NPA1P_MOUSE
ID NPA1P_MOUSE Reviewed; 2274 AA.
AC Q571H0; Q9CYN4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Nucleolar pre-ribosomal-associated protein 1;
DE AltName: Full=URB1 ribosome biogenesis 1 homolog;
GN Name=Urb1; Synonyms=Kiaa0539, Npa1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1148-2274 (ISOFORM 1).
RC TISSUE=Spleen;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q571H0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q571H0-2; Sequence=VSP_028877, VSP_028878;
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DR EMBL; AK017495; BAB30774.1; -; mRNA.
DR EMBL; AC126671; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC134560; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK220219; BAD90144.1; -; mRNA.
DR RefSeq; NP_083773.1; NM_029497.1.
DR AlphaFoldDB; Q571H0; -.
DR SMR; Q571H0; -.
DR BioGRID; 228935; 2.
DR STRING; 10090.ENSMUSP00000114717; -.
DR iPTMnet; Q571H0; -.
DR PhosphoSitePlus; Q571H0; -.
DR EPD; Q571H0; -.
DR MaxQB; Q571H0; -.
DR PaxDb; Q571H0; -.
DR PeptideAtlas; Q571H0; -.
DR PRIDE; Q571H0; -.
DR ProteomicsDB; 293943; -. [Q571H0-1]
DR ProteomicsDB; 293944; -. [Q571H0-2]
DR GeneID; 207932; -.
DR KEGG; mmu:207932; -.
DR UCSC; uc007zwo.2; mouse. [Q571H0-2]
DR CTD; 9875; -.
DR MGI; MGI:2146468; Urb1.
DR eggNOG; KOG1791; Eukaryota.
DR InParanoid; Q571H0; -.
DR OrthoDB; 804299at2759; -.
DR BioGRID-ORCS; 207932; 28 hits in 75 CRISPR screens.
DR PRO; PR:Q571H0; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q571H0; protein.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032436; NopRA1_C.
DR InterPro; IPR021714; Npa1_N.
DR InterPro; IPR039844; URB1.
DR PANTHER; PTHR13500; PTHR13500; 1.
DR Pfam; PF16201; NopRA1; 1.
DR Pfam; PF11707; Npa1; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..2274
FT /note="Nucleolar pre-ribosomal-associated protein 1"
FT /id="PRO_0000307935"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2020..2042
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1141
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O60287"
FT VAR_SEQ 222..231
FT /note="EFIPCIFSSG -> GGSALSLGDV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028877"
FT VAR_SEQ 232..2274
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028878"
FT CONFLICT 26
FT /note="R -> Q (in Ref. 1; BAB30774)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2274 AA; 254614 MW; B1966E35F3697484 CRC64;
MGVPKRKASE GPSSAASPAG TVKRTRAEEF TGVRFKTLLK DAQGPGPALE AFVSAAKKLP
