NP24_SOLLC
ID NP24_SOLLC Reviewed; 247 AA.
AC P12670;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein NP24;
DE AltName: Full=Pathogenesis-related protein PR P23;
DE AltName: Full=Salt-induced protein;
DE Flags: Precursor;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Ailsa Craig;
RA Martin G.B., Jia Y.;
RT "Rapid transcript accumulation of pathogenesis-related genes during an
RT incompatible interaction in bacterial speck disease resistant tomato
RT plants.";
RL Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE OF 9-247, AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=cv. VFNT Cherry;
RX AGRICOLA=IND92000607; DOI=10.1007/BF00014946;
RA King G.J., Turner V.A., Hussey C.E. Jr., Wurtele E.S., Lee S.M.;
RT "Isolation and characterization of a tomato cDNA clone which codes for a
RT salt-induced protein.";
RL Plant Mol. Biol. 10:401-412(1988).
RN [3]
RP PARTIAL PROTEIN SEQUENCE.
RC STRAIN=cv. Rutgers; TISSUE=Leaf;
RX PubMed=1859873; DOI=10.1007/bf00015088;
RA Rodrigo I., Vera P., Frank R., Conejero V.;
RT "Identification of the viroid-induced tomato pathogenesis-related (PR)
RT protein P23 as the thaumatin-like tomato protein NP24 associated with
RT osmotic stress.";
RL Plant Mol. Biol. 16:931-934(1991).
RN [4]
RP PROTEIN SEQUENCE OF 22-46, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND VARIANTS NP24 II PHE-24 AND PHE-38.
RC STRAIN=cv. Better Boy; TISSUE=Pericarp;
RX PubMed=9115696; DOI=10.1016/s0031-9422(96)00667-x;
RA Pressey R.;
RT "Two isoforms of NP24: a thaumatin-like protein in tomato fruit.";
RL Phytochemistry 44:1241-1245(1997).
CC -!- FUNCTION: Has antifungal activity against P.betae and F.dahliae. May be
CC involved in disease resistance in tomatoes and/or have a possible role
CC in fruit development and ripening. {ECO:0000269|PubMed:9115696}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Or soluble fractions of
CC cytoplasmic organelles, except mitochondria and plastids.
CC -!- TISSUE SPECIFICITY: Highest levels of both isoforms found in the outer
CC pericarp, with smaller amounts in the inner pericarp.
CC {ECO:0000269|PubMed:9115696}.
CC -!- DEVELOPMENTAL STAGE: NP24 I is low in green tomatoes and it increased
CC substantially during ripening, with the largest increase occurring
CC between the pink and red stages. NP24 II is relatively high in green
CC tomatoes and it increased somewhat as the fruit turned pink, but not
CC during further ripening. {ECO:0000269|PubMed:9115696}.
CC -!- INDUCTION: By salt stress or by viroids.
CC -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00699}.
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DR EMBL; AF093743; AAC64171.1; -; Genomic_DNA.
DR EMBL; M21346; AAA34175.1; -; mRNA.
DR PIR; S07406; S07406.
DR PIR; S16264; S16264.
DR RefSeq; NP_001292922.1; NM_001305993.1.
DR PDB; 2I0W; X-ray; 2.50 A; A=22-228.
DR PDBsum; 2I0W; -.
DR AlphaFoldDB; P12670; -.
DR SMR; P12670; -.
DR STRING; 4081.Solyc08g080640.1.1; -.
DR Allergome; 9063; Sola l TLP.
DR PaxDb; P12670; -.
DR PRIDE; P12670; -.
DR GeneID; 543979; -.
DR KEGG; sly:543979; -.
DR eggNOG; ENOG502QV4N; Eukaryota.
DR HOGENOM; CLU_043181_5_0_1; -.
DR InParanoid; P12670; -.
DR OrthoDB; 1135904at2759; -.
DR PhylomeDB; P12670; -.
DR EvolutionaryTrace; P12670; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; P12670; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; IBA:GO_Central.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 2.60.110.10; -; 1.
DR InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR InterPro; IPR001938; Thaumatin.
DR InterPro; IPR017949; Thaumatin_CS.
DR PANTHER; PTHR31048; PTHR31048; 1.
DR Pfam; PF00314; Thaumatin; 1.
DR PIRSF; PIRSF002703; Thaumatin; 1.
DR PRINTS; PR00347; THAUMATIN.
DR SMART; SM00205; THN; 1.
DR SUPFAM; SSF49870; SSF49870; 1.
DR PROSITE; PS00316; THAUMATIN_1; 1.
DR PROSITE; PS51367; THAUMATIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antimicrobial; Cytoplasm; Direct protein sequencing;
KW Disulfide bond; Fungicide; Pathogenesis-related protein; Plant defense;
KW Reference proteome; Signal; Stress response.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:9115696"
FT CHAIN 22..247
FT /note="Protein NP24"
FT /id="PRO_0000034044"
FT DISULFID 30..225
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 72..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 87..93
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 141..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 146..196
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 154..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 168..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 178..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT VARIANT 24
FT /note="I -> F (in strain: cv. Rutgers and NP24 II)"
FT /evidence="ECO:0000269|PubMed:9115696"
FT VARIANT 38
FT /note="S -> F (in NP24 II)"
FT /evidence="ECO:0000269|PubMed:9115696"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 34..39
FT /evidence="ECO:0007829|PDB:2I0W"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 43..47
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 52..56
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 63..71
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:2I0W"
FT TURN 76..78
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 88..92
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 103..109
FT /evidence="ECO:0007829|PDB:2I0W"
FT TURN 110..113
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 114..120
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 125..127
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 129..135
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:2I0W"
FT HELIX 150..153
FT /evidence="ECO:0007829|PDB:2I0W"
FT TURN 156..158
FT /evidence="ECO:0007829|PDB:2I0W"
FT HELIX 167..170
FT /evidence="ECO:0007829|PDB:2I0W"
FT HELIX 174..177
FT /evidence="ECO:0007829|PDB:2I0W"
FT HELIX 189..195
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:2I0W"
FT TURN 207..209
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:2I0W"
FT STRAND 220..225
FT /evidence="ECO:0007829|PDB:2I0W"
SQ SEQUENCE 247 AA; 26646 MW; 942E56E2531031B4 CRC64;
MGYLTSSFVL FFLLCVTYTY AATIEVRNNC PYTVWAASTP IGGGRRLNRG QTWVINAPRG
TKMARIWGRT GCNFNAAGRG TCQTGDCGGV LQCTGWGKPP NTLAEYALDQ FSNLDFWDIS
LVDGFNIPMT FAPTKPSGGK CHAIHCTANI NGECPRALKV PGGCNNPCTT FGGQQYCCTQ
GPCGPTELSK FFKKRCPDAY SYPQDDPTST FTCPGGSTNY RVVFCPNGVA DPNFPLEMPA
STDEVAK