ID   NPF2_LEPDE              Reviewed;           8 AA.
AC   P85514;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   02-JUN-2021, entry version 18.
DE   RecName: Full=Neuropeptide NPF-2;
DE            Short=Led-NPF-2;
DE   AltName: Full=Short neuropeptide F II;
OS   Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora
OS   decemlineata).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC   Chrysomeloidea; Chrysomelidae; Chrysomelinae; Doryphorini; Leptinotarsa.
OX   NCBI_TaxID=7539;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND AMIDATION AT PHE-8.
RC   TISSUE=Cerebral ganglion {ECO:0000269|PubMed:8814784};
RX   PubMed=8814784; DOI=10.1016/0965-1748(95)00104-2;
RA   Spittaels K., Verhaert P., Shaw C., Johnston R.N., Devreese B.,
RA   Van Beeumen J., De Loof A.;
RT   "Insect neuropeptide F (NPF)-related peptides: isolation from Colorado
RT   potato beetle (Leptinotarsa decemlineata) brain.";
RL   Insect Biochem. Mol. Biol. 26:375-382(1996).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=15081426; DOI=10.1016/j.bbrc.2004.03.136;
RA   Huybrechts J., De Loof A., Schoofs L.;
RT   "Diapausing Colorado potato beetles are devoid of short neuropeptide F I
RT   and II.";
RL   Biochem. Biophys. Res. Commun. 317:909-916(2004).
CC   -!- FUNCTION: Neuropeptide. May be involved in the regulation of adult
CC       diapause. {ECO:0000269|PubMed:15081426}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9VIQ0}.
CC   -!- DEVELOPMENTAL STAGE: Found in the brain of non-diapausing beetles, but
CC       not in diapausing beetles. {ECO:0000269|PubMed:15081426}.
CC   -!- MASS SPECTROMETRY: Mass=958.4; Method=MALDI; Note=Using a Fisons mass
CC       spectrometer.; Evidence={ECO:0000269|PubMed:8814784};
CC   -!- MASS SPECTROMETRY: Mass=958.1; Method=MALDI; Note=Using a Bruker mass
CC       spectrometer.; Evidence={ECO:0000269|PubMed:8814784};
CC   -!- SIMILARITY: Belongs to the NPY family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Hormone; Neuropeptide; Secreted.
FT   PEPTIDE         1..8
FT                   /note="Neuropeptide NPF-2"
FT                   /evidence="ECO:0000269|PubMed:8814784"
FT                   /id="PRO_0000328938"
FT   MOD_RES         8
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:8814784"
SQ   SEQUENCE   8 AA;  959 MW;  9CD407340735A76D CRC64;
     APSLRLRF