NPC2_YEAST
ID NPC2_YEAST Reviewed; 173 AA.
AC Q12408; D6VRU9;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Phosphatidylglycerol/phosphatidylinositol transfer protein;
DE Short=PG/PI-TP;
DE AltName: Full=NPC2 homolog;
DE Flags: Precursor;
GN Name=NPC2; OrderedLocusNames=YDL046W; ORFNames=D2699;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9046088;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<65::aid-yea50>3.0.co;2-t;
RA Saren A.-M., Laamanen P., Lejarcegui J.B., Paulin L.;
RT "The sequence of a 36.7 kb segment on the left arm of chromosome IV from
RT Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames
RT (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1
RT and 11 new ORFs.";
RL Yeast 13:65-71(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP PROTEIN SEQUENCE OF 59-69 AND 135-173, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RA Bienvenut W.V., Peters C.;
RL Submitted (MAY-2005) to UniProtKB.
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Catalyzes the intermembrane transfer of phosphatidylglycerol
CC and phosphatidylinositol. {ECO:0000250}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 2836 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NPC2 family. {ECO:0000305}.
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DR EMBL; Z71781; CAA96443.1; -; Genomic_DNA.
DR EMBL; Z74094; CAA98606.1; -; Genomic_DNA.
DR EMBL; AY692671; AAT92690.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11809.1; -; Genomic_DNA.
DR PIR; S67579; S67579.
DR RefSeq; NP_010237.1; NM_001180105.1.
DR PDB; 6R4M; X-ray; 2.80 A; A/B/C=1-173.
DR PDB; 6R4N; X-ray; 2.90 A; A/B/C/D/E/F/G/H/I=1-173.
DR PDBsum; 6R4M; -.
DR PDBsum; 6R4N; -.
DR AlphaFoldDB; Q12408; -.
DR SMR; Q12408; -.
DR BioGRID; 32012; 27.
DR DIP; DIP-5137N; -.
DR IntAct; Q12408; 2.
DR STRING; 4932.YDL046W; -.
DR TCDB; 2.A.6.6.3; the resistance-nodulation-cell division (rnd) superfamily.
DR MaxQB; Q12408; -.
DR PaxDb; Q12408; -.
DR PRIDE; Q12408; -.
DR EnsemblFungi; YDL046W_mRNA; YDL046W; YDL046W.
DR GeneID; 851514; -.
DR KEGG; sce:YDL046W; -.
DR SGD; S000002204; NPC2.
DR VEuPathDB; FungiDB:YDL046W; -.
DR eggNOG; KOG4680; Eukaryota.
DR HOGENOM; CLU_097982_0_1_1; -.
DR InParanoid; Q12408; -.
DR OMA; QTYDLCE; -.
DR BioCyc; YEAST:G3O-29465-MON; -.
DR PRO; PR:Q12408; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12408; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0000328; C:fungal-type vacuole lumen; IDA:SGD.
DR GO; GO:0032934; F:sterol binding; IDA:SGD.
DR GO; GO:0032366; P:intracellular sterol transport; IGI:SGD.
DR GO; GO:0015918; P:sterol transport; IDA:SGD.
DR CDD; cd00917; PG-PI_TP; 1.
DR Gene3D; 2.70.220.10; -; 1.
DR InterPro; IPR036846; GM2-AP_sf.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR003172; ML_dom.
DR InterPro; IPR033917; ML_PG-PI_TP.
DR InterPro; IPR039670; NPC2-like.
DR PANTHER; PTHR11306; PTHR11306; 1.
DR Pfam; PF02221; E1_DerP2_DerF2; 1.
DR SMART; SM00737; ML; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Lipid transport;
KW Reference proteome; Signal; Transport; Vacuole.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..34
FT /evidence="ECO:0000250"
FT /id="PRO_0000019897"
FT CHAIN 35..173
FT /note="Phosphatidylglycerol/phosphatidylinositol transfer
FT protein"
FT /id="PRO_0000019898"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 53..66
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 71..81
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 90..97
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 100..108
FT /evidence="ECO:0007829|PDB:6R4M"
FT HELIX 109..115
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 127..137
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 140..142
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 144..154
FT /evidence="ECO:0007829|PDB:6R4M"
FT STRAND 160..170
FT /evidence="ECO:0007829|PDB:6R4M"
SQ SEQUENCE 173 AA; 19063 MW; 3779888CD885A3E9 CRC64;
MTHSLKALFA LLFLYTAAVN AGVIGIFNAL PPPNTKPING ESPLYQCDIL DKQLVEIKEV
NLDPNPPVRG ENLTISANGE VFETIEEGAY IDVEVRLGYI RLLSQTFDLC ETLEDNDIEG
LSCPIEPGEY NIKKIVEIPG EVPPGKYVVV ARAYTEKDDL ITCLTGEVIF PPR
