NPASH_CAEEL
ID NPASH_CAEEL Reviewed; 676 AA.
AC G5EFL9;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=PAS domain-containing protein cky-1 {ECO:0000305};
DE AltName: Full=CKY homolog {ECO:0000312|WormBase:C15C8.2};
DE AltName: Full=bHLH-PAS transcription factor cky-1 {ECO:0000303|PubMed:17628356};
GN Name=cky-1 {ECO:0000312|WormBase:C15C8.2};
GN Synonyms=cNXFL {ECO:0000312|EMBL:BAE53417.1};
GN ORFNames=C15C8.2 {ECO:0000312|WormBase:C15C8.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:BAE53417.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH AHA-1, AND
RP SUBCELLULAR LOCATION.
RX PubMed=17628356; DOI=10.1016/j.gene.2007.06.005;
RA Ooe N., Saito K., Oeda K., Nakatuka I., Kaneko H.;
RT "Characterization of Drosophila and Caenorhabditis elegans NXF-like-
RT factors, putative homologs of mammalian NXF.";
RL Gene 400:122-130(2007).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Transcription factor (PubMed:17628356). Efficient DNA binding
CC requires dimerization with another bHLH protein, such as ARNT homolog
CC aha-1 (PubMed:17628356). Regulates transcription of target genes,
CC probably acting in complex with aha-1 (PubMed:17628356).
CC {ECO:0000269|PubMed:17628356}.
CC -!- SUBUNIT: Heterodimer; efficient DNA binding requires dimerization with
CC another bHLH protein (PubMed:17628356). Forms a heterodimer with ARNT
CC homolog aha-1; binds DNA as heterodimer (PubMed:17628356).
CC {ECO:0000269|PubMed:17628356}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000303|PubMed:17628356}.
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DR EMBL; AB096001; BAE53417.1; -; mRNA.
DR EMBL; BX284605; CAP46770.1; -; Genomic_DNA.
DR PIR; T19307; T19307.
DR RefSeq; NP_001122849.1; NM_001129377.2.
DR RefSeq; NP_506184.2; NM_073783.4.
DR SMR; G5EFL9; -.
DR IntAct; G5EFL9; 3.
DR STRING; 6239.C15C8.2b; -.
DR EnsemblMetazoa; C15C8.2.1; C15C8.2.1; WBGene00000521.
DR GeneID; 179743; -.
DR KEGG; cel:CELE_C15C8.2; -.
DR CTD; 179743; -.
DR WormBase; C15C8.2; CE41867; WBGene00000521; cky-1.
DR eggNOG; ENOG502QRXX; Eukaryota.
DR GeneTree; ENSGT00530000064165; -.
DR HOGENOM; CLU_430370_0_0_1; -.
DR OMA; MLMVTRS; -.
DR OrthoDB; 572843at2759; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00000521; Expressed in pharyngeal muscle cell (C elegans) and 2 other tissues.
DR GO; GO:0005634; C:nucleus; IC:WormBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00130; PAS; 2.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR SUPFAM; SSF55785; SSF55785; 2.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50112; PAS; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..676
FT /note="PAS domain-containing protein cky-1"
FT /id="PRO_0000456247"
FT DOMAIN 76..129
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 165..215
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT REGION 76..89
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 90..129
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 676 AA; 75796 MW; C5A399EC78BA315C CRC64;
MSTTVPLHAS SFSSGTTGAG GGVQQALQLQ HCAYHVGASV GSSGSALNNN SINVPNNNTM
GMSSAGSSNG SNLVNGQQRS TRGASKQRRD QINVEIQKLR DLLPLSDLIK DRLFQLQVMS
LGCIFIRKHR YQQTVLQPQL QMLQMQMSSP MPRGIDICKA LRGFMLMVTR SGKILHVSDN
ASEYLGHSVE EIMCQGDSIY DLVDGRDHGA VQAELASGPP GAATFPEERV FICRLNLART
AKRQLQYHKF VLFQGRYIQP AEFYQQLNAQ NSQPDCDQPV FSAYCQPLIN PENAEGMSTG
NTHVFSTQHY LDMKFKEADT MASQHLGFSK EQLKGMSWYG MIHPNHVPEI AHKHRLLCQE
KEGSVLALIR LQAANGEWIW LHTVFSIRPN NELNSDGKRL RHVIHCFHQK LTDLEAATLQ
ANSWIYSMRH TYPTVFSCQD SPPPSEEQQP SSPQTPPFTE QPPMLVEQKP LIGAGIAPGF
PSLDYNQIKV EIPMRPINTQ IPLFTPESSS PESSASLHTS LLPHHNAFPL DILEDILPSN
DVLPELKDEV DEILRQVEQS PATSPAHSFP SNVNFGHRHS MPNAFDSAEL YKYLGPALFD
GNFAMQHQHH LQQQHQSQPN HPLQMTPIPP MSCPPSNYGQ SNFLFHHQHL NQHLHHSQPP
HPYHFDYYNR KRSWAV
