NPA1P_HUMAN
ID NPA1P_HUMAN Reviewed; 2271 AA.
AC O60287; D3DSE5; Q96NX1; Q9NYQ1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 4.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Nucleolar pre-ribosomal-associated protein 1;
DE AltName: Full=Nucleolar protein 254 kDa;
DE AltName: Full=URB1 ribosome biogenesis 1 homolog;
GN Name=URB1; Synonyms=C21orf108, KIAA0539, NOP254, NPA1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10830953; DOI=10.1038/35012518;
RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT "The DNA sequence of human chromosome 21.";
RL Nature 405:311-319(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-186.
RC TISSUE=T-cell;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 24-2271, AND VARIANT LEU-1791.
RX PubMed=10773462; DOI=10.1016/s0378-1119(00)00089-5;
RA Slavov D., Hattori M., Sakaki Y., Rosenthal A., Shimizu N., Minoshima S.,
RA Kudoh J., Yaspo M.-L., Ramser J., Reinhardt R., Reimer C., Clancy K.,
RA Rynditch A., Gardiner K.;
RT "Criteria for gene identification and features of genome organization:
RT analysis of 6.5 Mb of DNA sequence from human chromosome 21.";
RL Gene 247:215-232(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 226-2271, AND VARIANT LEU-1791.
RC TISSUE=Brain;
RX PubMed=9628581; DOI=10.1093/dnares/5.1.31;
RA Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N.,
RA Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. IX. The
RT complete sequences of 100 new cDNA clones from brain which can code for
RT large proteins in vitro.";
RL DNA Res. 5:31-39(1998).
RN [6]
RP SEQUENCE REVISION.
RX PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT "Construction of expression-ready cDNA clones for KIAA genes: manual
RT curation of 330 KIAA cDNA clones.";
RL DNA Res. 9:99-106(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1297-1538.
RC TISSUE=Placenta;
RA Casadei R., Strippoli P., D'Addabbo P., Canaider S., Lenzi L., Vitale L.,
RA Giannone S., Carinci P., Zannotti M.;
RT "Homo sapiens KIAA0539 mRNA.";
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=12429849; DOI=10.1091/mbc.e02-05-0271;
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT "Functional proteomic analysis of human nucleolus.";
RL Mol. Biol. Cell 13:4100-4109(2002).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=16963496; DOI=10.1093/nar/gkl603;
RA Sekiguchi T., Hayano T., Yanagida M., Takahashi N., Nishimoto T.;
RT "NOP132 is required for proper nucleolus localization of DEAD-box RNA
RT helicase DDX47.";
RL Nucleic Acids Res. 34:4593-4608(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1143, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12429849,
CC ECO:0000269|PubMed:16963496}.
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DR EMBL; AP000266; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP000267; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP000268; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP000269; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471079; EAX09876.1; -; Genomic_DNA.
DR EMBL; CH471079; EAX09877.1; -; Genomic_DNA.
DR EMBL; CT001610; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AF231919; AAF72943.1; -; mRNA.
DR EMBL; AB011111; BAA25465.2; -; mRNA.
DR EMBL; AF432264; AAL28110.1; -; mRNA.
DR CCDS; CCDS46645.1; -.
DR PIR; T00269; T00269.
DR RefSeq; NP_055640.2; NM_014825.2.
DR AlphaFoldDB; O60287; -.
DR SMR; O60287; -.
DR BioGRID; 115207; 150.
DR IntAct; O60287; 47.
DR MINT; O60287; -.
DR STRING; 9606.ENSP00000372199; -.
DR GlyGen; O60287; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; O60287; -.
DR PhosphoSitePlus; O60287; -.
DR SwissPalm; O60287; -.
DR BioMuta; URB1; -.
DR SWISS-2DPAGE; O60287; -.
DR EPD; O60287; -.
DR jPOST; O60287; -.
DR MassIVE; O60287; -.
DR MaxQB; O60287; -.
DR PaxDb; O60287; -.
DR PeptideAtlas; O60287; -.
DR PRIDE; O60287; -.
DR ProteomicsDB; 49316; -.
DR Antibodypedia; 4954; 69 antibodies from 24 providers.
