NP214_ARATH
ID NP214_ARATH Reviewed; 1819 AA.
AC F4I1T7; F4I1T6; Q94C88; Q9ZVV0;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Nuclear pore complex protein NUP214 {ECO:0000303|PubMed:21189294};
DE AltName: Full=Nucleoporin 214;
DE AltName: Full=Protein EMBRYO DEFECTIVE 1011 {ECO:0000303|PubMed:15266054};
DE AltName: Full=Protein LNO1 {ECO:0000303|PubMed:22898497};
DE AltName: Full=Protein LONO1 {ECO:0000303|PubMed:22898497};
GN Name=NUP214 {ECO:0000303|PubMed:21189294};
GN Synonyms=EMB1011 {ECO:0000303|PubMed:15266054},
GN LNO1 {ECO:0000303|PubMed:22898497};
GN OrderedLocusNames=At1g55540 {ECO:0000312|Araport:AT1G55540};
GN ORFNames=T5A14.5 {ECO:0000312|EMBL:AAD10642.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 988-1816 (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION.
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [5]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [6]
RP FUNCTION, INTERACTION WITH RH38, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=22898497; DOI=10.1104/pp.112.202192;
RA Braud C., Zheng W., Xiao W.;
RT "LONO1 encoding a nucleoporin is required for embryogenesis and seed
RT viability in Arabidopsis.";
RL Plant Physiol. 160:823-836(2012).
CC -!- FUNCTION: Required for normal embryogenesis and seed viability.
CC Involved in the first asymmetrical cell division of the zygote.
CC Regulates the number and planes of cell divisions required for
CC generating the normal embryo proper and suspensor, apical-basal axis,
CC cotyledons and meristem. {ECO:0000269|PubMed:15266054,
CC ECO:0000269|PubMed:22898497}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins Interacts (via N-terminus) with RH38.
CC {ECO:0000269|PubMed:22898497, ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:21189294}.
CC Nucleus, nuclear pore complex {ECO:0000305|PubMed:21189294}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4I1T7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I1T7-2; Sequence=VSP_057126;
CC -!- TISSUE SPECIFICITY: Highly expressed in reproductive tissues, such as
CC flowers and early developing seeds. Detected at low levels in
CC vegetative tissues, such as leaves, shoots, stems or roots. Expressed
CC in anthers, stigma papilla, embryo and endosperm.
CC {ECO:0000269|PubMed:22898497}.
CC -!- DOMAIN: Contains FG repeats mediating the translocation through the NPC
CC by interacting with transport factors. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous. Knockdown alleles have no
CC obvious defect in growth and development.
CC {ECO:0000269|PubMed:22898497}.
CC -!- MISCELLANEOUS: Named LONO after an Hawaiian god associated with
CC fertility, agriculture, rainfall and music.
CC {ECO:0000305|PubMed:22898497}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10642.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005223; AAD10642.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33262.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33263.1; -; Genomic_DNA.
DR EMBL; AY035068; AAK59573.2; -; mRNA.
DR PIR; H96597; H96597.
DR RefSeq; NP_001117499.2; NM_001124027.2. [F4I1T7-1]
DR RefSeq; NP_564694.2; NM_104429.4. [F4I1T7-2]
DR AlphaFoldDB; F4I1T7; -.
DR SMR; F4I1T7; -.
DR BioGRID; 27227; 1.
DR STRING; 3702.AT1G55540.2; -.
DR iPTMnet; F4I1T7; -.
DR PaxDb; F4I1T7; -.
DR PRIDE; F4I1T7; -.
DR ProteomicsDB; 250538; -. [F4I1T7-1]
DR EnsemblPlants; AT1G55540.1; AT1G55540.1; AT1G55540. [F4I1T7-2]
DR EnsemblPlants; AT1G55540.2; AT1G55540.2; AT1G55540. [F4I1T7-1]
DR GeneID; 842002; -.
DR Gramene; AT1G55540.1; AT1G55540.1; AT1G55540. [F4I1T7-2]
DR Gramene; AT1G55540.2; AT1G55540.2; AT1G55540. [F4I1T7-1]
DR KEGG; ath:AT1G55540; -.
DR Araport; AT1G55540; -.
DR TAIR; locus:2193849; AT1G55540.
DR eggNOG; ENOG502QSI5; Eukaryota.
DR InParanoid; F4I1T7; -.
DR OMA; KWVKESA; -.
DR OrthoDB; 326831at2759; -.
DR PRO; PR:F4I1T7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I1T7; baseline and differential.
DR Genevisible; F4I1T7; AT.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005643; C:nuclear pore; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006405; P:RNA export from nucleus; IBA:GO_Central.
DR GO; GO:0010070; P:zygote asymmetric cell division; IMP:TAIR.
DR InterPro; IPR044694; NUP214.
DR PANTHER; PTHR34418; PTHR34418; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Repeat; Translocation;
KW Transport.
