NPG1_ARATH
ID NPG1_ARATH Reviewed; 704 AA.
AC Q8GZN1; Q9SKX3;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein NPG1 {ECO:0000303|PubMed:12928497};
DE AltName: Full=NO POLLEN GERMINATION 1 {ECO:0000303|PubMed:12928497};
GN Name=NPG1 {ECO:0000303|PubMed:12928497};
GN Synonyms=NPG {ECO:0000303|PubMed:12928497};
GN OrderedLocusNames=At2g43040 {ECO:0000312|Araport:AT2G43040};
GN ORFNames=MFL8.10 {ECO:0000312|EMBL:AAD22127.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE,
RP INTERACTION WITH CALMODULIN, DOMAIN, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12928497; DOI=10.1073/pnas.1734110100;
RA Golovkin M., Reddy A.;
RT "A calmodulin-binding protein from Arabidopsis has an essential role in
RT pollen germination.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10558-10563(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH CALMODULIN.
RX PubMed=11782485; DOI=10.1074/jbc.m111626200;
RA Reddy V.S., Ali G.S., Reddy A.S.N.;
RT "Genes encoding calmodulin-binding proteins in the Arabidopsis genome.";
RL J. Biol. Chem. 277:9840-9852(2002).
CC -!- FUNCTION: Calmodulin-binding protein essential for pollen germination,
CC but not necessary for microsporogenesis or gametogenesis
CC (PubMed:12928497). {ECO:0000269|PubMed:12928497}.
CC -!- SUBUNIT: Interacts with calmodulin in a calcium-dependent manner.
CC {ECO:0000269|PubMed:11782485, ECO:0000269|PubMed:12928497}.
CC -!- TISSUE SPECIFICITY: Expressed only in pollen and in pollen tubes.
CC {ECO:0000269|PubMed:12928497}.
CC -!- DOMAIN: The calmodulin-binding domain (CBD) is located between A-448
CC and A-496. {ECO:0000269|PubMed:12928497}.
CC -!- DISRUPTION PHENOTYPE: Normal pollen development, but no pollen
CC germination. {ECO:0000269|PubMed:12928497}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD22127.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF474176; AAO14644.1; -; mRNA.
DR EMBL; AC006224; AAD22127.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10202.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62195.1; -; Genomic_DNA.
DR EMBL; BT046199; ACI49798.1; -; mRNA.
DR PIR; C84861; C84861.
DR RefSeq; NP_001324372.1; NM_001337010.1.
DR RefSeq; NP_850382.1; NM_180051.3.
DR AlphaFoldDB; Q8GZN1; -.
DR SMR; Q8GZN1; -.
DR STRING; 3702.AT2G43040.1; -.
DR iPTMnet; Q8GZN1; -.
DR PaxDb; Q8GZN1; -.
DR PRIDE; Q8GZN1; -.
DR ProteomicsDB; 249445; -.
DR EnsemblPlants; AT2G43040.1; AT2G43040.1; AT2G43040.
DR EnsemblPlants; AT2G43040.2; AT2G43040.2; AT2G43040.
DR GeneID; 818906; -.
DR Gramene; AT2G43040.1; AT2G43040.1; AT2G43040.
DR Gramene; AT2G43040.2; AT2G43040.2; AT2G43040.
DR KEGG; ath:AT2G43040; -.
DR Araport; AT2G43040; -.
DR TAIR; locus:2053707; AT2G43040.
DR eggNOG; KOG4162; Eukaryota.
DR HOGENOM; CLU_024601_0_0_1; -.
DR InParanoid; Q8GZN1; -.
DR OMA; NRIDRWR; -.
DR OrthoDB; 167932at2759; -.
DR PhylomeDB; Q8GZN1; -.
DR PRO; PR:Q8GZN1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GZN1; baseline and differential.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IDA:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR043376; NPG1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR44102; PTHR44102; 1.
DR SMART; SM00028; TPR; 7.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 5.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..704
FT /note="Protein NPG1"
FT /id="PRO_0000438622"
FT REPEAT 24..57
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 58..90
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 177..210
FT /note="TPR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 309..342
FT /note="TPR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 430..463
FT /note="TPR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 555..588
FT /note="TPR 6"
FT /evidence="ECO:0000255"
FT REPEAT 589..622
FT /note="TPR 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 662..695
FT /note="TPR 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
SQ SEQUENCE 704 AA; 77985 MW; 45D32E8C5AC22DE3 CRC64;
MLGNQSADFS EKGEDEIVRQ LCANGICMKT TEVEAKLDEG NIQEAESSLR EGLSLNFEEA
RALLGRLEYQ RGNLEGALRV FEGIDLQAAI QRLQVSVPLE KPATKKNRPR EPQQSVSQHA
ANLVLEAIYL KAKSLQKLGR ITEAAHECKS VLDSVEKIFQ QGIPDAQVDN KLQETVSHAV
ELLPALWKES GDYQEAISAY RRALLSQWNL DNDCCARIQK DFAVFLLHSG VEASPPSLGS
QIEGSYIPRN NIEEAILLLM ILLKKFNLGK AKWDPSVFEH LTFALSLCSQ TAVLAKQLEE
VMPGVFSRIE RWNTLALSYS AAGQNSAAVN LLRKSLHKHE QPDDLVALLL AAKLCSEEPS
LAAEGTGYAQ RAINNAQGMD EHLKGVGLRM LGLCLGKQAK VPTSDFERSR LQSESLKALD
GAIAFEHNNP DLIFELGVQY AEQRNLKAAS RYAKEFIDAT GGSVLKGWRF LALVLSAQQR
FSEAEVVTDA ALDETAKWDQ GPLLRLKAKL KISQSNPTEA VETYRYLLAL VQAQRKSFGP
LRTLSQMEED KVNEFEVWHG LAYLYSSLSH WNDVEVCLKK AGELKQYSAS MLHTEGRMWE
GRKEFKPALA AFLDGLLLDG SSVPCKVAVG ALLSERGKDH QPTLPVARSL LSDALRIDPT
NRKAWYYLGM VHKSDGRIAD ATDCFQAASM LEESDPIESF STIL
