NPC1_ARATH
ID NPC1_ARATH Reviewed; 533 AA.
AC Q8L7Y9; Q9LML5;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Non-specific phospholipase C1;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=NPC1; OrderedLocusNames=At1g07230; ORFNames=F10K1.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND LACK OF INDUCTION BY PHOSPHATE DEPRIVATION.
RX PubMed=15618226; DOI=10.1074/jbc.m408799200;
RA Nakamura Y., Awai K., Masuda T., Yoshioka Y., Takamiya K., Ohta H.;
RT "A novel phosphatidylcholine-hydrolyzing phospholipase C induced by
RT phosphate starvation in Arabidopsis.";
RL J. Biol. Chem. 280:7469-7476(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=20507939; DOI=10.1093/mp/ssq005;
RA Wimalasekera R., Pejchar P., Holk A., Martinec J., Scherer G.F.;
RT "Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with
RT roles in root development and brassinolide signaling in Arabidopsis
RT thaliana.";
RL Mol. Plant 3:610-625(2010).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. {ECO:0000269|PubMed:20507939}.
CC -!- INDUCTION: Not induced by inorganic phosphate deprivation.
CC -!- SIMILARITY: Belongs to the bacterial phospholipase C family.
CC {ECO:0000305}.
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DR EMBL; AB084291; BAC22506.1; -; mRNA.
DR EMBL; AC067971; AAF82197.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28095.1; -; Genomic_DNA.
DR EMBL; AY124005; AAM74513.1; -; mRNA.
DR EMBL; BT020583; AAW80856.1; -; mRNA.
DR PIR; E86207; E86207.
DR RefSeq; NP_172203.2; NM_100597.4.
DR AlphaFoldDB; Q8L7Y9; -.
DR SMR; Q8L7Y9; -.
DR STRING; 3702.AT1G07230.1; -.
DR PaxDb; Q8L7Y9; -.
DR PRIDE; Q8L7Y9; -.
DR ProteomicsDB; 250518; -.
DR EnsemblPlants; AT1G07230.1; AT1G07230.1; AT1G07230.
DR GeneID; 837234; -.
DR Gramene; AT1G07230.1; AT1G07230.1; AT1G07230.
DR KEGG; ath:AT1G07230; -.
DR Araport; AT1G07230; -.
DR TAIR; locus:2007357; AT1G07230.
DR eggNOG; ENOG502QPJ0; Eukaryota.
DR HOGENOM; CLU_029943_1_0_1; -.
DR InParanoid; Q8L7Y9; -.
DR OMA; ECCSYMS; -.
DR OrthoDB; 480237at2759; -.
DR PhylomeDB; Q8L7Y9; -.
DR BioCyc; ARA:AT1G07230-MON; -.
DR BRENDA; 3.1.4.3; 399.
DR PRO; PR:Q8L7Y9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L7Y9; baseline and differential.
DR Genevisible; Q8L7Y9; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0000137; C:Golgi cis cisterna; HDA:TAIR.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0009395; P:phospholipid catabolic process; IBA:GO_Central.
DR Gene3D; 3.40.720.10; -; 2.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR007312; Phosphoesterase.
DR PANTHER; PTHR31956; PTHR31956; 1.
DR Pfam; PF04185; Phosphoesterase; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..533
FT /note="Non-specific phospholipase C1"
FT /id="PRO_0000424783"
FT CONFLICT 360
FT /note="P -> S (in Ref. 2; AAF82197)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 533 AA; 60048 MW; 21846EB025425E24 CRC64;
MAFRRVLTTV ILFCYLLISS QSIEFKNSQK PHKIQGPIKT IVVVVMENRS FDHILGWLKS
TRPEIDGLTG KESNPLNVSD PNSKKIFVSD DAVFVDMDPG HSFQAIREQI FGSNDTSGDP
KMNGFAQQSE SMEPGMAKNV MSGFKPEVLP VYTELANEFG VFDRWFASVP TSTQPNRFYV
HSATSHGCSS NVKKDLVKGF PQKTIFDSLD ENGLSFGIYY QNIPATFFFK SLRRLKHLVK
FHSYALKFKL DAKLGKLPNY SVVEQRYFDI DLFPANDDHP SHDVAAGQRF VKEVYETLRS
SPQWKEMALL ITYDEHGGFY DHVPTPVKGV PNPDGIIGPD PFYFGFDRLG VRVPTFLISP
WIEKGTVIHE PEGPTPHSQF EHSSIPATVK KLFNLKSHFL TKRDAWAGTF EKYFRIRDSP
RQDCPEKLPE VKLSLRPWGA KEDSKLSEFQ VELIQLASQL VGDHLLNSYP DIGKNMTVSE
GNKYAEDAVQ KFLEAGMAAL EAGADENTIV TMRPSLTTRT SPSEGTNKYI GSY
