NPFF1_RAT
ID NPFF1_RAT Reviewed; 432 AA.
AC Q9EP86;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Neuropeptide FF receptor 1;
DE AltName: Full=G-protein coupled receptor 147;
DE AltName: Full=RFamide-related peptide receptor OT7T022;
GN Name=Npffr1; Synonyms=Gpr147, Npff1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Brain stem;
RX PubMed=11025660; DOI=10.1038/35036326;
RA Hinuma S., Shintani Y., Fukusumi S., Iijima N., Matsumoto Y., Hosoya M.,
RA Fujii R., Watanabe T., Kikuchi K., Terao Y., Yano T., Yamamoto T.,
RA Kawamata Y., Habata Y., Asada M., Kitada C., Kurokawa T., Onda H.,
RA Nishimura O., Tanaka M., Ibata Y., Fujino M.;
RT "New neuropeptides containing carboxy-terminal RFamide and their receptor
RT in mammals.";
RL Nat. Cell Biol. 2:703-708(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Hypothalamus;
RX PubMed=11024015; DOI=10.1074/jbc.m004385200;
RA Bonini J.A., Jones K.A., Adham N., Forray C., Artymyshyn R., Durkin M.M.,
RA Smith K.E., Tamm J.A., Boteju L.W., Lakhlani P.P., Raddatz R., Yao W.-J.,
RA Ogozalek K.L., Boyle N., Kouranova E.V., Quan Y., Vaysse P.J., Wetzel J.M.,
RA Branchek T.A., Gerald C., Borowsky B.;
RT "Identification and characterization of two G protein-coupled receptors for
RT neuropeptide FF.";
RL J. Biol. Chem. 275:39324-39331(2000).
RN [3]
RP PROTEIN SEQUENCE OF 185-194, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (JUL-2007) to UniProtKB.
CC -!- FUNCTION: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide)
CC neuropeptides, also known as morphine-modulating peptides. Can also be
CC activated by a variety of naturally occurring or synthetic FMRF-amide
CC like ligands. This receptor mediates its action by association with G
CC proteins that activate a phosphatidylinositol-calcium second messenger
CC system.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the hypothalamus.
CC Moderate levels found in the midbrain, thalamus, medulla oblongata,
CC testis, eye, whole brain, cerebral cortex, striatum, hippocampus,
CC cerebellum, optic nerve, placenta, spinal cord, pituitary gland and
CC ovary. {ECO:0000269|PubMed:11025660}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AB040103; BAB17676.1; -; mRNA.
DR EMBL; AF268901; AAG41400.1; -; mRNA.
DR AlphaFoldDB; Q9EP86; -.
DR SMR; Q9EP86; -.
DR STRING; 10116.ENSRNOP00000000675; -.
DR BindingDB; Q9EP86; -.
DR ChEMBL; CHEMBL4571; -.
DR GlyGen; Q9EP86; 4 sites.
DR PhosphoSitePlus; Q9EP86; -.
DR PaxDb; Q9EP86; -.
DR UCSC; RGD:621570; rat.
DR RGD; 621570; Npffr1.
DR eggNOG; KOG3656; Eukaryota.
DR InParanoid; Q9EP86; -.
DR PhylomeDB; Q9EP86; -.
DR Reactome; R-RNO-389397; Orexin and neuropeptides FF and QRFP bind to their respective receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:Q9EP86; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005929; C:cilium; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0008188; F:neuropeptide receptor activity; IEA:InterPro.
DR GO; GO:0042277; F:peptide binding; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR005395; NPFF_rcpt.
DR InterPro; IPR005396; NPFF_rcpt_1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01570; NPFFRECEPTOR.
DR PRINTS; PR01571; NPFFRECEPTR1.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..432
FT /note="Neuropeptide FF receptor 1"
FT /id="PRO_0000069914"
FT TOPO_DOM 1..43
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..117
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..214
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..309
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..432
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 380..422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 116..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 432 AA; 48324 MW; 827325849444C408 CRC64;
MEAEPSQPPN GSWPLGQNGS DVETSMATSL TFSSYYQHSS PVAAMFIAAY VLIFLLCMVG
NTLVCFIVLK NRHMRTVTNM FILNLAVSDL LVGIFCMPTT LVDNLITGWP FDNATCKMSG
LVQGMSVSAS VFTLVAIAVE RFRCIVHPFR EKLTLRKALF TIAVIWALAL LIMCPSAVTL
TVTREEHHFM LDARNRSYPL YSCWEAWPEK GMRKVYTAVL FAHIYLVPLA LIVVMYVRIA
RKLCQAPGPA RDTEEAVAEG GRTSRRRARV VHMLVMVALF FTLSWLPLWV LLLLIDYGEL
SELQLHLLSV YAFPLAHWLA FFHSSANPII YGYFNENFRR GFQAAFRAQL CWPPWAAHKQ
AYSERPNRLL RRRVVVDVQP SDSGLPSESG PSSGVPGPGR LPLRNGRVAH QDGPGEGPGC
NHMPLTIPAW NI