QEDMCDVVEG YIKISMECAE IFQLLSGEKR PESEMLLIFQ AFEAILLRTA SDLTHFHVVG
ANIVKKLLYN HMKLLCESLY ASGYRMARAC LDLMTAMVTQ GPEAARDVCS SLDLNKKALF
ALVTKRDSKG VHDVRLAYIQ FALSFLIAGD DNTIGQVLEI KEFIPCIFSS GIKEDKISTI
NILLSTLKTK VIHNKNITKT QKVRFFTGQF LNHIAALYNW NGITDVTPEK PEISAEEAGK
AMVRDLVHNF LMDLCCSRKH GISFYDASLG TSGRGGNLTL LHFLLSLKTA AGDDLVASLV
VSILKVCPDL LTKYFKEVTF SFLPRVKSTW LNNVKLLNKI YEAQPEISPA FWTREFIPLP
RLLAMVMVTT VPLVCNKIMF TQALNLDSIP VKHSALSLIS DILKRALKTV DHCLDKETWQ
DSDVYTAEMM EEFVQLFREA LGKILPDLNT VIWVWQSFKK QEIKEDHEKG KKSSSKTPAV
SKAAQHDVAE TILLKSVLLQ VICLYQQVVP HVIMQYNFDF SKLLKGIISE QGPSQEVPPI
LQHHMLKVAL ELPANKFLWL KAQEGPEAEI IGGERSVFYL LMKMFVNSNH LQLKSSTKLL
IMKILRDTGV FEHTWRELEL WLEHLDSTAE ERKEAVIQFL ERILLTLVMN PYSYTDKASE
FVQEASTLQA SMGKQDADDV SIPISHIDDV LDMVDVLVEG SEGLDEDIGF LLNEDMILLT
FPFSALVPAA LEARNKLLLG TDCEAGESIM AYMTAVLTDL LHTQRDPLAL CLLLQSYDKF
EPVSLLCGQQ LAQFHRYYSL WIPAQAQEAL PLQVSSSSGP CTLPPSSCFS TLLQTAYESQ
TLGDKSVQAQ LLAAVPSLAL QHMLRSAKQV LLYLKSTVEN FSQLGRSVGP ALLQSLLGLL
KQLVIHAERL DAQNQQKLEA ARAESDLFLD MESVASLELA TDKTIEELLL AILKHPTLET
WFLALEQKAL PPHTLSPILV KLLAAHFSAG VLQLLVASSP ILHKLGQLGL LAKYSEAITQ
SVLIELRTRT LNSTSTPKTL PQLEALRELH PYMEGVQIRE VTLALLALPE AHLLTQQGTQ
SLGKERHLSS LGKTLVQLLA SSHQDQLQSS ELLWCAEYVR GLGALLPTLA EHELDTVFLQ
TLQKDPVLAP VVPEGVLEYC LARRTQTALG IASLLLQYSG THLVKFELWC GQPGVGPTLQ
EHLDDFFPLI HVYLQHRAQG SFMRPTGVSS AVTPVLKALW RQVRDRFFHI TGPSKDALHL
EALAQLIPFA RTKDLHVLMD HLPNTLRTLS NHKSWTLADS VSAALAESAE ELGSWRKTLL
RSCIQWLAVS FSGREPEDEN TQEHEKAMLV RLSELLHAVK EVDPGDWQQF VKTGLKFRYH
DLTFLKTLLS ATKLLYGPES SGRTKLVQLS VVHMMLTQHS LFLPTMLSSE EEETPDSGVK
ETLLDLMSTV VRLCPSVCQS SHFAVLLGTY SATLSVLDQK ILLLLRAYEQ NNLSLISFRV
LLWGPAAVEH HKTCRSLGKS LWQQPSVGDI LRLLDPDRMM QTILHFPQYR KLLPTEDTGE
PLVFKDKTAR VDLDSLYDPC FLLHLFGELT RPEFVVDCRK FLDSNALGLT VAALSSYDPQ
MRAAAYYVLA AYYSHLEGAR FREQSQVLYL LDVVRNGIRT PNLRLPFTVA LFIAKAAVQI
LKPEEHMYWK ISKFLLSHEN LNMDKLPGFY QFFYSSDFQQ KTEQEWVLEI LRQGIRDKHC
YELCSRRGVF HIILSFFNSP LCDEVAQNWI LEILQNVAHI TRSAYEVIRD YSLLTWVLHI
LESRFVETQL LSNVISLLHT LWVTNLGNKA PEERSQPPGQ VGSQESEKML ALHMVSEFLY
VLIALTKHLR PTLASAQLMN FFWTLESVLS YRATIIKLFK DMGRFTVNKV ALSTKDVLIL
LHKWSLIERD TKLQGELKAV IEQHQAKDLM KMLKDKSRPV VAAQARGPRG RKRRHGGLEE
TAEPQLEASC LEKCKDLLRA TLTHWGPGDP LPGPTQGSVG QTIPKSKTLS SAHAAVSLVA
SWVLRSLAER PVSRAEVTRL LDWLKSHILP QPMVVADLLG DSAVKTGIFK LYNHHCSAQG
LVGPAQDVAC KFSTVMLQLL VAQGRKESPF HSVAEALCLD SLNEKEEAKR APAAFLVSLY
VKDMWLGAQQ PDTFLAHIRM VCEAAKDVPL DEPEAIVVLC RNVDSSAQCL TRSR