FT CHAIN 1..1819
FT /note="Nuclear pore complex protein NUP214"
FT /id="PRO_0000431079"
FT REPEAT 533..534
FT /note="1"
FT REPEAT 550..551
FT /note="2"
FT REPEAT 586..587
FT /note="3"
FT REPEAT 1204..1205
FT /note="4"
FT REPEAT 1226..1227
FT /note="5"
FT REPEAT 1307..1308
FT /note="6"
FT REPEAT 1527..1528
FT /note="7"
FT REPEAT 1559..1560
FT /note="8"
FT REPEAT 1562..1563
FT /note="9"
FT REPEAT 1578..1579
FT /note="10"
FT REPEAT 1582..1583
FT /note="11"
FT REPEAT 1621..1622
FT /note="12"
FT REPEAT 1637..1638
FT /note="13"
FT REPEAT 1657..1658
FT /note="14"
FT REPEAT 1672..1673
FT /note="15"
FT REPEAT 1678..1679
FT /note="16"
FT REPEAT 1750..1751
FT /note="17"
FT REPEAT 1760..1761
FT /note="18"
FT REPEAT 1781..1782
FT /note="19"
FT REPEAT 1784..1785
FT /note="20"
FT REPEAT 1800..1801
FT /note="21"
FT REGION 494..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 533..1801
FT /note="21 X 2 AA repeats of F-G"
FT REGION 594..646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 693..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 974..999
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1073..1154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1319..1472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1494..1577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1612..1643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1795..1819
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 839..868
FT /evidence="ECO:0000255"
FT COMPBIAS 594..615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 626..643
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 974..990
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1073..1145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1331..1419
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1427..1472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1494..1526
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1804..1819
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1005..1007
FT /note="Missing (in isoform 2)"
FT /id="VSP_057126"
SQ SEQUENCE 1819 AA; 192930 MW; 2F5771A153DB287B CRC64;
MSRVEIEEDT EGDRISTNDY YFERIGEPIS IKEDDAQYDL ENPPSQPLAI SERHAVLFVA
HSSGFFVGRT NDVISASKNS NGNGDKVFIQ DLSLVDVPVG DVRILSLSAD DSILAVTVAA
DIHFFSVDSL LKKDAKPSFS YSPDESGFVK DFRWRRNDKH SYLVLSNTGK LFHGIDNAPP
RHVMDAVDAV EWSSKGSYIA VAQDNSLRIF SSKFNEKRCI ALSFDSWIGD SDEDCFVKVD
SIRWVRNNCI LLGCFQLIEG REENYLVQVI RSPDGKISDG STNLVALSFS DLFPCSMDDL
VPVGVGPHLL FSYIDQCKLA VTANRKSIDE HIVLLDWSSG DDKSAVSVVD IDRETFLPRI
GLQENNDDNT VMGLCIDRVS IEGTVNVRSG DDELKELQPY FVLVCLTLEG KLVMFNVASV
AGRPASSDTD LASSSDIEDA YTPLIEDDLS KQSSEKHQQL NIAVQNDQKH LNTEKFSTEQ
RLPNENIFSK EFESVKSSVS GDNNKKQEPY AEKPLQVEDA QQSMIPRLSG TSFGQLPMSL
GYDTNKFAGF GPALPVSEKL QKDIFAQSNS MHLQANVESK STAAFFGSPG LQNAILQSPQ
NTSSQPWSSG KSVSPPDFVS GPFPSMRDTQ HKQSVQSGTG YVNPPMSIKD KSVQVIETGR
VSALSNLSPL LGQNQDTNEG VEKIEPIPSI RASQLSQQVK SSFEKSASHQ QHKTPLSTGP
LRLEHNMSNQ PSNINEMARE MDTLLQSIEG PGGFKDSCAF ILKSNVEELE QGLESLAGKC
QTWKSTIHEQ QAEIQHLLDK TIQVLAKKTY MEGMYKQTAD NQYWQLWNRQ KLNPELEAKR
QHIMKLNKDL THQLIELERY FNRLELDRYN EDGGHPVARR GVPNRSAPSR RVQSLHSLHN
TMSSQLAAAE QLSECLSKQM TYLKIDSPVK KNVKQELFET IGIPYDASFS SPDAVKAKNA
SSAKNLLLSS IPASINQQSR QRQSSAMKNS DPETARRRRE SLDRVIFNWA AFEPPKTTVK
RMLLQEQQKT GMNQQTVLSE RLRSANNTQD RSLLHVKDHA SPVVSSNKGI MESFQQDTSE
AQSTPFKTRP PMPQSNSPFT ISPISASKPS FNWSGNKSSN TTSYAEESAP SQIKDTRTVS
QPGGSSFLPK RPVASTVLEQ TEKKAGEFKF SEAKANAFVE TAAGSVQRLS TTSSGSDFES
SKGFGAQFST MSSGAPASSF SSKSLFGFNS SSSIPGDKFT FPAVTAPLSG TPLDSTSTLF
TASSAPVSSS SQDPVPASIP ISSAPVPQTF SVTSTSTVSA TGFNVPFGKP LTSVKVDLNQ
AAPSTPSPSP GPTAGFTFNL PALSPSSPEM VSSSTGQSSL FPPSAPTSQV SSDQASATSS
LTDSSRLFSS TSLSSTPPIT PPDAFQSPQV STPSSAVPIT EPVSEPKKPE AQSSSILSTQ
STVDSVANAT KTQNEPLPVK SEISNPGTTV TPVSSSGFLS GFSSGTQSSL ASMAAPSFSW
PGSSQPQQLS STPAPFPASS PTSASPFGEK KDIVDTQEDE MDEEAPEASQ TTELSMGSFG
GFGLGSTPNP GAPKTNPFGG PFGNATTTTS NPFNMTVPSG ELFKPASFNF QNPQPSQPAG
FGSFSVTPSQ TPAQSGFGQP SQIGGGQQAL GSVLGSFGQS RQIGAGLPGA TFGSPTGFGG
SNPGSGLPNA PASGGFAAAG SSATGGFAAM ASAGRGFAGA SSTPTGGFAA LASGSGGFAG
AAPGGGGGGF GGLGSGTGGF GGFAPQGSSG GFAGAAGGGG FGGFGGQAQG QAGGGGFSAF
GGNSGATGKP SELFTQMRK
