ID   NPC6_ARATH              Reviewed;         520 AA.
AC   Q8H965; Q9SMN8;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Non-specific phospholipase C6;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   Name=NPC6; OrderedLocusNames=At3g48610;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND LACK OF INDUCTION BY PHOSPHATE DEPRIVATION.
RX   PubMed=15618226; DOI=10.1074/jbc.m408799200;
RA   Nakamura Y., Awai K., Masuda T., Yoshioka Y., Takamiya K., Ohta H.;
RT   "A novel phosphatidylcholine-hydrolyzing phospholipase C induced by
RT   phosphate starvation in Arabidopsis.";
RL   J. Biol. Chem. 280:7469-7476(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=20507939; DOI=10.1093/mp/ssq005;
RA   Wimalasekera R., Pejchar P., Holk A., Martinec J., Scherer G.F.;
RT   "Plant phosphatidylcholine-hydrolyzing phospholipases C NPC3 and NPC4 with
RT   roles in root development and brassinolide signaling in Arabidopsis
RT   thaliana.";
RL   Mol. Plant 3:610-625(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC       siliques. {ECO:0000269|PubMed:20507939}.
CC   -!- INDUCTION: Not induced by inorganic phosphate deprivation.
CC   -!- SIMILARITY: Belongs to the bacterial phospholipase C family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB62350.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB084296; BAC22511.1; -; mRNA.
DR   EMBL; AL133315; CAB62350.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002686; AEE78437.1; -; Genomic_DNA.
DR   PIR; T46205; T46205.
DR   RefSeq; NP_190430.2; NM_114720.4.
DR   AlphaFoldDB; Q8H965; -.
DR   SMR; Q8H965; -.
DR   STRING; 3702.AT3G48610.1; -.
DR   PaxDb; Q8H965; -.
DR   PRIDE; Q8H965; -.
DR   ProteomicsDB; 250541; -.
DR   EnsemblPlants; AT3G48610.1; AT3G48610.1; AT3G48610.
DR   GeneID; 824021; -.
DR   Gramene; AT3G48610.1; AT3G48610.1; AT3G48610.
DR   KEGG; ath:AT3G48610; -.
DR   Araport; AT3G48610; -.
DR   TAIR; locus:2114470; AT3G48610.
DR   eggNOG; ENOG502QPJ0; Eukaryota.
DR   HOGENOM; CLU_029943_1_0_1; -.
DR   InParanoid; Q8H965; -.
DR   OMA; FYQDAYL; -.
DR   OrthoDB; 430314at2759; -.
DR   PhylomeDB; Q8H965; -.
DR   BRENDA; 3.1.4.3; 399.
DR   PRO; PR:Q8H965; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q8H965; baseline and differential.
DR   Genevisible; Q8H965; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004629; F:phospholipase C activity; IDA:TAIR.
DR   GO; GO:0048229; P:gametophyte development; IGI:TAIR.
DR   GO; GO:0009395; P:phospholipid catabolic process; IDA:TAIR.
DR   GO; GO:0048364; P:root development; IGI:TAIR.
DR   Gene3D; 3.40.720.10; -; 2.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR007312; Phosphoesterase.
DR   PANTHER; PTHR31956; PTHR31956; 1.
DR   Pfam; PF04185; Phosphoesterase; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..520
FT                   /note="Non-specific phospholipase C6"
FT                   /id="PRO_0000424788"
SQ   SEQUENCE   520 AA;  57911 MW;  FB01292F133152D9 CRC64;
     MKPSSASRFS LTFSHFLTLY CLLTQTHVAQ GSHQWQSPIK TVVVLVLENR SFDHLLGWMK
     NSVNPTINGV TGQECNPVPN STQTICFTSD AEFVDPDPGH SFEAVEQQVF GSGPGQIPSM
     MGFVEQALSM PGNLSETVMK GFRPEAVPVY AELVKEFAVF DRWFSSIPGP TQPNRLFVYS
     ATSHGSTSHV KKQLAQGYPQ KTIFDSLHSN DIDFGIYFQN IPTTLFYRNL RQLKYIFNLH
     QYDLKFKKDA AKGKLPSLTV IEPRYFDLKG LPANDDHPSH DVANGQKLVK EVYEALRSSP
     QWNETLLVIT YDEHGGFYDH VKTPYVGIPN PDGNTGPAPG FFKFDRLGVR VPTIMVSPWI
     QKGTVVSEAK GPTESSEYEH SSIPATIKKL FNLSSNFLTH RDAWAATFED VVSHLTTPRT
     DCPMTLPEVA PMRATEPKED AALSEFQGEV VQLAAVLNGD HFLSSFPEEI GKKMTVKQAH
     EYVKGATSRF IRASKEAMKL GADKSAIVDM RSSLTTRPHN