ID   NPC2_ARATH              Reviewed;         514 AA.
AC   O81020;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Non-specific phospholipase C2;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   Name=NPC2; OrderedLocusNames=At2g26870; ORFNames=F12C20.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND LACK OF INDUCTION BY PHOSPHATE DEPRIVATION.
RX   PubMed=15618226; DOI=10.1074/jbc.m408799200;
RA   Nakamura Y., Awai K., Masuda T., Yoshioka Y., Takamiya K., Ohta H.;
RT   "A novel phosphatidylcholine-hydrolyzing phospholipase C induced by
RT   phosphate starvation in Arabidopsis.";
RL   J. Biol. Chem. 280:7469-7476(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=20507939; DOI=10.1093/mp/ssq005;
RA   Wimalasekera R., Pejchar P., Holk A., Martinec J., Scherer G.F.;
RT   "Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with
RT   roles in root development and brassinolide signaling in Arabidopsis
RT   thaliana.";
RL   Mol. Plant 3:610-625(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves, stems, flowers and siliques.
CC       {ECO:0000269|PubMed:20507939}.
CC   -!- INDUCTION: Not induced by inorganic phosphate deprivation.
CC   -!- SIMILARITY: Belongs to the bacterial phospholipase C family.
CC       {ECO:0000305}.
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DR   EMBL; AB084292; BAC22507.1; -; mRNA.
DR   EMBL; AC005168; AAC32238.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07902.1; -; Genomic_DNA.
DR   PIR; T02648; T02648.
DR   RefSeq; NP_180255.1; NM_128244.4.
DR   AlphaFoldDB; O81020; -.
DR   SMR; O81020; -.
DR   STRING; 3702.AT2G26870.1; -.
DR   PaxDb; O81020; -.
DR   PRIDE; O81020; -.
DR   ProteomicsDB; 251165; -.
DR   EnsemblPlants; AT2G26870.1; AT2G26870.1; AT2G26870.
DR   GeneID; 817228; -.
DR   Gramene; AT2G26870.1; AT2G26870.1; AT2G26870.
DR   KEGG; ath:AT2G26870; -.
DR   Araport; AT2G26870; -.
DR   TAIR; locus:2039563; AT2G26870.
DR   eggNOG; ENOG502QPJ0; Eukaryota.
DR   HOGENOM; CLU_029943_1_0_1; -.
DR   InParanoid; O81020; -.
DR   OMA; EPFTPRV; -.
DR   OrthoDB; 430314at2759; -.
DR   PhylomeDB; O81020; -.
DR   BRENDA; 3.1.4.3; 399.
DR   PRO; PR:O81020; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O81020; baseline and differential.
DR   Genevisible; O81020; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR   GO; GO:0004629; F:phospholipase C activity; IDA:TAIR.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR   GO; GO:0048229; P:gametophyte development; IGI:TAIR.
DR   GO; GO:0009395; P:phospholipid catabolic process; IDA:TAIR.
DR   GO; GO:0048364; P:root development; IGI:TAIR.
DR   Gene3D; 3.40.720.10; -; 2.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR007312; Phosphoesterase.
DR   PANTHER; PTHR31956; PTHR31956; 1.
DR   Pfam; PF04185; Phosphoesterase; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..514
FT                   /note="Non-specific phospholipase C2"
FT                   /id="PRO_0000424784"
SQ   SEQUENCE   514 AA;  57681 MW;  0575269A64F1C2CE CRC64;
     MSIKAFALIQ LLSVTILYNH VHATSPIKTI VVVVMENRSF DHMLGWMKKL NPEINGVDGS
     ESNPVSVSDP SSRKIKFGSG SHYVDPDPGH SFQAIREQVF GSNDTSMDPP PMNGFVQQAY
     SEDPSGNMSA SVMNGFEPDK VPVYKSLVSE FAVFDRWFAS VPSSTQPNRM FVHSGTSAGA
     TSNNPISLAK GYPQRTIFDN LDDEEFSFGI YYQNIPAVLF YQSLRKLKYV FKFHSYGNSF
     KDHAKQGKLP AYTVIEQRYM DTLLEPASDD HPSHDVYQGQ KFIKEVYETL RASPQWNETL
     LIITYDEHGG YFDHVPTPVR NVPSPDGIVG PDPFLFQFNR LGIRVPTIAV SPWIEKGTVV
     HGPNGSPFPS SEYEHSSIPA TVKKLFNLSS PFLTKRDEWA GTFENILQIR KEPRTDCPET
     LPEPVKIRMG EANEKALLTE FQQELVQLAA VLKGDNMLTT FPKEISKGMT VIEGKRYMED
     AMKRFLEAGR MALSMGANKE ELVHMKTSLT GRRP