DR DNASU; 9875; -.
DR Ensembl; ENST00000382751.4; ENSP00000372199.3; ENSG00000142207.7.
DR GeneID; 9875; -.
DR KEGG; hsa:9875; -.
DR MANE-Select; ENST00000382751.4; ENSP00000372199.3; NM_014825.3; NP_055640.2.
DR UCSC; uc002ypn.3; human.
DR CTD; 9875; -.
DR GeneCards; URB1; -.
DR HGNC; HGNC:17344; URB1.
DR HPA; ENSG00000142207; Low tissue specificity.
DR MIM; 608865; gene.
DR neXtProt; NX_O60287; -.
DR OpenTargets; ENSG00000142207; -.
DR PharmGKB; PA162408676; -.
DR VEuPathDB; HostDB:ENSG00000142207; -.
DR eggNOG; KOG1791; Eukaryota.
DR GeneTree; ENSGT00390000014210; -.
DR HOGENOM; CLU_001185_0_0_1; -.
DR InParanoid; O60287; -.
DR OMA; FDLECCK; -.
DR OrthoDB; 804299at2759; -.
DR PhylomeDB; O60287; -.
DR TreeFam; TF313756; -.
DR PathwayCommons; O60287; -.
DR SignaLink; O60287; -.
DR BioGRID-ORCS; 9875; 638 hits in 1091 CRISPR screens.
DR ChiTaRS; URB1; human.
DR GenomeRNAi; 9875; -.
DR Pharos; O60287; Tbio.
DR PRO; PR:O60287; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; O60287; protein.
DR Bgee; ENSG00000142207; Expressed in endothelial cell and 141 other tissues.
DR Genevisible; O60287; HS.
DR GO; GO:0001650; C:fibrillar center; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032436; NopRA1_C.
DR InterPro; IPR021714; Npa1_N.
DR InterPro; IPR039844; URB1.
DR PANTHER; PTHR13500; PTHR13500; 1.
DR Pfam; PF16201; NopRA1; 1.
DR Pfam; PF11707; Npa1; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..2271
FT /note="Nucleolar pre-ribosomal-associated protein 1"
FT /id="PRO_0000057939"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2021..2042
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q571H0"
FT MOD_RES 1143
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT VARIANT 1791
FT /note="V -> L (in dbSNP:rs3761342)"
FT /evidence="ECO:0000269|PubMed:10773462,
FT ECO:0000269|PubMed:9628581"
FT /id="VAR_060586"
FT VARIANT 2071
FT /note="P -> R (in dbSNP:rs762225)"
FT /id="VAR_059705"
FT CONFLICT 368
FT /note="P -> S (in Ref. 5; BAA25465)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2271 AA; 254389 MW; 1217E7A724E816D8 CRC64;
MGVPKRKASG GQDGAASSAG AAKRARKEEL TGVRFKAQLK DPQGPGPGLE AFVSAAKKLP
REDVYDVVEG YIKISVECVE IFQLLSGEKR PESETMLIFQ VFEAILLRTA SDLSHFHVVG
TNIVKKLMNN HMKLICESLY ASGYRLARAC LSLMTAMVTQ GPEAARDVCS HFDLNKKTLY
