C.;
RT "Complete Genome Sequences of Six Human Bocavirus Strains from Patients
RT with Acute Gastroenteritis in the North Region of Brazil.";
RL Genome Announc. 6:0-0(2018).
RN [8]
RP SUBCELLULAR LOCATION.
RC STRAIN=KU2;
RX PubMed=20457462; DOI=10.1016/j.virol.2010.04.014;
RA Chen A.Y., Cheng F., Lou S., Luo Y., Liu Z., Delwart E., Pintel D., Qiu J.;
RT "Characterization of the gene expression profile of human bocavirus.";
RL Virology 403:145-154(2010).
RN [9]
RP FUNCTION.
RX PubMed=24161033; DOI=10.1186/1743-422x-10-315;
RA Li J., Yang Y., Dong Y., Li Y., Huang Y., Yi Q., Liu K., Li Y.;
RT "Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-
RT activation by NS1 protein.";
RL Virol. J. 10:315-315(2013).
RN [10]
RP NUCLEAR LOCALIZATION SIGNAL, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP 14-LYS--LYS-16 AND 24-ARG--ARG-26.
RX PubMed=23388199; DOI=10.1099/vir.0.047530-0;
RA Li Q., Zhang Z., Zheng Z., Ke X., Luo H., Hu Q., Wang H.;
RT "Identification and characterization of complex dual nuclear localization
RT signals in human bocavirus NP1: identification and characterization of
RT complex dual nuclear localization signals in human bocavirus NP1.";
RL J. Gen. Virol. 94:1335-1342(2013).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26912614; DOI=10.1128/jvi.02964-15;
RA Zou W., Cheng F., Shen W., Engelhardt J.F., Yan Z., Qiu J.;
RT "Nonstructural Protein NP1 of Human Bocavirus 1 Plays a Critical Role in
RT the Expression of Viral Capsid Proteins.";
RL J. Virol. 90:4658-4669(2016).
CC -!- FUNCTION: Required for the expression of the capsid proteins. Performs
CC the splicing and internal polyadenylation of the viral capsid-encoding
CC mRNA precursor, which allows its maturation and expression
CC (PubMed:26912614). Transactivates the viral promoter (PubMed:24161033).
CC {ECO:0000269|PubMed:24161033, ECO:0000269|PubMed:26912614}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:20457462,
CC ECO:0000269|PubMed:23388199}.
CC -!- DISRUPTION PHENOTYPE: Abolishes viral capsid-encoding mRNAs in the host
CC cytoplasm with concomitant loss of expression of the capsid proteins.
CC {ECO:0000269|PubMed:26912614}.
CC -!- SIMILARITY: Belongs to the Bocaparvovirus Non-structural protein NP-1
CC family. {ECO:0000305}.
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DR EMBL; DQ000496; AAY45701.1; -; Genomic_DNA.
DR EMBL; GQ925675; ACX50494.1; -; Genomic_DNA.
DR EMBL; JQ411251; AFC37602.1; -; Genomic_DNA.
DR EMBL; JQ923422; AFR53041.1; -; Genomic_DNA.
DR EMBL; JN632482; AEY82430.1; -; Genomic_DNA.
DR EMBL; JN632483; AEY82431.1; -; Genomic_DNA.
DR EMBL; JN632484; AEY82432.1; -; Genomic_DNA.
DR EMBL; JN632485; AEY82433.1; -; Genomic_DNA.
DR EMBL; JN632486; AEY82434.1; -; Genomic_DNA.
DR EMBL; JN632487; AEY82435.1; -; Genomic_DNA.
DR EMBL; JN632488; AEY82436.1; -; Genomic_DNA.
DR EMBL; JN632489; AEY82437.1; -; Genomic_DNA.
DR EMBL; JN632490; AEY82438.1; -; Genomic_DNA.
DR EMBL; JN632491; AEY82439.1; -; Genomic_DNA.
DR EMBL; JN632492; AEY82440.1; -; Genomic_DNA.
DR EMBL; JN632493; AEY82441.1; -; Genomic_DNA.
DR EMBL; JN632494; AEY82442.1; -; Genomic_DNA.
DR EMBL; JN632495; AEY82443.1; -; Genomic_DNA.
DR EMBL; JN632496; AEY82444.1; -; Genomic_DNA.
DR EMBL; KC878501; AGR88363.1; -; Genomic_DNA.
DR EMBL; KC878502; AGR88364.1; -; Genomic_DNA.
DR EMBL; KC878503; AGR88365.1; -; Genomic_DNA.
DR EMBL; KC878504; AGR88366.1; -; Genomic_DNA.
DR EMBL; KC878505; AGR88367.1; -; Genomic_DNA.
DR EMBL; KC878506; AGR88368.1; -; Genomic_DNA.
DR EMBL; KC878507; AGR88369.1; -; Genomic_DNA.
DR EMBL; KC878508; AGR88370.1; -; Genomic_DNA.
DR EMBL; KC878509; AGR88371.1; -; Genomic_DNA.
DR EMBL; KC878510; AGR88372.1; -; Genomic_DNA.
DR EMBL; KJ634207; AIC76458.1; -; Genomic_DNA.
DR EMBL; MG953829; AVI59245.1; -; Genomic_DNA.
DR EMBL; MG953830; AVI59249.1; -; Genomic_DNA.
DR EMBL; KY629423; ASS83770.1; -; Genomic_DNA.
DR RefSeq; YP_338087.1; NC_007455.1.
DR DNASU; 3711586; -.
DR GeneID; 3711586; -.
DR KEGG; vg:3711586; -.
DR Proteomes; UP000101074; Genome.
DR Proteomes; UP000114321; Genome.
DR Proteomes; UP000118311; Genome.
DR Proteomes; UP000128269; Genome.
DR Proteomes; UP000140113; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR021075; Bocavirus_NP1.
DR Pfam; PF11733; NP1-WLL; 1.
PE 1: Evidence at protein level;
KW Activator; Host nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..219
FT /note="Non-structural protein NP-1"
FT /id="PRO_0000445637"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 7..50
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:23388199"
FT COMPBIAS 55..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 14..16
FT /note="KRK->AAA: Complete loss of nuclear localization."
FT /evidence="ECO:0000269|PubMed:23388199"
FT MUTAGEN 24..26
FT /note="RKR->AAA: Complete loss of nuclear localization."
FT /evidence="ECO:0000269|PubMed:23388199"
SQ SEQUENCE 219 AA; 25709 MW; A09217EA5DA995E6 CRC64;
MSSGNMKDKH RSYKRKGSPE RGERKRHWQT THHRSRSRSP IRHSGERGSG SYHQEHPISH
LSSCTASKTS DQVMKTREST SGKKDNRTNP YTVFSQHRAS NPEAPGWCGF YWHSTRIARD
GTNSIFNEMK QQFQQLQIDN KIGWDNTREL LFNQKKTLDQ KYRNMFWHFR NNSDCERCNY
WDDVYRRHLA NVSSQTEADE ITDEEMLSAA ESMEADASN
