ID   NPHP4_XENLA             Reviewed;        1430 AA.
AC   B0DOB4;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Nephrocystin-4;
GN   Name=nphp4;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=26644512; DOI=10.1083/jcb.201502043;
RA   Yasunaga T., Hoff S., Schell C., Helmstaedter M., Kretz O., Kuechlin S.,
RA   Yakulov T.A., Engel C., Mueller B., Bensch R., Ronneberger O., Huber T.B.,
RA   Lienkamp S.S., Walz G.;
RT   "The polarity protein Inturned links NPHP4 to Daam1 to control the
RT   subapical actin network in multiciliated cells.";
RL   J. Cell Biol. 211:963-973(2015).
CC   -!- FUNCTION: Involved in the organization of apical junctions (By
CC       similarity). Required for building functional cilia. Involved in the
CC       organization of the subapical actin network in multiciliated epithelial
CC       cells. Seems to recruit int to basal bodies of motile cilia which
CC       subsequently interacts with actin-modifying proteins such as daam1
CC       (PubMed:26644512). May down-regulate the canonical Wnt pathway and
CC       promote the Wnt-PCP pathway. Acts as negative regulator of the hippo
CC       pathway (By similarity). {ECO:0000250|UniProtKB:O75161,
CC       ECO:0000250|UniProtKB:P59240, ECO:0000269|PubMed:26644512}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC       {ECO:0000269|PubMed:26644512}.
CC   -!- DEVELOPMENTAL STAGE: In the embryo expressed in the neural folds and
CC       the presumptive eye at stage 18. At stage 24 expressed in multiciliated
CC       cells of the epidermis. Also detected in the brain, spinal code, eye,
CC       olfactory placode, and cloaca. At stage 32 expressed in the pronephric
CC       duct, nephrostrome, cloaca, somites, posterior spinal cord, eye,
CC       branchial arch, otic vesicle, and fore-, mid-, and hindbrain.
CC       {ECO:0000269|PubMed:26644512}.
CC   -!- SIMILARITY: Belongs to the NPHP4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; B0DOB4; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0097730; C:non-motile cilium; IEA:InterPro.
DR   GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IEA:InterPro.
DR   InterPro; IPR029775; NPHP4.
DR   PANTHER; PTHR31043; PTHR31043; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT   CHAIN           1..1430
FT                   /note="Nephrocystin-4"
FT                   /id="PRO_0000437575"
FT   REGION          824..1430
FT                   /note="Sufficient for basal bodies localization"
FT                   /evidence="ECO:0000250|UniProtKB:O75161"
FT   REGION          828..857
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        828..843
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1430 AA;  161486 MW;  472A687CD6EDC19A CRC64;
     MKDWENIFSQ SHIIPPHKQR CKHAPSEKAG YQICIETLEG TPFRQGVLER SVEVSCQLRM
     TLFDSTYHHF FGRTWKSSTK PIKAIPGKAS KILFNEPIYF YTTLIDPSIM AVIEVVALSK
     KQGGSKQELS CGFGIMSLFS HQLEDDSSIQ SGRLKLYYGT PRSLLHPTLK DPNELNQHMT
     LIENTCIQYS VRPHKVLPTV AHLFPENVLV SGSETIPGIM PSCDNSGDAL KKPHVQKTVI
     CYLDQICLFL YPTLEKFEEE LTMLVNSDWL PKDDGNINGG SVSIQERRLH IGVHNGWVFV
     QKPQIVVVVP EAEMMRGHST TSLKKKNIAQ QKLSSTVQAL VLRSRIRLPE MVNHHGFALV
     FLLEYVFSIP NGIHSKTPSA ASSTSYMHMI RWASWTPSME QGSADVALPL QGGPHHNPSN
     NLVYKMPPAN MSSEEVQQVE SGTVHFTFCA GSENQLEKTR DFAETTSKEL IQSKKNVKMS
     NSKLVPETTF SPLESQVGPA LSISQLTTSP RYPAISHSSK TAMQYIPSQL LPSPMAYQLS
     HAELPYASSI THLEADMSES HPNNSNGEHL QELLFSPVHA PIVAMGTQTG STTSLLSRAS
     LARLHSVEFP EILDCNNEVA EVVDPSNPVN FNLQREEADY LLCNEIVLQF LAFTRIHDGS
     TIWPESIFFT FQFYRFHHVT TPRLQLLQLE SSDIATADPL THVLVQINKD GSIKKGSPGF
     QIKYSVDPGF LKPGERKWFL RFLALQTLQI DIWDGESLLL IGSAAVEMKH LLRQGRTAVQ
     VSHDLEVITT EYEQDLMVMS GDTAKQGTAK PIGVHTIIKG RLHMRMGNVG RPPEKKLKRR
     ETLPPSNSRI ITMHDGRTGF HGGSMLSNKS MNWKQSVCQA HKLADLDSEL AAMLCSRMKE
     VSIAYQQTNS ETDTTERRKK ERMMAVRQQE SQENANLRKS LIMAQHEERT QHTRDLQIIE
     AYRERTKPEC ISSMLNQAIT SNYTVYATLG TAEFFEFELK NPYNIQYTVT IEIDSTDLRV
     ITDTREWKHF KELTSTVTPI EENMFHVQPN TVIPQLYLRA KETVYIPFKY QTFCVDHTPM
     LQGPDAESLR KHSQLSQYKS NAMSSRRIKV SFKTSDGKLI AILQVNIEPQ PHVVDQTFRF
     YHPELTFLKK SIRLPPWYTL PGAPVGTPGG EPDLQVRCSD INIICDSKKM GPGEPQDVFI
     KVAGGPSPQI KRFFIALYID PWLAAPIQIW QIYVHSLKRV DVSCITGQLT QLSLVLRGTQ
     VVRKVRAYSS HPEELKVDPD GVFVLPPNGI QDLHIGVRPQ KAGSQFIYLN LVDVDQHQLV
     ASWLVCVSCR KPVISKAFEI ALPVGGEKGC NKRISYRNPY PTKKTYSLYT NRSDLLQFKE
     NYFEVAAGET YSIGLRFAPS QTRGQEEILI FINDREDKNE ETFCVKVRYE