TLVTKRDSKG VYDVRQAYVQ FALSFLIAGD DSTIVQVLEV KEFIPCIFSS GIKEDRISTI
NILLSTLKTK VVHNKNITKT QKVRFFTGQL LNHIASLYNW NGITDVNPEN VKVSAEEAGK
TMVRELVHNF LMDLCCSLKH GINFYDASLG TFGRGGNLTL LHFLLGLKTA ADDDLVADLV
VNILKVCPDL LNKYFKEVTF SFIPRAKSTW LNNIKLLNKI YEAQPEISRA FQTREFIPLP
RLLAMVMVTT VPLVCNKSMF TQALNLDSTS VRHTALSLIS VILKRALKTV DHCLNKEVWQ
ESGVYTAVMM EEFVQLFREA LSKILPDLNT VVWVWQSLKK QETKQDDKKG QKRSDGPPAA
CDAHQCDDAE TILLKAVLLQ VICLYQKVVP HVVMQYNFDF SKLLKGVISE QGLREEVPPI
LQHHMLKVAL ELPASKFLWL KAQEGPDAEI IGGERSVFYL LMKMFVTSSH LQLKSLTKLL
IMKILRDTGV FEHTWKELEL WLEHLENTME EDKETVIQFL ERILLTLVAN PYSYTDKASD
FVQEASMLQA TMTKQEADDM SIPISHIDDV LDMVDVLVEG SEGLDEEIGF TLSEDMILLT
FPFSAVVPAA LEARNKLLLG TGNEAAENVV TYLTAVLTDL LHTQRDPLAL CLLLQAYDKL
EPPCLVPCCQ QLSRFNRYYS LWIPEQAREA WLLQAQGSPS PPALPLASSF TALLQAAYES
QALRDEHIQV QLQATMPHLS MQQVLLAAKQ VLLYLRSTVE NFGQLGRSVG PPLLQLFLDL
LRRLVVHCEQ LDAQNQQRCE AARAEADLFL DMESVASLEL ANDQTLEEVL VAILRHPTLE
GWFLALEQQA LPPHTLSPVL VKLLATHFSA GVLQLLAASA PILQNIGQLG LLARYSEAIT
QSVLKELQNR RAGPATSPPK TPPQLEALQE LHPYMEGAQL REVTLALLSL PETHLVTQQP
TKSPGKERHL NALGKTLVQL LTCSPQDQLQ SGELLWSSEY VRGLGALLPT LAVDELDTVL
LHTLQRDPVL APAVGADLLD YCLARRTQAA LSIAALLLQE SCTHLLWFEQ WCLQAGPGLG
LQGDLDDFLP LIHVYLQCRT RSHFTRPAGV SSAVIPVLRK TLWRQLQSRL LSTDSPPASG
LYQEILAQLV PFARAKDLSV LMDRLPSLLH TPSSHKRWIV ADSISAALEG SAEELCAWRR
TLLESCVKWL IVSFSGGQQD DDNTQNQEKE MLLRLNALLH ALNEVDPGDW QKFVKKGLKF
RYQDHTFLKM LLTAVQLLYS PESSVRTKLI QLPVVYVMLM QHSLFLPTLL TSDGEESPDS
QVKEALVDLM LTVVEMCPSV CESSHFAVLL GAYGATLSVL DQKILLLLRA YEQNKLSLIN
FRVLLWGPAA VEHHKTCRSL GRSLWQQPSV GDILRLLDRD RMMQTILHFP QNRRLLPPED
TQELIFKDKS RVDLDGLYDP CFLLQLFSEL TRPEFVVDCR KFLDSNALGL TVTALSSYDP
QMRAIAYHVL AAYYSHLEGA RFQEQSQLLY LLDVVRNGIR TQDMRLTFTL ALFIAKAALQ
ILKPEEHMYL KVSNFLLSHE YLNMDKVPGF YQFFYSSDFE QKTEQKWVFG VLRQGIRDKQ
CYELCARRGI FHIILSFFHS PLCDEAAQNW ILEILQNAAQ VARSAYEIIR DYSLLTWILH
ILESKFLETP LLSNVISLLH TLWVTNLGDK AVEWESQRLC QPSSQEPAKR LALHLVNEFL
YVLIVLMKHL RPTLAPVQLT NFFGTLDSVL RYRATVIQAF RDMNRFTVNE TVLSTKDVLV
LLHKWSLIER DLKLQEDLRA AIEKAQAREL MKMLKDKNKP VMPARAKGPR GRKRRPGEAE
EMADPELMAS TLETCKGLLR SILTYWRPVI PGPDPTQEPV DSASPESDAP GPVYAAASLA
VSWVLRSVAE HPLSRAEAAG LIGWLKSHIL PHPVVVADLL KDSAVRSSIF RLYSRLCGAE
GLAGPVQEVA CLFNTVMLQL VAAQGRAGSP FHPAMEALSL SSLSEKDEAT QASAAFLVSL
YIKDIWLGAQ RPDTLLTHVR MVCEAADDAP SSEEEAIVVL CKDAASAASD A
