NPAL3_HUMAN
ID NPAL3_HUMAN Reviewed; 406 AA.
AC Q6P499; A2A298; Q6MZT9; Q9BVE6;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=NIPA-like protein 3;
GN Name=NIPAL3; Synonyms=NPAL3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Endometrium;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Lymph, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
CC -!- INTERACTION:
CC Q6P499; P27449: ATP6V0C; NbExp=3; IntAct=EBI-10252783, EBI-721179;
CC Q6P499; P11912: CD79A; NbExp=3; IntAct=EBI-10252783, EBI-7797864;
CC Q6P499; Q92838: EDA; NbExp=8; IntAct=EBI-10252783, EBI-529425;
CC Q6P499; P36382: GJA5; NbExp=3; IntAct=EBI-10252783, EBI-750433;
CC Q6P499; Q8TED1: GPX8; NbExp=3; IntAct=EBI-10252783, EBI-11721746;
CC Q6P499; Q9NQG1: MANBAL; NbExp=3; IntAct=EBI-10252783, EBI-3867271;
CC Q6P499; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-10252783, EBI-11956541;
CC Q6P499; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-10252783, EBI-8638294;
CC Q6P499; Q3ZAQ7: VMA21; NbExp=3; IntAct=EBI-10252783, EBI-1055364;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6P499-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P499-2; Sequence=VSP_019445;
CC Name=3;
CC IsoId=Q6P499-3; Sequence=VSP_019446, VSP_019447;
CC -!- SIMILARITY: Belongs to the NIPA family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK312433; BAG35342.1; -; mRNA.
DR EMBL; BX640883; CAE45938.1; -; mRNA.
DR EMBL; AL031431; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471134; EAW95130.1; -; Genomic_DNA.
DR EMBL; BC001265; AAH01265.1; -; mRNA.
DR EMBL; BC063583; AAH63583.1; -; mRNA.
DR CCDS; CCDS30631.1; -. [Q6P499-1]
DR CCDS; CCDS81280.1; -. [Q6P499-3]
DR CCDS; CCDS81282.1; -. [Q6P499-2]
DR RefSeq; NP_001309783.1; NM_001322854.1. [Q6P499-1]
DR RefSeq; NP_001309784.1; NM_001322855.1. [Q6P499-1]
DR RefSeq; NP_001309785.1; NM_001322856.1. [Q6P499-1]
DR RefSeq; NP_001309786.1; NM_001322857.1. [Q6P499-1]
DR RefSeq; NP_001309791.1; NM_001322862.1. [Q6P499-2]
DR RefSeq; NP_001309792.1; NM_001322863.1. [Q6P499-2]
DR RefSeq; NP_001309794.1; NM_001322865.1. [Q6P499-3]
DR RefSeq; NP_065181.1; NM_020448.4. [Q6P499-1]
DR AlphaFoldDB; Q6P499; -.
DR BioGRID; 121434; 28.
DR IntAct; Q6P499; 11.
DR STRING; 9606.ENSP00000363520; -.
DR TCDB; 2.A.7.25.3; the drug/metabolite transporter (dmt) superfamily.
DR GlyGen; Q6P499; 1 site.
DR iPTMnet; Q6P499; -.
DR PhosphoSitePlus; Q6P499; -.
DR BioMuta; NIPAL3; -.
DR DMDM; 74737314; -.
DR EPD; Q6P499; -.
DR jPOST; Q6P499; -.
DR MassIVE; Q6P499; -.
DR MaxQB; Q6P499; -.
DR PaxDb; Q6P499; -.
DR PeptideAtlas; Q6P499; -.
DR PRIDE; Q6P499; -.
DR ProteomicsDB; 66952; -. [Q6P499-1]
DR ProteomicsDB; 66953; -. [Q6P499-2]
DR ProteomicsDB; 66954; -. [Q6P499-3]
DR Antibodypedia; 30307; 100 antibodies from 14 providers.
DR DNASU; 57185; -.
DR Ensembl; ENST00000003912.7; ENSP00000003912.3; ENSG00000001461.17. [Q6P499-2]
DR Ensembl; ENST00000358028.8; ENSP00000350722.4; ENSG00000001461.17. [Q6P499-3]
DR Ensembl; ENST00000374399.9; ENSP00000363520.4; ENSG00000001461.17. [Q6P499-1]
DR GeneID; 57185; -.
DR KEGG; hsa:57185; -.
DR MANE-Select; ENST00000374399.9; ENSP00000363520.4; NM_020448.5; NP_065181.1.
DR UCSC; uc001bjh.4; human. [Q6P499-1]
DR CTD; 57185; -.
DR DisGeNET; 57185; -.
DR GeneCards; NIPAL3; -.
DR HGNC; HGNC:25233; NIPAL3.
DR HPA; ENSG00000001461; Tissue enhanced (brain).
DR neXtProt; NX_Q6P499; -.
DR OpenTargets; ENSG00000001461; -.
DR PharmGKB; PA164723927; -.
DR VEuPathDB; HostDB:ENSG00000001461; -.
DR eggNOG; KOG2922; Eukaryota.
DR GeneTree; ENSGT00940000158233; -.
DR HOGENOM; CLU_012349_2_0_1; -.
DR InParanoid; Q6P499; -.
DR OMA; TFYLDFM; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q6P499; -.
DR TreeFam; TF313214; -.
DR PathwayCommons; Q6P499; -.
DR Reactome; R-HSA-5223345; Miscellaneous transport and binding events.
DR SignaLink; Q6P499; -.
DR BioGRID-ORCS; 57185; 8 hits in 1075 CRISPR screens.
DR ChiTaRS; NIPAL3; human.
DR GenomeRNAi; 57185; -.
DR Pharos; Q6P499; Tdark.
DR PRO; PR:Q6P499; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q6P499; protein.
DR Bgee; ENSG00000001461; Expressed in inferior vagus X ganglion and 197 other tissues.
DR ExpressionAtlas; Q6P499; baseline and differential.
DR Genevisible; Q6P499; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; IBA:GO_Central.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..406
FT /note="NIPA-like protein 3"
FT /id="PRO_0000242150"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 372
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..82
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_019445"
FT VAR_SEQ 213..226
FT /note="GSMTVVTVKAVAGM -> VLLCHPGWSAVVQS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019446"
FT VAR_SEQ 227..406
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019447"
SQ SEQUENCE 406 AA; 44742 MW; 42E87B84B01E7C88 CRC64;
MDGSHSAALK LQQLPPTSSS SAVSEASFSY KENLIGALLA IFGHLVVSIA LNLQKYCHIR
LAGSKDPRAY FKTKTWWLGL FLMLLGELGV FASYAFAPLS LIVPLSAVSV IASAIIGIIF
IKEKWKPKDF LRRYVLSFVG CGLAVVGTYL LVTFAPNSHE KMTGENVTRH LVSWPFLLYM
LVEIILFCLL LYFYKEKNAN NIVVILLLVA LLGSMTVVTV KAVAGMLVLS IQGNLQLDYP
IFYVMFVCMV ATAVYQAAFL SQASQMYDSS LIASVGYILS TTIAITAGAI FYLDFIGEDV
LHICMFALGC LIAFLGVFLI TRNRKKPIPF EPYISMDAMP GMQNMHDKGM TVQPELKASF
SYGALENNDN ISEIYAPATL PVMQEEHGSR SASGVPYRVL EHTKKE
