NPAS3_MOUSE
ID NPAS3_MOUSE Reviewed; 925 AA.
AC Q9QZQ0; Q9EQP4;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Neuronal PAS domain-containing protein 3;
DE Short=Neuronal PAS3;
DE AltName: Full=Basic-helix-loop-helix-PAS protein MOP6;
DE AltName: Full=Member of PAS protein 6;
GN Name=Npas3; Synonyms=Mop6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10534623; DOI=10.1016/s0925-4773(99)00182-3;
RA Brunskill E.W., Witte D.P., Shreiner A.B., Potter S.S.;
RT "Characterization of Npas3, a novel basic helix-loop-helix PAS gene
RT expressed in the developing mouse nervous system.";
RL Mech. Dev. 88:237-241(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 141-280.
RA Thomas R.S., Bradfield C.A.;
RT "Cloning and chromosomal localization of MOP6.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15347806; DOI=10.1073/pnas.0405310101;
RA Erbel-Sieler C., Dudley C., Zhou Y., Wu X., Estill S.J., Han T.,
RA Diaz-Arrastia R., Brunskill E.W., Potter S.S., McKnight S.L.;
RT "Behavioral and regulatory abnormalities in mice deficient in the NPAS1 and
RT NPAS3 transcription factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13648-13653(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5] {ECO:0007744|PDB:5SY7}
RP X-RAY CRYSTALLOGRAPHY (4.20 ANGSTROMS) OF 56-455 IN COMPLEXES WITH ARNT AND
RP DNA, INTERACTION WITH ARNT, HETERODIMERIZATION, AND REGION.
RX PubMed=27782878; DOI=10.7554/elife.18790;
RA Wu D., Su X., Potluri N., Kim Y., Rastinejad F.;
RT "NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family
RT as multi-ligand binding transcription factors.";
RL Elife 5:0-0(2016).
CC -!- FUNCTION: May play a broad role in neurogenesis. May control regulatory
CC pathways relevant to schizophrenia and to psychotic illness.
CC {ECO:0000269|PubMed:15347806}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Interacts with ARNT; forms a heterodimer that binds core DNA
CC sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of
CC target gene promoters (PubMed:27782878). {ECO:0000269|PubMed:27782878}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:15347806}.
CC -!- TISSUE SPECIFICITY: Detected exclusively in adult brain in inhibitory
CC interneurons.
CC -!- DEVELOPMENTAL STAGE: Expression detected between 9.5 and 11.5 dpc in
CC the developing neural tube. Was also expressed throughout the
CC neuroepithelium of the developing central nervous system between 10. 5
CC and 12.5 dpc at 14.5 dpc, the expression became restricted to the
CC neopallial layer of the cortex. At 12.5 dpc, expression was evident in
CC nonneural tissues such as the developing dermis and mesenchyme
CC surrounding the otic and nasal placodes. Expression was also detected
CC in the developing cardiac valves, limb and developing kidney.
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DR EMBL; AF173871; AAF14283.1; -; mRNA.
DR EMBL; AF168769; AAG35181.1; -; mRNA.
DR PDB; 5SY7; X-ray; 4.20 A; B=56-455.
DR PDBsum; 5SY7; -.
DR AlphaFoldDB; Q9QZQ0; -.
DR SMR; Q9QZQ0; -.
DR STRING; 10090.ENSMUSP00000098975; -.
DR iPTMnet; Q9QZQ0; -.
DR PhosphoSitePlus; Q9QZQ0; -.
DR MaxQB; Q9QZQ0; -.
DR PaxDb; Q9QZQ0; -.
DR PRIDE; Q9QZQ0; -.
DR ProteomicsDB; 295509; -.
DR MGI; MGI:1351610; Npas3.
DR eggNOG; KOG3558; Eukaryota.
DR InParanoid; Q9QZQ0; -.
DR PhylomeDB; Q9QZQ0; -.
DR ChiTaRS; Npas3; mouse.
DR PRO; PR:Q9QZQ0; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9QZQ0; protein.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007626; P:locomotory behavior; IMP:MGI.
DR GO; GO:0042711; P:maternal behavior; IGI:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0001964; P:startle response; IGI:MGI.
DR CDD; cd00130; PAS; 2.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR InterPro; IPR013655; PAS_fold_3.
DR Pfam; PF00989; PAS; 1.
DR Pfam; PF08447; PAS_3; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..925
FT /note="Neuronal PAS domain-containing protein 3"
FT /id="PRO_0000127409"
FT DOMAIN 58..111
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 152..222
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 324..394
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 398..441
FT /note="PAC"
FT REGION 60..71
FT /note="DNA-binding"
FT /evidence="ECO:0000269|PubMed:27782878"
FT REGION 119..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 576..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 664..774
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 461..511
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..548
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 601..617
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 664..699
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 141
FT /note="R -> L (in Ref. 2; AAG35181)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="G -> E (in Ref. 2; AAG35181)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="F -> L (in Ref. 2; AAG35181)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 925 AA; 100458 MW; B6B07C9B6755B938 CRC64;
MGRAGAAANG TPQNVQGITS YQQRITAQHP LPNQSECRKI YRYDGIYCES TYQNLQALRK
EKSRDAARSR RGKENFEFYE LAKLLPLPAA ITSQLDKASI IRLTISYLKM RDFANQGDPP
WNLRMEGPPP NTSVKGAQRR RSPSALAIEV FEAHLGSHIL QSLDGFVFAL NQEGKFLYIS
ETVSIYLGLS QVELTGSSVF DYVHPGDHVE MAEQLGMKLP PGRGLLSQGT TEDAASSASS
SSQSETPEPV ETTSPSLLTT DNTLERSFFI RMKSTLTKRG VHIKSSGYKV IHITGRLRLR
VPLSHGRTVP SQIMGLVVVA HALPPPTINE VRIDCHMFVT RVNMDLNIIY CENRISDYMD
LTPVDIVGKR CYHFIHAEDV EGIRHSHLDL LNKGQCVTKY YRWMQKNGGY IWIQSSATIA
INAKNANEKN IIWVNYLLSN PEYKDTPMDI AQLPHLPEKA SESSETSDSE SDSKDTSGIT
EDNENSKSDE KGNQSENSED PEPDRKKSGS ACDNDMNCND DGHSSSNPDS RDSDDSFEHS
DFEHPKAAED GFGALGPMQI KVERYVESEA DLRLQPCESL TSDSAKDSDS ANEAGAQASS
KHQKRKRRRK RQKGGSASRR RLSSASSPGL DAGLVEPPRL LSSPHSASVL KIKTEIAEPI
NFDNESSIWN YPPNREISRN ESPYSMTKPP TSEHFPSPQG QGGSIGGGGA LHVAIPDSVL
TPPGADGTAG RKTQFSGTAP VPSDPLSPPL SASPRDKHPG GGAGSGGGGP GASNSLLYTG
DLEALQRLQA GNVVLPLVHR VTGTLAATST AAQRVYTTGT IRYAPAEVTL AMQGNLLPNA
HAVNFVDVNS PGFGLDPKTP MEMLYHHVHR LNMSGPFGGA VSAASLTQMP GGNVFTTAEG
LFSTLPFPVY SNGIHAAQTL ERKED
