NPBW1_HUMAN
ID NPBW1_HUMAN Reviewed; 328 AA.
AC P48145; Q6NTC7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Neuropeptides B/W receptor type 1;
DE AltName: Full=G-protein coupled receptor 7;
GN Name=NPBWR1; Synonyms=GPR7;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7590751; DOI=10.1006/geno.1995.1109;
RA O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S.,
RA Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L.,
RA George S.R.;
RT "The cloning and chromosomal mapping of two novel human opioid-
RT somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete
RT areas of the brain.";
RL Genomics 28:84-91(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY, AND INTERACTION WITH NEUROPEPTIDES B AND W.
RX PubMed=12401809; DOI=10.1074/jbc.m206396200;
RA Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V.,
RA Lucchetti J., Detheux M., Parmentier M.;
RT "Identification of natural ligands for the orphan G protein-coupled
RT receptors GPR7 and GPR8.";
RL J. Biol. Chem. 278:776-783(2003).
RN [5]
RP INTERACTION WITH NEUROPEPTIDES B AND W.
RX PubMed=12719537; DOI=10.1073/pnas.0837789100;
RA Tanaka H., Yoshida T., Miyamoto N., Motoike T., Kurosu H., Shibata K.,
RA Yamanaka A., Williams S.C., Richardson J.A., Tsujino N., Garry M.G.,
RA Lerner M.R., King D.S., O'Dowd B.F., Sakurai T., Yanagisawa M.;
RT "Characterization of a family of endogenous neuropeptide ligands for the G
RT protein-coupled receptors GPR7 and GPR8.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:6251-6256(2003).
RN [6]
RP VARIANT [LARGE SCALE ANALYSIS] GLN-19.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Interacts specifically with a number of opioid ligands.
CC Receptor for neuropeptides B and W, which may be involved in
CC neuroendocrine system regulation, food intake and the organization of
CC other signals. Has a higher affinity for neuropeptide B.
CC -!- INTERACTION:
CC P48145; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-13061492, EBI-947187;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Found in cerebellum and frontal cortex. Detected at
CC high levels in hippocampus, amygdala and trachea; at moderate levels in
CC fetal brain, pituitary gland and prostate. Not in caudate, accumbens,
CC kidney or liver. Also detected at high levels in lung carcinoma.
CC {ECO:0000269|PubMed:12401809}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U22491; AAC50197.1; -; Genomic_DNA.
DR EMBL; CH471068; EAW86722.1; -; Genomic_DNA.
DR EMBL; BC069117; AAH69117.1; -; mRNA.
DR EMBL; BC107101; AAI07102.1; -; mRNA.
DR CCDS; CCDS6151.1; -.
DR PIR; I38973; I38973.
DR RefSeq; NP_005276.2; NM_005285.3.
DR AlphaFoldDB; P48145; -.
DR SMR; P48145; -.
DR BioGRID; 109092; 3.
DR IntAct; P48145; 1.
DR STRING; 9606.ENSP00000330284; -.
DR BindingDB; P48145; -.
DR ChEMBL; CHEMBL1293293; -.
DR GuidetoPHARMACOLOGY; 303; -.
DR GlyGen; P48145; 4 sites, 1 O-linked glycan (1 site).
DR PhosphoSitePlus; P48145; -.
DR BioMuta; NPBWR1; -.
DR DMDM; 215274092; -.
DR MassIVE; P48145; -.
DR PaxDb; P48145; -.
DR PeptideAtlas; P48145; -.
DR PRIDE; P48145; -.
DR Antibodypedia; 82634; 218 antibodies from 29 providers.
DR DNASU; 2831; -.
DR Ensembl; ENST00000674939.1; ENSP00000501711.1; ENSG00000288611.1.
DR GeneID; 2831; -.
DR KEGG; hsa:2831; -.
DR MANE-Select; ENST00000674939.1; ENSP00000501711.1; NM_005285.5; NP_005276.2.
DR UCSC; uc011ldu.3; human.
DR CTD; 2831; -.
DR DisGeNET; 2831; -.
DR GeneCards; NPBWR1; -.
DR HGNC; HGNC:4522; NPBWR1.
DR HPA; ENSG00000288611; Tissue enhanced (brain, liver).
DR MIM; 600730; gene.
DR neXtProt; NX_P48145; -.
DR PharmGKB; PA28912; -.
DR VEuPathDB; HostDB:ENSG00000288611; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000161936; -.
DR HOGENOM; CLU_009579_8_1_1; -.
DR InParanoid; P48145; -.
DR OMA; PESVWWK; -.
DR OrthoDB; 1011272at2759; -.
DR PhylomeDB; P48145; -.
DR TreeFam; TF315737; -.
DR PathwayCommons; P48145; -.
DR Reactome; R-HSA-375276; Peptide ligand-binding receptors.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; P48145; -.
DR SIGNOR; P48145; -.
DR BioGRID-ORCS; 2831; 14 hits in 1056 CRISPR screens.
DR GeneWiki; Neuropeptides_B/W_receptor_1; -.
DR GenomeRNAi; 2831; -.
DR Pharos; P48145; Tchem.
DR PRO; PR:P48145; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; P48145; protein.
DR Bgee; ENSG00000288611; Expressed in gall bladder and 48 other tissues.
DR ExpressionAtlas; P48145; differential.
DR Genevisible; P48145; HS.
DR GO; GO:0016021; C:integral component of membrane; IC:HGNC-UCL.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0004985; F:G protein-coupled opioid receptor activity; TAS:ProtInc.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0042923; F:neuropeptide binding; IBA:GO_Central.
DR GO; GO:0008188; F:neuropeptide receptor activity; IEA:InterPro.
DR GO; GO:0042277; F:peptide binding; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:HGNC-UCL.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0019222; P:regulation of metabolic process; IEA:Ensembl.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR009150; Neuropept_B/W_rcpt.
DR InterPro; IPR027348; NPBW1.
DR PANTHER; PTHR24229:SF47; PTHR24229:SF47; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01855; NRPEPTIDEWR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..328
FT /note="Neuropeptides B/W receptor type 1"
FT /id="PRO_0000069518"
FT TOPO_DOM 1..37
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..97
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..112
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..132
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..177
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..202
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..273
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..298
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..328
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 109..188
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 19
FT /note="P -> Q (in a breast cancer sample; somatic mutation;
FT dbSNP:rs772418985)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035765"
FT VARIANT 135
FT /note="Y -> F (in dbSNP:rs33977775)"
FT /id="VAR_047788"
FT VARIANT 319
FT /note="R -> C (in dbSNP:rs36068168)"
FT /id="VAR_047789"
FT CONFLICT 296
FT /note="S -> T (in Ref. 1; AAC50197)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 36103 MW; 7A90F85AD64A3980 CRC64;
MDNASFSEPW PANASGPDPA LSCSNASTLA PLPAPLAVAV PVVYAVICAV GLAGNSAVLY
VLLRAPRMKT VTNLFILNLA IADELFTLVL PINIADFLLR QWPFGELMCK LIVAIDQYNT
FSSLYFLTVM SADRYLVVLA TAESRRVAGR TYSAARAVSL AVWGIVTLVV LPFAVFARLD
DEQGRRQCVL VFPQPEAFWW RASRLYTLVL GFAIPVSTIC VLYTTLLCRL HAMRLDSHAK
ALERAKKRVT FLVVAILAVC LLCWTPYHLS TVVALTTDLP QTPLVIAISY FITSLSYANS
CLNPFLYAFL DASFRRNLRQ LITCRAAA
