NPFF_HUMAN
ID NPFF_HUMAN Reviewed; 113 AA.
AC O15130; Q3SXL4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Pro-FMRFamide-related neuropeptide FF;
DE AltName: Full=FMRFamide-related peptides;
DE Contains:
DE RecName: Full=Neuropeptide SF;
DE Short=NPSF;
DE Contains:
DE RecName: Full=Neuropeptide FF;
DE Short=NPFF;
DE Contains:
DE RecName: Full=Neuropeptide AF;
DE Short=NPAF;
DE Flags: Precursor;
GN Name=NPFF;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=9224703; DOI=10.1016/s0014-5793(97)00557-7;
RA Perry S.J., Yi-Kung Huang E., Cronk D., Bagust J., Sharma R., Walker R.J.,
RA Wilson S., Burke J.F.;
RT "A human gene encoding morphine modulating peptides related to NPFF and
RT FMRFamide.";
RL FEBS Lett. 409:426-430(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION.
RX PubMed=11587714; DOI=10.1016/s0028-3908(01)00107-1;
RA Catarsi S., Babinski K., Seguela P.;
RT "Selective modulation of heteromeric ASIC proton-gated channels by
RT neuropeptide FF.";
RL Neuropharmacology 41:592-600(2001).
CC -!- FUNCTION: Morphine modulating peptides. Have wide-ranging physiologic
CC effects, including the modulation of morphine-induced analgesia,
CC elevation of arterial blood pressure, and increased somatostatin
CC secretion from the pancreas. Neuropeptide FF potentiates and sensitizes
CC ASIC1 and ASIC3 channels. {ECO:0000269|PubMed:11587714}.
CC -!- INTERACTION:
CC O15130-2; P48643: CCT5; NbExp=3; IntAct=EBI-25840002, EBI-355710;
CC O15130-2; P07339: CTSD; NbExp=3; IntAct=EBI-25840002, EBI-2115097;
CC O15130-2; P42858: HTT; NbExp=18; IntAct=EBI-25840002, EBI-466029;
CC O15130-2; P13473-2: LAMP2; NbExp=3; IntAct=EBI-25840002, EBI-21591415;
CC O15130-2; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-25840002, EBI-2623095;
CC O15130-2; O76024: WFS1; NbExp=3; IntAct=EBI-25840002, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O15130-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O15130-2; Sequence=VSP_056475;
CC -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC {ECO:0000305}.
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DR EMBL; AF005271; AAB64288.1; -; mRNA.
DR EMBL; AC023509; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471054; EAW96721.1; -; Genomic_DNA.
DR EMBL; BC104234; AAI04235.1; -; mRNA.
DR EMBL; BC104235; AAI04236.1; -; mRNA.
DR CCDS; CCDS8862.1; -. [O15130-1]
DR RefSeq; NP_001307225.1; NM_001320296.1. [O15130-2]
DR RefSeq; NP_003708.1; NM_003717.3. [O15130-1]
DR AlphaFoldDB; O15130; -.
DR BioGRID; 114175; 2.
DR IntAct; O15130; 7.
DR STRING; 9606.ENSP00000267017; -.
DR BioMuta; NPFF; -.
DR PaxDb; O15130; -.
DR PeptideAtlas; O15130; -.
DR PRIDE; O15130; -.
DR Antibodypedia; 15271; 138 antibodies from 23 providers.
DR DNASU; 8620; -.
DR Ensembl; ENST00000267017.4; ENSP00000267017.3; ENSG00000139574.9. [O15130-1]
DR GeneID; 8620; -.
DR KEGG; hsa:8620; -.
DR MANE-Select; ENST00000267017.4; ENSP00000267017.3; NM_003717.4; NP_003708.1.
DR UCSC; uc001sdw.1; human. [O15130-1]
DR CTD; 8620; -.
DR DisGeNET; 8620; -.
DR GeneCards; NPFF; -.
DR HGNC; HGNC:7901; NPFF.
DR HPA; ENSG00000139574; Tissue enriched (epididymis).
DR MIM; 604643; gene.
DR neXtProt; NX_O15130; -.
DR OpenTargets; ENSG00000139574; -.
DR PharmGKB; PA31704; -.
DR VEuPathDB; HostDB:ENSG00000139574; -.
DR eggNOG; ENOG502S60B; Eukaryota.
DR GeneTree; ENSGT00390000015021; -.
DR HOGENOM; CLU_2102411_0_0_1; -.
DR InParanoid; O15130; -.
DR OMA; GEFWSLA; -.
DR OrthoDB; 1435009at2759; -.
DR PhylomeDB; O15130; -.
DR TreeFam; TF330924; -.
DR PathwayCommons; O15130; -.
DR Reactome; R-HSA-389397; Orexin and neuropeptides FF and QRFP bind to their respective receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR SignaLink; O15130; -.
DR SIGNOR; O15130; -.
DR BioGRID-ORCS; 8620; 8 hits in 1067 CRISPR screens.
DR GeneWiki; NPFF; -.
DR GenomeRNAi; 8620; -.
DR Pharos; O15130; Tbio.
DR PRO; PR:O15130; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; O15130; protein.
DR Bgee; ENSG00000139574; Expressed in colonic epithelium and 78 other tissues.
DR Genevisible; O15130; HS.
DR GO; GO:0043679; C:axon terminus; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IBA:GO_Central.
DR GO; GO:0098794; C:postsynapse; IEA:GOC.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0005184; F:neuropeptide hormone activity; IBA:GO_Central.
DR GO; GO:0005102; F:signaling receptor binding; TAS:ProtInc.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR GO; GO:0060079; P:excitatory postsynaptic potential; IBA:GO_Central.
DR GO; GO:0007218; P:neuropeptide signaling pathway; TAS:ProtInc.
DR InterPro; IPR008065; NPFF.
DR PANTHER; PTHR15044; PTHR15044; 1.
DR Pfam; PF15085; NPFF; 1.
DR PIRSF; PIRSF038092; FMRFamid-rel_pep_precur; 1.
DR PRINTS; PR01682; FMRFAMIDEPEP.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Neuropeptide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..65
FT /id="PRO_0000009898"
FT PEPTIDE 66..76
FT /note="Neuropeptide SF"
FT /id="PRO_0000009899"
FT PEPTIDE 69..76
FT /note="Neuropeptide FF"
FT /id="PRO_0000009900"
FT PROPEP 79..92
FT /id="PRO_0000009901"
FT PEPTIDE 93..110
FT /note="Neuropeptide AF"
FT /id="PRO_0000009902"
FT REGION 22..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 76
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000250"
FT MOD_RES 110
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..34
FT /note="MDSRQAAALLVLLLLIDGGCAEGPGGQQEDQLSA -> MVPQPPTTCPWKPV
FT PSPCDLRVQGICPSSFPDTPLAQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_056475"
FT VARIANT 88
FT /note="W -> R (in dbSNP:rs35822762)"
FT /id="VAR_049183"
SQ SEQUENCE 113 AA; 12440 MW; 1E9D4ED2A69238E3 CRC64;
MDSRQAAALL VLLLLIDGGC AEGPGGQQED QLSAEEDSEP LPPQDAQTSG SLLHYLLQAM
ERPGRSQAFL FQPQRFGRNT QGSWRNEWLS PRAGEGLNSQ FWSLAAPQRF GKK
