ID   NP3_RHOPR               Reviewed;         202 AA.
AC   Q94733;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Nitrophorin-3;
DE            Short=NP3;
DE   Flags: Precursor;
OS   Rhodnius prolixus (Triatomid bug).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Paraneoptera; Hemiptera; Heteroptera; Panheteroptera;
OC   Cimicomorpha; Reduviidae; Triatominae; Rhodnius.
OX   NCBI_TaxID=13249;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RA   Champagne D.E., Ribeiro J.M.C.;
RL   Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Heme-based protein that deliver nitric oxide gas (NO) to the
CC       victim while feeding, resulting in vasodilation and inhibition of
CC       platelet aggregation. Also binds tightly to histamine, which is
CC       released by the host to induce wound healing (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary glands.
CC   -!- SIMILARITY: Belongs to the calycin superfamily. Nitrophorin family.
CC       {ECO:0000305}.
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DR   EMBL; U70583; AAB09589.1; -; mRNA.
DR   AlphaFoldDB; Q94733; -.
DR   SMR; Q94733; -.
DR   VEuPathDB; VectorBase:RPRC000072; -.
DR   Proteomes; UP000015103; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0051381; F:histamine binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0070026; F:nitric oxide binding; IEA:InterPro.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.128.20; -; 1.
DR   InterPro; IPR012674; Calycin.
DR   InterPro; IPR023613; Nitrophorin.
DR   InterPro; IPR002351; Nitrophorin_domain.
DR   Pfam; PF02087; Nitrophorin; 1.
DR   PRINTS; PR00788; NITROPHORIN.
DR   SUPFAM; SSF50814; SSF50814; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Heme; Iron; Metal-binding; Reference proteome; Secreted;
KW   Signal; Vasoactive; Vasodilator.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..202
FT                   /note="Nitrophorin-3"
FT                   /id="PRO_0000021825"
FT   BINDING         80
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="proximal binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:Q26241"
FT   DISULFID        25..144
FT                   /evidence="ECO:0000250|UniProtKB:Q26241"
FT   DISULFID        62..193
FT                   /evidence="ECO:0000250|UniProtKB:Q26241"
SQ   SEQUENCE   202 AA;  22118 MW;  A335B629FC66C87C CRC64;
     MEPYSALLAV TILCLTSTMG VSGDCSTNIS PKKGLDKAKY FSGTWYVTHY LDKDPQVTDP
     YCSSFTPKES GGTVKEALYH FNSKKKTSFY NIGEGKLGSS GVQYTAKYNT VDKKRKEIEP
     ADPKDSYTLT VLEADDSSAL VHICLREGPK DLGDLYTVLS HQKTGEPSAT VKNAVAQAGL
     KLNDFVDTKT LSCTYDDQFT SM